Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   VDS54_RS01515 Genome accession   NZ_CP141807
Coordinates   316552..317925 (+) Length   457 a.a.
NCBI ID   WP_320531658.1    Uniprot ID   -
Organism   Limosilactobacillus fermentum strain JL-2     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 311552..322925
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VDS54_RS01485 (VDS54_01485) - 311553..312428 (+) 876 WP_320531655.1 alpha/beta hydrolase -
  VDS54_RS01490 (VDS54_01490) - 312745..314082 (-) 1338 WP_320531656.1 C1 family peptidase -
  VDS54_RS01495 (VDS54_01495) - 314146..314760 (-) 615 Protein_276 sugar O-acetyltransferase -
  VDS54_RS01500 (VDS54_01500) rpiA 314791..315477 (-) 687 WP_021815661.1 ribose-5-phosphate isomerase RpiA -
  VDS54_RS01505 (VDS54_01505) - 315489..315800 (-) 312 WP_021815662.1 GNAT family N-acetyltransferase -
  VDS54_RS01510 (VDS54_01510) - 316000..316539 (+) 540 WP_320531657.1 dUTP diphosphatase -
  VDS54_RS01515 (VDS54_01515) radA 316552..317925 (+) 1374 WP_320531658.1 DNA repair protein RadA Machinery gene
  VDS54_RS01520 (VDS54_01520) - 317958..319091 (+) 1134 WP_046948740.1 PIN/TRAM domain-containing protein -
  VDS54_RS01525 (VDS54_01525) gltX 319162..320658 (+) 1497 WP_021815666.1 glutamate--tRNA ligase -
  VDS54_RS01530 (VDS54_01530) - 320726..321295 (+) 570 WP_046948741.1 TIGR01440 family protein -

Sequence

Protein


Download         Length: 457 a.a.        Molecular weight: 49343.70 Da        Isoelectric Point: 8.7358

>NTDB_id=919600 VDS54_RS01515 WP_320531658.1 316552..317925(+) (radA) [Limosilactobacillus fermentum strain JL-2]
MAKAKTHFVCQNCGYNSPRYLGRCPNCGQWSTLVEEVEQASTPAAKNATATLTGIVARPQRIQEIDTKKTPRVKTRLNEL
NRVLGGGIVPGSLVLIGGDPGIGKSTLLLQVSGQLSVEHHKVLYVSGEESASQIKLRAQRLDVSGEDFYIYPETNMEAIR
ATIDSIQPEFVIIDSVQTMQAADVTSAIGSVSQIRAVTAQLMQIAKGQNITVFVVGHVTKGGALAGPKILEHMVDTVLYF
EGDLHHTYRILRSVKNRFGSTNELGIFEMNTRGLTEVANPSEIFLEERLKDATGSAVVVSLEGTRPILVEIQALITPTVF
GNAQRTATGLNRNRVSLIMAVLEKRANLLLQNQDAYLKAAGGVKLDEPAIDLAIAVAIASSYRDKGTRPTDAFVGEVGLT
GEIRRVSRIEQRVAEAAKLGFKRILVPKNNLAGWNPSQDIEVVGVTTLGEALKIALG

Nucleotide


Download         Length: 1374 bp        

>NTDB_id=919600 VDS54_RS01515 WP_320531658.1 316552..317925(+) (radA) [Limosilactobacillus fermentum strain JL-2]
ATGGCAAAAGCCAAAACACACTTTGTCTGCCAAAACTGCGGTTACAATTCACCGCGCTATTTAGGGCGGTGTCCCAATTG
TGGCCAGTGGAGTACCTTAGTTGAAGAAGTCGAGCAGGCAAGTACCCCGGCGGCTAAAAACGCTACGGCAACCTTAACCG
GGATTGTGGCCCGGCCCCAGCGAATCCAAGAAATTGACACCAAAAAAACGCCCCGGGTTAAGACCCGCTTAAATGAGTTA
AACCGGGTGCTAGGTGGCGGAATTGTACCCGGCTCGTTGGTCTTAATTGGTGGGGATCCCGGGATCGGGAAGTCCACCCT
GCTCTTACAAGTGTCGGGGCAACTGAGCGTGGAACACCACAAGGTTCTTTACGTTTCCGGGGAAGAATCGGCCTCCCAAA
TTAAATTGCGGGCCCAACGCTTAGACGTTTCCGGCGAGGACTTTTATATCTACCCGGAAACCAACATGGAAGCCATCCGG
GCGACGATTGATTCCATTCAGCCCGAGTTTGTAATCATTGACTCGGTTCAGACGATGCAGGCGGCCGACGTGACCTCGGC
GATTGGGTCGGTCTCCCAAATCCGGGCGGTCACCGCCCAGTTGATGCAGATTGCCAAGGGGCAAAACATCACGGTCTTCG
TGGTTGGTCACGTCACCAAGGGTGGGGCCTTAGCCGGTCCCAAGATCTTGGAACACATGGTTGACACGGTGCTGTACTTT
GAAGGGGACCTCCACCACACCTACCGGATTTTACGGTCGGTCAAAAACCGGTTTGGGTCCACCAATGAGTTAGGGATCTT
CGAAATGAATACCCGTGGTTTGACCGAGGTCGCAAATCCCTCGGAAATCTTTTTGGAAGAGCGGCTTAAGGATGCCACCG
GGTCGGCCGTGGTCGTATCCCTGGAGGGAACCCGACCGATCCTGGTTGAAATCCAGGCCTTGATCACGCCAACCGTCTTT
GGAAACGCCCAAAGAACGGCGACGGGTTTAAACCGCAACCGGGTTTCCTTGATCATGGCGGTCCTAGAAAAACGGGCGAA
CCTGCTCTTACAAAACCAGGATGCCTACTTGAAGGCGGCCGGGGGCGTTAAGTTAGACGAGCCGGCCATCGACCTTGCGA
TCGCGGTGGCAATTGCTTCTTCTTACCGGGATAAGGGGACCCGGCCGACCGACGCCTTCGTCGGTGAAGTTGGCTTAACC
GGGGAGATCCGTCGGGTGAGCCGAATTGAACAGCGGGTGGCCGAAGCCGCTAAACTAGGCTTTAAGCGGATTTTAGTGCC
CAAAAACAATTTAGCGGGTTGGAACCCGTCGCAAGACATCGAAGTCGTGGGGGTAACGACCCTTGGCGAAGCACTTAAAA
TTGCCCTTGGATAG

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(64-222)

Subunit ChlI of Mg-chelatase (ChlI)

(364-430)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis SK321

67.991

99.125

0.674
  radA Streptococcus mitis NCTC 12261

67.77

99.125

0.672
  radA Streptococcus pneumoniae Rx1

67.77

99.125

0.672
  radA Streptococcus pneumoniae D39

67.77

99.125

0.672
  radA Streptococcus pneumoniae R6

67.77

99.125

0.672
  radA Streptococcus pneumoniae TIGR4

67.77

99.125

0.672
  radA Bacillus subtilis subsp. subtilis str. 168

63.457

100

0.635