Detailed information NTG0919998

Bioinformatically predicted

Overview

• Name: addA
• Type: Machinery gene
• Locus tag: VO177_RS19810
• Genome accession: NZ_CP141828
• Coordinates: 3951878..3955564 (-)
• Length: 1228 a.a.
• NCBI ID: WP_031304443.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain TZS01
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 3946878..3960564

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
VO177_RS19795 (VO177_19795)	-	3946943..3947236 (-)	294	WP_021494955.1	hypothetical protein	-
VO177_RS19800 (VO177_19800)	sbcC	3947252..3950644 (-)	3393	WP_021494954.1	exonuclease subunit SbcC	-
VO177_RS19805 (VO177_19805)	sbcD	3950641..3951813 (-)	1173	WP_021494953.1	exonuclease subunit SbcD	-
VO177_RS19810 (VO177_19810)	addA	3951878..3955564 (-)	3687	WP_031304443.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
VO177_RS19815 (VO177_19815)	addB	3955551..3959051 (-)	3501	WP_021494951.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
VO177_RS19820 (VO177_19820)	-	3959192..3959905 (+)	714	WP_017417413.1	DUF421 domain-containing protein	-
VO177_RS19825 (VO177_19825)	-	3959974..3960483 (+)	510	WP_007610501.1	ferritin-like domain-containing protein	-

Sequence

Protein

• Length: 1228 a.a.
• Molecular weight: 140600.04 Da
• Isoelectric Point: 5.2183

>NTDB_id=919998 VO177_RS19810 WP_031304443.1 3951878..3955564(-) (addA) [Bacillus velezensis strain TZS01]
MQIPKPKDSIWTDDQWSAIVSSGRDILVAAAAGSGKTAVLVERMIRKITAEEDPVDVDRLLVVTFTNASAAEMKHRIAEA
LEKELVKNPGSLHIRRQLSLLNRASISTLHSFCLQVLKKYYYMIDLDPGFRMADQTEGELLGDEVLDELFEDEYAKGNQA
FFELADRYTTDRHDLDLQDLVKRVYEYSRSHPDPEAWLQSFVRLYDVTEESKMEELPFYQYVREDVEMALFGAKQKLEKA
LELTKAPGGPAPRADNFLDDLQQIEELISCRHDFDALYERVPAVSFKRAKAVKGDEFDKALLDEATDLRNGAKKLIEKVK
TDYFTRSPQDHLKSLTDMKPVIETLVQLVISYGKRFEAAKQEKSIIDFSDLEHYCLAILTAVDEEGRRVPSEAAVYYQDQ
FHEVLVDEYQDTNLVQESILQLVKSGNEEAGNLFMVGDVKQSIYRFRLAEPLLFLGKYKRFTESGEGAGQKIDLNQNFRS
RSDILDSTNFLFKQLMGGKIGEVDYDEQAALKLGASYPPNDAAKTELLLIDSAEDAEDFETVHWEAKAIAGEIRKLVSSP
FKVYDGKTKTHRNIQYRDIVILLRSMPWAPQLMEELKNQGIPVYANLTSGYFEAVEVAAALSVLKVIDNPYQDIPLASVL
RSPIVGCDENELALIRLEKKKAPFYEALKAYIANGDRHDDLYQKLRTFYDSLQKWRSFSTNHSVSELIWEVYRDTGYFDY
AGGMPGGKQRQANLRVLYDRARSYEATAFRGLFRFLRFIERMQERGDDLGTARALSEQEDVVRLMTIHSSKGLEFPVVFT
AGLGRSFNMMDLNKSYLLDKELGFGTKYIHPELRISYPTLPLVAMKKKMRRELLAEELRVLYVALTRAKEKLFLVGSCKN
REKQLAKWQAQADRADWLLSEFDRYQASSYLDFIGPALIRHRDMEAHRTPGLSSSEDIARDPSRFHIRMLQQSELLEENP
KERAEEKSKRLKAIQQGEPIPGSFSFDDQARRLLEWEYPYRELTAIRTKQSVSELKRKQEYEDEYSGRSLIKPSGDTLLY
RRPGFMMKKGLTAAEKGTAMHTVMQHIPLTHVPTAEEAERTVRMLYEKELLTEEQQEAIDIEEIVQFFGTEIGKDLLGAL
RIDREVPFSMALPAGEVYKDAETAGEPLLVQGIIDCLYETADGLYLLDYKTDRIEGKFRNGFEGAAPILQKRYETQIELY
TKAVEQITKTKVKGRALYFFDGGHVLTL

Nucleotide

• Length: 3687 bp

>NTDB_id=919998 VO177_RS19810 WP_031304443.1 3951878..3955564(-) (addA) [Bacillus velezensis strain TZS01]
ATGCAAATTCCTAAACCGAAAGACAGCATATGGACGGATGACCAATGGAGCGCCATCGTTTCTTCCGGCCGCGATATTCT
GGTCGCTGCCGCAGCCGGATCAGGTAAAACGGCCGTGCTCGTGGAACGGATGATCAGAAAGATAACCGCGGAAGAAGATC
CGGTTGATGTTGACAGGCTCCTTGTGGTGACCTTTACAAACGCCTCAGCAGCGGAGATGAAGCATCGGATCGCGGAAGCA
TTAGAAAAAGAGCTCGTCAAAAATCCGGGATCTCTGCATATCAGACGGCAGCTCTCTCTATTAAATCGGGCGAGCATTTC
GACGCTTCACTCTTTCTGTCTGCAAGTGCTTAAAAAATATTATTATATGATTGATCTTGACCCCGGTTTTCGGATGGCTG
ACCAGACGGAAGGCGAATTGCTCGGAGACGAAGTGCTTGATGAGCTGTTTGAAGATGAATACGCAAAAGGGAATCAGGCG
TTTTTTGAGCTTGCGGACAGATATACGACAGACAGGCATGATCTGGATCTTCAAGACCTTGTCAAACGGGTGTACGAGTA
TTCCCGGTCGCATCCTGACCCGGAAGCGTGGCTGCAAAGCTTTGTCCGCTTGTATGACGTGACGGAAGAAAGCAAAATGG
AAGAGCTTCCGTTTTACCAGTATGTGAGAGAAGATGTTGAAATGGCGCTCTTCGGGGCAAAACAAAAGCTTGAGAAGGCG
CTTGAACTGACAAAAGCGCCCGGCGGGCCGGCCCCGCGCGCTGATAATTTTCTGGATGACCTTCAGCAGATAGAAGAGCT
GATCAGCTGCCGGCATGACTTCGATGCGCTGTATGAACGGGTTCCCGCAGTCTCATTTAAGCGGGCGAAGGCGGTAAAGG
GTGATGAATTTGACAAAGCGCTTTTGGACGAAGCGACCGATTTGCGAAACGGCGCGAAGAAACTGATCGAAAAGGTAAAG
ACAGATTACTTTACAAGAAGTCCGCAAGATCACTTAAAGAGTTTAACAGATATGAAGCCTGTCATCGAAACGCTCGTTCA
GCTCGTGATATCGTACGGAAAACGATTTGAAGCGGCGAAACAAGAAAAATCAATTATTGATTTCTCAGATTTGGAACATT
ACTGCCTGGCGATTTTAACTGCGGTTGATGAAGAAGGCCGGCGGGTGCCGAGCGAAGCGGCCGTTTATTATCAAGACCAG
TTTCATGAAGTTCTGGTTGATGAATATCAAGATACCAATCTTGTGCAGGAGTCTATCCTGCAGCTTGTTAAAAGCGGGAA
TGAAGAGGCGGGAAATCTGTTTATGGTAGGCGACGTCAAACAATCGATCTACCGTTTCAGACTTGCTGAGCCGCTGCTGT
TTCTCGGGAAATATAAAAGGTTTACAGAAAGCGGGGAAGGCGCGGGGCAGAAAATTGATCTGAACCAAAATTTCCGCAGC
CGCTCCGATATTTTGGACAGTACGAATTTCTTGTTTAAGCAGCTGATGGGCGGAAAAATCGGCGAAGTGGACTACGATGA
ACAGGCGGCATTGAAGCTCGGCGCATCGTATCCGCCGAATGACGCGGCCAAAACGGAACTGCTGCTGATTGACAGCGCCG
AGGATGCCGAAGACTTTGAAACCGTGCACTGGGAAGCGAAAGCGATAGCCGGGGAGATCCGAAAGCTTGTCTCATCTCCG
TTTAAGGTATATGACGGCAAAACAAAAACACACCGGAATATCCAGTACCGGGATATCGTGATTTTGCTGCGTTCCATGCC
GTGGGCGCCGCAATTAATGGAAGAGCTGAAAAACCAGGGCATTCCGGTGTACGCCAATTTGACGTCGGGTTACTTTGAAG
CCGTGGAGGTGGCTGCGGCGCTTTCAGTCTTGAAGGTGATTGATAATCCGTATCAGGATATTCCGCTTGCTTCCGTGCTG
CGCTCGCCCATCGTCGGATGTGACGAAAATGAACTTGCGCTGATACGCCTTGAAAAGAAAAAAGCGCCGTTTTATGAAGC
GCTGAAAGCATATATAGCGAACGGTGACAGGCATGATGACCTGTATCAAAAACTGAGAACCTTTTATGACAGTCTGCAAA
AGTGGCGGTCATTTTCGACGAATCATTCGGTGTCTGAGCTGATTTGGGAAGTATACCGCGATACCGGATATTTTGACTAT
GCAGGCGGCATGCCTGGCGGAAAGCAGCGCCAGGCCAATCTGCGCGTCTTGTATGACCGGGCGCGCTCGTACGAAGCGAC
GGCGTTCCGCGGGTTATTCCGCTTTTTGCGGTTCATTGAACGGATGCAGGAGCGCGGCGATGATCTGGGGACGGCCCGGG
CGCTCAGCGAGCAGGAGGATGTCGTTCGGCTGATGACGATCCACAGCAGCAAAGGACTTGAATTTCCCGTCGTTTTCACC
GCCGGACTCGGCAGAAGTTTTAATATGATGGATCTCAACAAATCATACCTGCTTGATAAAGAGCTCGGTTTCGGCACGAA
GTATATTCATCCGGAACTCAGAATCAGCTATCCGACTCTGCCGCTTGTGGCGATGAAGAAAAAGATGCGCAGAGAGCTCT
TAGCGGAAGAATTGCGCGTATTGTATGTGGCGCTGACCAGAGCGAAGGAAAAGCTGTTTCTCGTCGGTTCCTGCAAAAAC
CGGGAAAAACAGCTGGCCAAATGGCAGGCGCAAGCAGACCGGGCTGATTGGCTTCTTTCTGAATTCGACCGCTATCAGGC
GTCATCTTATTTGGACTTTATCGGGCCGGCGCTCATCCGCCACCGTGACATGGAGGCGCACAGGACGCCGGGACTTTCAT
CGTCTGAGGATATCGCGCGCGACCCGTCGCGTTTTCACATCCGGATGCTGCAGCAAAGTGAATTGCTGGAAGAGAATCCA
AAAGAACGTGCTGAAGAAAAGAGCAAACGTCTCAAAGCGATTCAGCAGGGCGAGCCGATTCCTGGTTCGTTTTCATTTGA
TGATCAGGCGCGCCGTCTGCTTGAATGGGAATATCCGTACCGAGAGCTGACGGCTATCAGAACGAAGCAGTCAGTTTCTG
AGCTGAAGAGAAAGCAAGAATACGAGGATGAATACAGCGGCCGCTCTCTCATCAAACCGTCAGGAGACACGCTTTTATAC
AGGCGTCCCGGTTTTATGATGAAAAAAGGGCTGACAGCTGCCGAAAAAGGAACGGCGATGCATACGGTGATGCAGCACAT
ACCGCTGACGCACGTGCCGACTGCCGAAGAAGCGGAGCGGACGGTCCGGATGCTTTACGAAAAAGAGCTGCTGACAGAAG
AGCAGCAGGAAGCTATTGATATAGAAGAAATTGTTCAGTTTTTCGGAACTGAAATCGGTAAAGATCTTCTCGGGGCGCTG
CGGATTGACCGGGAAGTGCCGTTCAGTATGGCGCTTCCGGCCGGCGAGGTGTACAAGGATGCCGAAACGGCCGGTGAACC
GCTGCTCGTGCAGGGGATTATTGACTGCCTCTACGAAACGGCGGACGGTCTCTATTTGCTGGACTATAAAACAGATCGGA
TAGAGGGGAAATTCCGGAACGGATTTGAAGGCGCGGCTCCGATTCTGCAAAAAAGGTATGAAACACAAATCGAATTGTAT
ACAAAAGCGGTTGAACAAATTACAAAAACGAAAGTGAAAGGGCGTGCGCTCTATTTCTTCGATGGAGGTCACGTTCTTAC
ACTGTAA

Domains

Predicted by InterproScan.

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (1049-1220)

• UvrD-like helicase C-terminal domain (UvrD_C) (472-875)

• UvrD/REP helicase N-terminal domain (UvrD-helicase) (11-461)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addA	Bacillus subtilis subsp. subtilis str. 168	82.496	100	0.829