Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   VDS57_RS00820 Genome accession   NZ_CP141839
Coordinates   145230..145997 (-) Length   255 a.a.
NCBI ID   WP_014477369.1    Uniprot ID   A0A0D1L7G7
Organism   Bacillus subtilis strain PN1236     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 140230..150997
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VDS57_RS00800 (VDS57_00800) ppsR 140405..141217 (-) 813 WP_003230064.1 pyruvate, water dikinase regulatory protein -
  VDS57_RS00805 (VDS57_00805) ccpN 141243..141881 (-) 639 WP_003237058.1 transcriptional regulator CcpN -
  VDS57_RS00810 (VDS57_00810) glyS 142014..144053 (-) 2040 WP_088272396.1 glycine--tRNA ligase subunit beta -
  VDS57_RS00815 (VDS57_00815) glyQ 144046..144933 (-) 888 WP_003226213.1 glycine--tRNA ligase subunit alpha -
  VDS57_RS00820 (VDS57_00820) recO 145230..145997 (-) 768 WP_014477369.1 DNA repair protein RecO Machinery gene
  VDS57_RS00825 (VDS57_00825) yqzL 146034..146177 (-) 144 WP_003230053.1 YqzL family protein -
  VDS57_RS00830 (VDS57_00830) era 146325..147230 (-) 906 WP_003230051.1 GTPase Era -
  VDS57_RS00835 (VDS57_00835) - 147211..147621 (-) 411 WP_003230049.1 cytidine deaminase -
  VDS57_RS00840 (VDS57_00840) unpK 147740..148111 (-) 372 WP_015384119.1 diacylglycerol kinase family protein -
  VDS57_RS00845 (VDS57_00845) ybeY 148092..148565 (-) 474 WP_032722131.1 rRNA maturation RNase YbeY -
  VDS57_RS00850 (VDS57_00850) pgpH 148566..150701 (-) 2136 WP_324271582.1 cyclic-di-AMP phosphodiesterase PgpH -

Sequence

Protein


Download         Length: 255 a.a.        Molecular weight: 29342.05 Da        Isoelectric Point: 8.3080

>NTDB_id=920118 VDS57_RS00820 WP_014477369.1 145230..145997(-) (recO) [Bacillus subtilis strain PN1236]
MLTKCEGIVLRTNDYGETNKIVTLLTREHGKIGVMARGAKKPNSRLSAVSQPFLYGSFLMQKTSGLGTLQQGEMILSMRG
IREDLFLTAYAAYIAELVDRGTEEKKPNPYLFEFILESLKQLNEGTDPDVITFIVQMKMLGVMGLYPELNHCVHCKSQDG
TFHFSVRDNGFICHRCFEKDPYRIPIKPQTARLLRLFYYFDLSRLGNVSLKEETKAELKQVIDLYYEEYSGLYLKSKRFL
DQMESMKHLMGENKS

Nucleotide


Download         Length: 768 bp        

>NTDB_id=920118 VDS57_RS00820 WP_014477369.1 145230..145997(-) (recO) [Bacillus subtilis strain PN1236]
ATGCTGACAAAATGTGAAGGAATCGTTCTTCGCACAAATGATTACGGAGAGACGAATAAAATCGTAACTTTACTGACAAG
AGAACACGGAAAAATAGGTGTTATGGCACGAGGCGCCAAAAAACCGAACAGCCGTTTATCAGCAGTCAGCCAGCCCTTTT
TGTATGGTTCTTTCTTAATGCAAAAAACATCGGGCCTCGGAACGCTTCAGCAGGGGGAAATGATCCTCAGCATGAGAGGC
ATCAGGGAAGACTTGTTTTTAACAGCATATGCTGCCTATATTGCTGAGTTAGTCGACAGAGGCACAGAAGAAAAAAAGCC
AAATCCTTATTTATTTGAATTCATATTAGAATCATTAAAACAGCTGAATGAAGGAACAGATCCGGATGTTATCACCTTCA
TTGTTCAAATGAAGATGCTCGGTGTCATGGGCCTGTATCCTGAGCTAAATCATTGTGTACATTGTAAAAGCCAAGACGGA
ACCTTTCATTTCTCTGTTCGTGATAATGGTTTTATATGCCACCGCTGTTTTGAAAAAGATCCTTACAGGATACCGATCAA
ACCGCAAACGGCAAGGCTGTTAAGACTCTTTTACTATTTTGATCTGTCGAGGCTCGGCAATGTGTCCTTAAAAGAGGAAA
CAAAAGCTGAATTAAAGCAAGTGATTGATTTATATTACGAAGAATATTCGGGATTGTATTTAAAATCAAAGCGCTTTTTA
GATCAAATGGAAAGCATGAAACATCTTATGGGTGAAAACAAAAGTTGA

Domains

Predicted by InterproScan.

Recombination protein O N terminal (RecO_N)

(1-75)

Recombination protein O C terminal (RecO_C)

(82-237)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0D1L7G7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

99.608

100

0.996