Detailed information    

insolico Bioinformatically predicted

Overview

Name   comM   Type   Machinery gene
Locus tag   VNI85_RS28155 Genome accession   NZ_CP141735
Coordinates   5424947..5426467 (+) Length   506 a.a.
NCBI ID   WP_012969207.1    Uniprot ID   A0A1F2LT85
Organism   Klebsiella variicola strain 2023CK-01508     
Function   require for natural transformation (predicted from homology)   
Unclear

Genomic Context

Location: 5419947..5431467
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VNI85_RS28135 (VNI85_28145) - 5421504..5422433 (-) 930 WP_008807980.1 branched-chain amino acid transaminase -
  VNI85_RS28140 (VNI85_28150) ilvM 5422453..5422710 (-) 258 WP_008807981.1 acetolactate synthase 2 small subunit -
  VNI85_RS28145 (VNI85_28155) ilvG 5422707..5424353 (-) 1647 WP_338260679.1 acetolactate synthase 2 catalytic subunit -
  VNI85_RS28150 (VNI85_28160) ilvL 5424494..5424592 (-) 99 WP_001311244.1 ilv operon leader peptide -
  VNI85_RS28155 (VNI85_28165) comM 5424947..5426467 (+) 1521 WP_012969207.1 YifB family Mg chelatase-like AAA ATPase Machinery gene
  VNI85_RS28160 (VNI85_28170) - 5426492..5426830 (-) 339 WP_004886877.1 DUF413 domain-containing protein -
  VNI85_RS28165 (VNI85_28175) hdfR 5426949..5427770 (+) 822 WP_012543255.1 HTH-type transcriptional regulator HdfR -

Sequence

Protein


Download         Length: 506 a.a.        Molecular weight: 55099.54 Da        Isoelectric Point: 8.2651

>NTDB_id=919086 VNI85_RS28155 WP_012969207.1 5424947..5426467(+) (comM) [Klebsiella variicola strain 2023CK-01508]
MSLAIVYTRAALGIEAPLITIEVHLSSGLPGLTMVGLPETTVKEARDRVRSALINSGYAFPAKKITINLAPADLPKEGGR
YDLPIALALLVASEQLNTTRLNQYEFVGELALTGGLRGVPGAIPSAMEAIKAGRRIVVSSDNAAEVGLIGGSDCLVADHL
QQVCAFLAGQASLSPPLADVPAVDERCEDLRDVIGQQQGKRALEIVAAGGHNLLLIGPPGTGKTMLACRLPGLLPPLSNQ
EALESTAIQSLVNLQTAKTRWRQRPFRAPHHSASLAAMVGGGSIPVPGEISLAHNGVLFLDELPEFERRVLDALREPIES
GKIHISRSRAKIDYPARFQLIAAMNPSPTGHYQGKHNRASPEQTLRYLGRLSGPFLDRFDLSLEIPLPPPGILSQGTQGE
ESSATVRQRVLAARERQMLRQNKLNAHLENREMKSCCHLRQEDALWLEQTLTQLGLSIRAWQRLLKVARTIADLAEAEEI
ERRHLQEALSYRAIDRMLNHLQKMMA

Nucleotide


Download         Length: 1521 bp        

>NTDB_id=919086 VNI85_RS28155 WP_012969207.1 5424947..5426467(+) (comM) [Klebsiella variicola strain 2023CK-01508]
ATGTCGCTCGCCATCGTTTACACACGCGCGGCGCTCGGTATCGAAGCGCCATTGATTACTATCGAGGTTCATCTCAGCAG
CGGTCTGCCTGGCTTGACCATGGTCGGGCTACCGGAGACCACCGTGAAAGAGGCCCGCGACCGGGTACGCAGCGCCTTGA
TCAACAGCGGCTACGCTTTCCCTGCGAAGAAAATAACCATTAACCTGGCACCCGCGGATCTGCCCAAAGAGGGCGGGCGA
TATGACCTTCCCATCGCTCTCGCGCTTCTCGTCGCCTCAGAGCAGCTCAACACTACCCGATTGAATCAATATGAGTTTGT
GGGCGAACTCGCCCTTACAGGCGGCTTACGCGGCGTTCCAGGGGCGATCCCCAGCGCAATGGAAGCCATCAAAGCCGGCC
GGCGCATTGTCGTCTCCTCTGACAATGCGGCTGAGGTGGGTCTGATCGGCGGCAGCGATTGCCTGGTCGCCGACCATCTG
CAACAGGTGTGCGCGTTTCTCGCAGGGCAAGCATCGCTCTCGCCGCCTCTCGCCGACGTGCCCGCCGTTGATGAACGCTG
TGAAGATCTACGTGATGTTATCGGCCAACAACAAGGCAAGCGAGCGCTGGAGATTGTGGCGGCCGGTGGCCACAACCTGC
TGCTGATTGGCCCGCCCGGTACCGGCAAAACAATGCTTGCCTGCCGGCTCCCCGGCCTTTTGCCGCCATTAAGCAACCAG
GAAGCGCTGGAGAGCACGGCCATTCAAAGTCTGGTCAACCTCCAGACTGCGAAGACCCGGTGGCGTCAGAGGCCGTTTCG
CGCCCCTCACCATAGCGCCTCGCTGGCAGCGATGGTAGGCGGAGGCTCAATACCTGTCCCCGGCGAGATTTCACTGGCCC
ATAATGGCGTCCTATTTCTTGATGAACTGCCAGAGTTTGAACGACGGGTGCTGGACGCGCTGCGTGAACCCATTGAGTCA
GGCAAGATCCACATATCACGATCGCGCGCCAAGATTGACTATCCGGCGCGTTTTCAGCTTATCGCGGCAATGAACCCAAG
CCCTACCGGGCATTATCAGGGCAAACATAATCGCGCGTCGCCTGAGCAGACATTGCGCTACCTTGGGCGCCTGTCCGGCC
CTTTTCTCGACCGCTTCGATCTTTCTTTAGAGATCCCGCTCCCGCCGCCGGGGATACTGAGCCAGGGCACGCAGGGCGAA
GAATCGAGTGCGACGGTCCGTCAGCGTGTGCTGGCGGCACGTGAACGTCAAATGCTCAGGCAAAATAAGCTGAATGCGCA
TCTTGAGAATCGTGAAATGAAGAGCTGCTGTCACTTGCGCCAGGAGGATGCGCTTTGGCTGGAACAGACGTTAACGCAGC
TGGGGCTTTCTATACGGGCCTGGCAGCGTCTGTTAAAGGTGGCAAGAACCATTGCCGATCTGGCAGAAGCTGAAGAGATT
GAACGCCGCCATTTGCAGGAGGCGCTCAGCTATCGGGCAATAGACCGGATGCTAAACCATCTGCAGAAAATGATGGCGTA
A

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(21-142)

Magnesium chelatase, subunit ChlI C-terminal (Mg_chelatase_C)

(400-492)

Magnesium chelatase, subunit ChlI (Mg_chelatase)

(189-386)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1F2LT85

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comM Haemophilus influenzae Rd KW20

59.883

100

0.605
  comM Glaesserella parasuis strain SC1401

59.766

100

0.605
  comM Vibrio cholerae strain A1552

59.245

99.407

0.589
  comM Vibrio campbellii strain DS40M4

58.532

99.605

0.583
  comM Legionella pneumophila str. Paris

49.497

98.221

0.486
  comM Legionella pneumophila strain ERS1305867

49.497

98.221

0.486
  RA0C_RS07335 Riemerella anatipestifer ATCC 11845 = DSM 15868

44.862

100

0.449