Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   VCT43_RS03555 Genome accession   NZ_CP141808
Coordinates   746332..747312 (+) Length   326 a.a.
NCBI ID   WP_047738304.1    Uniprot ID   -
Organism   Enterobacter hormaechei strain WD01     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 741332..752312
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VCT43_RS03515 (VCT43_03515) - 741417..741743 (+) 327 WP_003862421.1 YggL family protein -
  VCT43_RS03520 (VCT43_03520) - 741800..742516 (+) 717 WP_003862419.1 DUF2884 domain-containing protein -
  VCT43_RS03525 (VCT43_03525) - 742600..743028 (-) 429 WP_409013749.1 endonuclease domain-containing protein -
  VCT43_RS03530 (VCT43_03530) hemW 742994..744142 (-) 1149 WP_017382926.1 radical SAM family heme chaperone HemW -
  VCT43_RS03535 (VCT43_03535) - 744135..744728 (-) 594 WP_039269793.1 XTP/dITP diphosphatase -
  VCT43_RS03540 (VCT43_03540) yggU 744732..745028 (-) 297 WP_023304404.1 DUF167 family protein YggU -
  VCT43_RS03545 (VCT43_03545) - 745025..745591 (-) 567 WP_015571838.1 YggT family protein -
  VCT43_RS03550 (VCT43_03550) - 745613..746314 (-) 702 WP_047726777.1 YggS family pyridoxal phosphate-dependent enzyme -
  VCT43_RS03555 (VCT43_03555) pilT 746332..747312 (+) 981 WP_047738304.1 type IV pilus twitching motility protein PilT Machinery gene
  VCT43_RS03560 (VCT43_03560) ruvX 747322..747738 (-) 417 WP_006811925.1 Holliday junction resolvase RuvX -
  VCT43_RS03565 (VCT43_03565) - 747738..748298 (-) 561 WP_006811924.1 YqgE/AlgH family protein -
  VCT43_RS03570 (VCT43_03570) gshB 748373..749320 (-) 948 WP_047738306.1 glutathione synthase -
  VCT43_RS03575 (VCT43_03575) rsmE 749352..750083 (-) 732 WP_045333606.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  VCT43_RS03580 (VCT43_03580) endA 750135..750842 (-) 708 WP_003860034.1 deoxyribonuclease I -
  VCT43_RS03585 (VCT43_03585) - 750937..751434 (-) 498 WP_015571833.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35663.66 Da        Isoelectric Point: 6.2335

>NTDB_id=919617 VCT43_RS03555 WP_047738304.1 746332..747312(+) (pilT) [Enterobacter hormaechei strain WD01]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRSGRLEPAPFPPPDVEALLKAWLNDEQQGAWWANGQVDFAVTLAGRQRLRG
SAFKHMHGVSIALRLLPLTCPQLSALGVPRAIPELLSNDNGLILVTGATGSGKSTTLAAMVDFLNHQTDGHILTLEDPVE
FMYQSERCLIQQREIGQHSPSFAEALRSALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQKLLQDVQGGRVALYELLVNTSAAANLIREGKTWQLPGIIQTGQQAGMQNFEQSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=919617 VCT43_RS03555 WP_047738304.1 746332..747312(+) (pilT) [Enterobacter hormaechei strain WD01]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCGCCACCGCG
CTGGCGCAGGTCAGGCCGTCTTGAACCTGCGCCGTTTCCGCCCCCGGATGTGGAGGCGTTATTAAAAGCGTGGCTCAACG
ACGAACAGCAGGGCGCATGGTGGGCAAACGGGCAGGTGGATTTTGCCGTTACCCTCGCAGGCCGTCAGCGGCTGCGCGGC
AGTGCGTTTAAGCATATGCACGGCGTTTCGATTGCGCTGCGGCTGTTGCCGCTGACGTGCCCGCAGCTCTCTGCGTTAGG
TGTGCCGCGAGCGATCCCGGAGCTGCTGTCCAATGACAATGGCCTGATTCTGGTCACTGGCGCCACCGGCAGCGGGAAAT
CGACCACCCTGGCCGCGATGGTCGATTTCCTCAATCACCAGACGGACGGGCATATTCTGACCCTGGAAGATCCGGTGGAG
TTTATGTACCAGAGCGAACGTTGCCTGATCCAGCAGCGGGAGATAGGCCAGCACAGCCCGTCCTTTGCCGAGGCGCTGCG
CAGCGCGCTGCGTGAAGATCCGGATGTGATTCTGCTTGGTGAGCTGCGCGACAGCGAGACGATACGCCTGGCGCTGACGG
CGGCGGAAACCGGCCATCTGGTGTTAGCCACGCTGCATACGCGCGGCGCGGCGCAGGCGATCGAGCGCCTGGTCGACACC
TTTCCGGCGCAGGAGAAAGATCCGGTGCGTAATCAGCTGGCGGGAAGCCTGCGTGCGGTCCTGGCGCAGAAGCTACTTCA
GGATGTCCAGGGCGGGCGCGTGGCGCTGTATGAGCTACTGGTGAATACCTCGGCGGCGGCAAACCTGATCCGCGAAGGCA
AAACCTGGCAACTGCCGGGCATTATTCAAACGGGGCAGCAGGCGGGAATGCAGAACTTTGAGCAGAGTCTGGCGGAGCGA
CGGGCGCAGGGGCGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(89-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.765

100

0.509
  pilT Vibrio cholerae strain A1552

50.765

100

0.509
  pilT Pseudomonas stutzeri DSM 10701

48.318

100

0.485
  pilT Legionella pneumophila strain Lp02

49.068

98.773

0.485
  pilT Legionella pneumophila strain ERS1305867

49.068

98.773

0.485
  pilT Neisseria meningitidis 8013

47.866

100

0.482
  pilT Neisseria gonorrhoeae MS11

47.561

100

0.479
  pilT Acinetobacter baumannii strain A118

47.401

100

0.475
  pilT Acinetobacter baumannii D1279779

47.401

100

0.475
  pilT Acinetobacter baylyi ADP1

47.095

100

0.472
  pilT Pseudomonas aeruginosa PAK

47.095

100

0.472
  pilT Acinetobacter nosocomialis M2

47.812

98.16

0.469
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.168

98.773

0.426
  pilU Pseudomonas stutzeri DSM 10701

38.182

100

0.387
  pilU Vibrio cholerae strain A1552

38.199

98.773

0.377