Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   VO177_RS02165 Genome accession   NZ_CP141828
Coordinates   444153..444590 (+) Length   145 a.a.
NCBI ID   WP_103527166.1    Uniprot ID   -
Organism   Bacillus velezensis strain TZS01     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 439153..449590
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VO177_RS02150 (VO177_02150) - 442039..442401 (-) 363 WP_007409352.1 winged helix-turn-helix transcriptional regulator -
  VO177_RS02155 (VO177_02155) hxlA 442633..443268 (+) 636 WP_014416875.1 3-hexulose-6-phosphate synthase -
  VO177_RS02160 (VO177_02160) hxlB 443265..443822 (+) 558 WP_103527165.1 6-phospho-3-hexuloisomerase -
  VO177_RS02165 (VO177_02165) nucA/comI 444153..444590 (+) 438 WP_103527166.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  VO177_RS02170 (VO177_02170) nin/comJ 444611..445009 (+) 399 WP_007609299.1 competence protein ComJ Regulator
  VO177_RS02175 (VO177_02175) - 445050..446486 (-) 1437 WP_014416872.1 glycoside hydrolase family 1 protein -
  VO177_RS02180 (VO177_02180) - 446590..446868 (-) 279 WP_263612330.1 YckD family protein -
  VO177_RS02185 (VO177_02185) - 446985..447419 (-) 435 WP_014416871.1 RDD family protein -
  VO177_RS02190 (VO177_02190) - 447434..448570 (-) 1137 WP_094296367.1 zinc-dependent alcohol dehydrogenase -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16045.28 Da        Isoelectric Point: 7.9203

>NTDB_id=919934 VO177_RS02165 WP_103527166.1 444153..444590(+) (nucA/comI) [Bacillus velezensis strain TZS01]
MKTLKALLLVLIIIAGAVAALIKGDLFSDNEQTVQKSDYDKVILFPSDRYPETAKHIKDAITEGHSSICTIDRDGVHKRR
EQSLKHVPVKTGYDRDEWPMAVCKQGGNNASVEYISPADNRGAGSWVGHQLTNDPDGTRVLFKIK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=919934 VO177_RS02165 WP_103527166.1 444153..444590(+) (nucA/comI) [Bacillus velezensis strain TZS01]
TTGAAAACATTAAAAGCACTGCTGCTTGTTCTTATCATCATTGCCGGAGCAGTCGCGGCCCTGATCAAGGGTGATCTTTT
CTCAGATAACGAGCAGACCGTTCAGAAGAGTGATTACGACAAGGTAATCCTATTTCCGTCCGATCGATATCCGGAAACAG
CAAAGCATATTAAGGACGCGATAACAGAAGGACATTCCAGCATATGCACCATTGACAGAGACGGAGTACATAAGCGGCGT
GAGCAATCTCTTAAACATGTGCCTGTCAAAACGGGATACGATAGGGATGAATGGCCGATGGCGGTTTGTAAACAGGGCGG
AAATAATGCCTCCGTAGAATATATTTCTCCGGCAGACAACCGCGGAGCCGGCTCTTGGGTCGGACACCAGCTTACCAATG
ATCCCGACGGAACGAGAGTATTATTTAAAATCAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

73.81

86.897

0.641