Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   VO177_RS12140 Genome accession   NZ_CP141828
Coordinates   2371717..2372787 (+) Length   356 a.a.
NCBI ID   WP_014418378.1    Uniprot ID   A0AAP4DGV9
Organism   Bacillus velezensis strain TZS01     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2366717..2377787
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VO177_RS12110 (VO177_12110) - 2367190..2367435 (-) 246 WP_003153078.1 DUF2626 domain-containing protein -
  VO177_RS12115 (VO177_12115) - 2367672..2368052 (+) 381 WP_003153079.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  VO177_RS12125 (VO177_12125) - 2368259..2369089 (+) 831 WP_021494316.1 STAS domain-containing protein -
  VO177_RS12130 (VO177_12130) - 2369127..2370428 (-) 1302 WP_021494315.1 hemolysin family protein -
  VO177_RS12135 (VO177_12135) - 2370574..2371524 (+) 951 WP_014418379.1 magnesium transporter CorA family protein -
  VO177_RS12140 (VO177_12140) comGA 2371717..2372787 (+) 1071 WP_014418378.1 competence type IV pilus ATPase ComGA Machinery gene
  VO177_RS12145 (VO177_12145) comGB 2372774..2373811 (+) 1038 WP_014418377.1 competence type IV pilus assembly protein ComGB Machinery gene
  VO177_RS12150 (VO177_12150) comGC 2373858..2374124 (+) 267 WP_050496152.1 competence type IV pilus major pilin ComGC Machinery gene
  VO177_RS12155 (VO177_12155) comGD 2374114..2374551 (+) 438 WP_007612572.1 competence type IV pilus minor pilin ComGD Machinery gene
  VO177_RS12160 (VO177_12160) comGE 2374535..2374849 (+) 315 WP_021494312.1 competence type IV pilus minor pilin ComGE Machinery gene
  VO177_RS12165 (VO177_12165) comGF 2374863..2375258 (+) 396 WP_021494311.1 competence type IV pilus minor pilin ComGF -
  VO177_RS12170 (VO177_12170) comGG 2375259..2375636 (+) 378 WP_021494310.1 competence type IV pilus minor pilin ComGG Machinery gene
  VO177_RS12175 (VO177_12175) - 2375693..2375872 (+) 180 WP_003153093.1 YqzE family protein -
  VO177_RS12180 (VO177_12180) - 2375913..2376242 (-) 330 WP_012117979.1 DUF3889 domain-containing protein -
  VO177_RS12185 (VO177_12185) tapA 2376501..2377172 (+) 672 WP_014418371.1 amyloid fiber anchoring/assembly protein TapA -
  VO177_RS12190 (VO177_12190) sipW 2377144..2377728 (+) 585 WP_012117977.1 signal peptidase I SipW -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40529.81 Da        Isoelectric Point: 9.3485

>NTDB_id=919971 VO177_RS12140 WP_014418378.1 2371717..2372787(+) (comGA) [Bacillus velezensis strain TZS01]
MKNIETYSRNIINEAYTARASDIHIVPRERDAFIHFRIGHALVKKRVLKKEECVRLISHFKFLSAMDIGERRKPQNGSLS
LPLPTETVHLRMSTLPTMNDESLVIRLLPKQQIPPLDKLSLFRGAGAALLSFLKHSHGLLLFTGPTGSGKTTTLYSLVNY
AKRHFNRRIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLSSLH
TRDAKGAIYRLLEFGVNMTEIEQTVIAIAAQRLVDLTCPFCKEENCSVYCRMYRQTRRAGIYELLYGKNLTQCFQEAKGE
HANFQYQTLRRLIRKGIALGYVTTDNYDRWVYHEAD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=919971 VO177_RS12140 WP_014418378.1 2371717..2372787(+) (comGA) [Bacillus velezensis strain TZS01]
GTGAAAAATATTGAAACATACAGCAGGAACATCATAAACGAGGCCTATACGGCAAGGGCTTCAGATATACACATTGTGCC
GAGAGAAAGGGATGCTTTCATTCATTTTCGCATCGGCCATGCTCTTGTGAAAAAAAGAGTCTTAAAAAAAGAGGAATGTG
TAAGGCTTATTTCTCATTTCAAGTTTTTATCGGCCATGGATATAGGAGAAAGACGAAAGCCGCAAAACGGGTCATTGTCT
CTGCCGCTTCCGACAGAAACCGTTCATTTGAGAATGTCAACCTTGCCGACAATGAATGATGAAAGTCTTGTCATCAGGCT
TTTGCCTAAGCAGCAGATTCCCCCTCTGGATAAACTGTCCTTATTTCGGGGGGCAGGCGCCGCATTATTGTCCTTTCTGA
AGCATTCGCACGGCCTGCTTCTGTTTACCGGCCCGACCGGATCTGGAAAAACGACCACTCTTTACTCGCTTGTCAACTAT
GCAAAACGGCATTTCAACCGGCGGATTGTCACGCTTGAAGATCCCGTAGAAACAAGAGATGAGGATGTGCTGCAAGTTCA
GGTGAATGAAAAAGCCGGAGTCACATATTCCGCCGGTTTAAAAGCGATTTTAAGACATGATCCCGATATGATCATCTTAG
GGGAAATCAGGGATGCCGAGACGGCTGAAATCGCAGTCCGTGCCGCCATGACGGGACACCTCGTTCTTTCAAGCCTGCAT
ACAAGAGATGCTAAGGGCGCCATATACAGGCTGCTGGAGTTTGGCGTGAACATGACAGAAATTGAACAAACCGTCATTGC
TATTGCCGCTCAGCGTCTGGTTGATTTAACATGTCCGTTCTGCAAAGAGGAAAACTGTTCCGTGTACTGCCGGATGTACA
GGCAGACACGCCGTGCGGGCATTTATGAACTGCTCTACGGGAAAAACCTCACCCAATGCTTTCAGGAAGCAAAGGGAGAG
CACGCCAATTTTCAATATCAGACGCTCCGCAGGCTGATCAGAAAAGGAATCGCTCTCGGCTATGTCACGACCGACAATTA
CGATCGGTGGGTGTATCATGAAGCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

82.303

100

0.823
  pilB Glaesserella parasuis strain SC1401

38.793

97.753

0.379
  pilF Thermus thermophilus HB27

37.892

98.596

0.374