Detailed information NTG0919967

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: VO177_RS11715
• Genome accession: NZ_CP141828
• Coordinates: 2295680..2296294 (+)
• Length: 204 a.a.
• NCBI ID: WP_014418419.1
• Uniprot ID: I2C7Y9
• Organism: Bacillus velezensis strain TZS01
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2290680..2301294

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
VO177_RS11680 (VO177_11680)	aroE	2291367..2292209 (+)	843	WP_003152856.1	shikimate dehydrogenase	-
VO177_RS11685 (VO177_11685)	yhbY	2292203..2292493 (+)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
VO177_RS11690 (VO177_11690)	-	2292504..2293073 (+)	570	WP_017418182.1	nicotinate-nucleotide adenylyltransferase	-
VO177_RS11695 (VO177_11695)	yqeK	2293063..2293623 (+)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
VO177_RS11700 (VO177_11700)	rsfS	2293641..2293997 (+)	357	WP_007408260.1	ribosome silencing factor	-
VO177_RS11705 (VO177_11705)	-	2293994..2294731 (+)	738	WP_021494337.1	class I SAM-dependent DNA methyltransferase	-
VO177_RS11710 (VO177_11710)	comER	2294800..2295621 (-)	822	WP_014418420.1	late competence protein ComER	-
VO177_RS11715 (VO177_11715)	comEA	2295680..2296294 (+)	615	WP_014418419.1	helix-hairpin-helix domain-containing protein	Machinery gene
VO177_RS11720 (VO177_11720)	-	2296361..2296930 (+)	570	WP_003152868.1	ComE operon protein 2	-
VO177_RS11725 (VO177_11725)	comEC	2296931..2299282 (+)	2352	WP_021494336.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
VO177_RS11730 (VO177_11730)	-	2299301..2299435 (-)	135	WP_003152870.1	YqzM family protein	-
VO177_RS11735 (VO177_11735)	-	2299476..2299627 (+)	152	Protein_2255	hypothetical protein	-
VO177_RS11740 (VO177_11740)	holA	2299667..2300708 (+)	1042	Protein_2256	DNA polymerase III subunit delta	-
VO177_RS11745 (VO177_11745)	rpsT	2300725..2300991 (-)	267	WP_021494335.1	30S ribosomal protein S20	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22226.26 Da
• Isoelectric Point: 5.0355

>NTDB_id=919967 VO177_RS11715 WP_014418419.1 2295680..2296294(+) (comEA) [Bacillus velezensis strain TZS01]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMEAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTTAGEKENAAVNINTASLDELQAVSGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=919967 VO177_RS11715 WP_014418419.1 2295680..2296294(+) (comEA) [Bacillus velezensis strain TZS01]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCTGCAGGAGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGGAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAA
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGGTTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

3D structure

• AlphaFold DB: I2C7Y9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.333	94.118	0.549
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363