Detailed information    

insolico Bioinformatically predicted

Overview

Name   priA   Type   Machinery gene
Locus tag   ABU640_RS01145 Genome accession   NZ_CP161854
Coordinates   248947..251151 (+) Length   734 a.a.
NCBI ID   WP_001908529.1    Uniprot ID   -
Organism   Vibrio cholerae strain C3616     
Function   DNA puliing through the inner membrane (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 243947..256151
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABU640_RS01125 (ABU640_01125) - 245027..246193 (-) 1167 WP_000213242.1 O-succinylhomoserine (thiol)-lyase -
  ABU640_RS01130 (ABU640_01130) metJ 246461..246778 (+) 318 WP_000796267.1 met regulon transcriptional regulator MetJ -
  ABU640_RS01135 (ABU640_01135) - 246889..248166 (-) 1278 WP_001278588.1 malic enzyme-like NAD(P)-binding protein -
  ABU640_RS01140 (ABU640_01140) rpmE 248474..248692 (-) 219 WP_000643446.1 50S ribosomal protein L31 -
  ABU640_RS01145 (ABU640_01145) priA 248947..251151 (+) 2205 WP_001908529.1 primosomal protein N' Machinery gene
  ABU640_RS01150 (ABU640_01150) cytR 251457..252464 (+) 1008 WP_000224452.1 DNA-binding transcriptional regulator CytR Regulator
  ABU640_RS01155 (ABU640_01155) - 252636..253187 (+) 552 WP_032475230.1 SPOR domain-containing protein -
  ABU640_RS01160 (ABU640_01160) hslV 253324..253881 (+) 558 WP_000208247.1 ATP-dependent protease subunit HslV -
  ABU640_RS01165 (ABU640_01165) hslU 253947..255278 (+) 1332 WP_096070311.1 HslU--HslV peptidase ATPase subunit -

Sequence

Protein


Download         Length: 734 a.a.        Molecular weight: 82052.37 Da        Isoelectric Point: 8.4848

>NTDB_id=919208 ABU640_RS01145 WP_001908529.1 248947..251151(+) (priA) [Vibrio cholerae strain C3616]
MRPTIARVCLPVPLDKSFDYLIPAHLFPVLGGRVQVPFGRQTLVGIVQSLTHQSDFPIEQLKSVQAVLDDAPVWPDKLQS
LLHWCSQFYHYPLGETYANALPGALRKGKAAELTSHKEWRLTALGQEQLMQGVKRGAVKQAQVLHLLQHGALSHQTLLDE
EVSSSTLKALVEKGWIECEERKPVARPWPQELEAKVDKPKLNQEQAIAIAAVNSQQGFGCFLLEGVTGSGKTEVYLNLIT
PVLARGEQALVLVPEIGLTPQTINRFRQRFNVPVEVMHSALNDTERLNAWLAARDKVAGIVIGTRSALLTPFAKLGIIIV
DEEHDSSYKQQDSLRYHARDVAVMRAHLENIPIVLGSATPALETLHNALSGKYHHLQLTQRAGNALPTRNHALDVKGLYL
ESGLSAPLIAEMRRHLSAGNQVMLFLNRRGFSPALMCHECGWIAECQRCDAYYTYHQHSNEMRCHHCGSQRPVLHQCKGC
GSTQLVTVGVGTEQLEAQLHTLFPEYRSVRIDRDSTRRKGSLESALTAIRKGEYQILIGTQMLAKGHHFPDVTLVALLDV
DGSLYSSDFRASERLAQLFIQVAGRAGRASKPGEVVLQTHHPEHSLLQALLHKDYHHFALTALEERKLAQLPPYSFLSLF
RAEANHTAQVEDFLRQVRETLRCNPWFDSECMVLGPTPAPLAKRAGKFRWQLLLQTPNRTLMQKILQSARPALQQLPLAS
KVRWSIDIDPQDLS

Nucleotide


Download         Length: 2205 bp        

>NTDB_id=919208 ABU640_RS01145 WP_001908529.1 248947..251151(+) (priA) [Vibrio cholerae strain C3616]
ATGCGACCCACCATTGCCCGAGTTTGCCTGCCTGTACCTCTGGATAAGTCTTTCGACTATCTGATCCCTGCTCATCTATT
TCCAGTGCTGGGCGGTCGTGTACAAGTGCCTTTTGGGCGACAAACCTTGGTCGGCATTGTCCAGTCGCTGACACATCAAT
CTGATTTTCCTATCGAACAACTGAAATCCGTGCAAGCCGTTTTGGATGACGCGCCCGTGTGGCCAGATAAACTGCAGTCT
CTGCTGCACTGGTGTAGTCAGTTTTATCATTATCCGCTCGGAGAGACGTACGCGAATGCCTTGCCCGGCGCACTGCGCAA
AGGCAAGGCGGCAGAGTTGACTAGCCATAAGGAGTGGCGTTTGACCGCGCTTGGTCAAGAGCAATTGATGCAAGGGGTGA
AGCGTGGCGCAGTAAAGCAAGCGCAAGTCCTTCACCTGCTGCAGCACGGCGCTTTATCGCACCAAACTTTGCTTGATGAA
GAGGTGAGCAGTTCGACCCTCAAAGCCTTGGTCGAAAAAGGGTGGATCGAGTGTGAAGAGCGTAAACCTGTGGCGCGTCC
TTGGCCGCAAGAGCTGGAAGCTAAAGTCGATAAACCTAAGCTTAATCAAGAGCAGGCGATCGCGATTGCGGCGGTGAATA
GCCAGCAAGGTTTCGGCTGCTTTTTATTAGAAGGGGTAACGGGCTCAGGCAAAACAGAGGTGTATCTTAACCTCATCACC
CCTGTATTGGCGCGTGGTGAACAAGCCTTGGTGTTGGTGCCGGAAATTGGTTTAACTCCACAAACCATCAATCGTTTTCG
CCAACGTTTTAATGTCCCCGTCGAAGTGATGCACTCGGCGCTCAACGACACTGAACGCCTCAACGCTTGGCTGGCGGCGC
GCGATAAAGTGGCGGGGATTGTGATTGGTACGCGCTCGGCGCTGCTCACACCCTTTGCCAAATTGGGCATTATTATTGTT
GATGAAGAACATGATAGTTCTTACAAGCAGCAAGATAGCCTGCGTTATCACGCCCGCGATGTGGCTGTGATGCGCGCGCA
TCTGGAGAATATTCCGATTGTGCTTGGCTCGGCGACGCCAGCCTTGGAAACGTTGCATAATGCGCTCAGTGGTAAATACC
ATCACTTGCAACTGACGCAGCGCGCCGGTAATGCACTACCCACACGTAACCATGCGTTGGATGTGAAAGGGCTCTATCTA
GAAAGTGGCCTGTCTGCGCCTTTGATTGCGGAAATGCGCCGCCATTTGTCGGCGGGCAATCAGGTGATGCTGTTTTTAAA
CCGCCGCGGTTTTTCTCCGGCCTTGATGTGTCACGAGTGTGGCTGGATTGCGGAGTGCCAGCGCTGTGATGCCTATTACA
CCTACCATCAACACAGCAATGAAATGCGCTGCCACCATTGTGGTTCACAGCGTCCTGTTCTCCATCAATGCAAAGGGTGC
GGCTCGACTCAACTGGTTACTGTCGGCGTTGGTACGGAACAGCTTGAAGCGCAATTACACACTCTATTCCCTGAGTATCG
TTCAGTGCGGATTGATCGAGATAGCACGCGGCGTAAAGGCAGTTTGGAATCGGCGTTAACCGCGATTCGCAAAGGCGAAT
ATCAGATTTTGATTGGTACGCAAATGCTCGCCAAAGGACATCACTTCCCCGATGTTACCTTGGTCGCGTTGTTGGATGTC
GATGGCTCATTGTACAGCAGTGATTTTCGCGCTTCTGAGCGTTTGGCTCAGCTCTTTATTCAAGTCGCAGGGCGGGCAGG
GCGTGCCAGCAAGCCGGGCGAAGTGGTGCTACAAACGCATCATCCCGAACACAGTTTGCTACAAGCCTTGCTGCATAAGG
ATTACCACCATTTTGCCCTGACGGCGTTGGAAGAGCGCAAGCTGGCGCAGTTGCCCCCTTATTCTTTCTTGAGCTTATTT
CGAGCCGAAGCCAATCACACCGCTCAAGTGGAAGATTTTTTGCGTCAAGTGCGCGAAACCTTGCGCTGCAATCCTTGGTT
TGATAGCGAATGCATGGTGCTCGGGCCAACGCCTGCTCCACTCGCGAAACGAGCGGGAAAATTCCGTTGGCAACTTTTAC
TACAAACGCCGAATCGCACCCTGATGCAGAAGATTTTGCAAAGCGCACGTCCAGCACTGCAACAATTACCGCTGGCGAGC
AAGGTGCGCTGGTCGATCGATATTGATCCGCAAGATTTGAGCTAA

Domains

Predicted by InterProScan.

DEAD/DEAH box helicase (DEAD)

(205-364)

Primosomal protein N C-terminal domain (PriA_C)

(633-730)

3DNA-binding domain (3BD) (PriA_3primeBD)

(7-102)

Helicase conserved C-terminal domain (Helicase_C)

(515-589)

PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA)

(446-472)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  priA Acinetobacter baumannii D1279779

43.572

100

0.439