Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ABXJ20_RS17325 Genome accession   NZ_CP161803
Coordinates   3554225..3555658 (+) Length   477 a.a.
NCBI ID   WP_000809051.1    Uniprot ID   A0A0H2VDV8
Organism   Escherichia coli strain Z135-5     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3549225..3560658
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABXJ20_RS17295 (ABXJ20_17295) yrbG 3549783..3550760 (+) 978 WP_000922880.1 calcium/sodium antiporter -
  ABXJ20_RS17300 (ABXJ20_17300) kdsD 3550774..3551760 (+) 987 WP_001295557.1 arabinose-5-phosphate isomerase KdsD -
  ABXJ20_RS17305 (ABXJ20_17305) kdsC 3551781..3552347 (+) 567 WP_000030016.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  ABXJ20_RS17310 (ABXJ20_17310) lptC 3552344..3552919 (+) 576 WP_000030537.1 LPS export ABC transporter periplasmic protein LptC -
  ABXJ20_RS17315 (ABXJ20_17315) lptA 3552888..3553445 (+) 558 WP_000669785.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  ABXJ20_RS17320 (ABXJ20_17320) lptB 3553452..3554177 (+) 726 WP_000224099.1 LPS export ABC transporter ATP-binding protein -
  ABXJ20_RS17325 (ABXJ20_17325) rpoN/rpoN1 3554225..3555658 (+) 1434 WP_000809051.1 RNA polymerase factor sigma-54 Machinery gene
  ABXJ20_RS17330 (ABXJ20_17330) hpf 3555681..3555968 (+) 288 WP_001176599.1 ribosome hibernation promoting factor -
  ABXJ20_RS17335 (ABXJ20_17335) ptsN 3556086..3556577 (+) 492 WP_000183674.1 PTS IIA-like nitrogen regulatory protein PtsN -
  ABXJ20_RS17340 (ABXJ20_17340) rapZ 3556623..3557477 (+) 855 WP_000243741.1 RNase adapter RapZ -
  ABXJ20_RS17345 (ABXJ20_17345) npr 3557474..3557746 (+) 273 WP_000216791.1 PTS phosphocarrier protein NPr -
  ABXJ20_RS17350 (ABXJ20_17350) yrbL 3557959..3558591 (+) 633 WP_000620405.1 PhoP regulatory network protein YrbL -
  ABXJ20_RS17355 (ABXJ20_17355) mtgA 3558588..3559316 (-) 729 WP_000047069.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  ABXJ20_RS17360 (ABXJ20_17360) elbB 3559313..3559966 (-) 654 WP_001546146.1 isoprenoid biosynthesis glyoxalase ElbB -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 53993.79 Da        Isoelectric Point: 4.3639

>NTDB_id=919086 ABXJ20_RS17325 WP_000809051.1 3554225..3555658(+) (rpoN/rpoN1) [Escherichia coli strain Z135-5]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESNPLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPE
ELPLDASWDTIYTAGTPSGTSGDYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARLIISDHLDLLANHDFRTLMRVTR
LKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDVLVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIR
SNLQDAKWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=919086 ABXJ20_RS17325 WP_000809051.1 3554225..3555658(+) (rpoN/rpoN1) [Escherichia coli strain Z135-5]
ATGAAGCAAGGTTTGCAACTCAGGCTTAGCCAACAACTGGCGATGACGCCACAGCTCCAACAGGCAATTCGTCTGTTGCA
GTTGTCGACGCTGGAACTTCAGCAAGAGCTACAGCAGGCGCTGGAGAGCAATCCGCTGCTTGAGCAAATCGACACTCATG
AAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACACTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAA
GAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGATGA
CGAGCTGCCGGTCTATCAGGGCGAAACGACGCAGACCTTACAGGATTACCTGATGTGGCAGGTCGAGTTGACACCGTTTT
CCGACACTGACCGCGCGATTGCTACCTCTATCGTCGATGCCGTTGATGACACCGGTTATCTGACTGTCCCGCTGGAAGAT
ATTCTCGAAAGTATGGGCGATGAAGAGATTGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCC
AGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGCCTGCTGATCCAACTCTCCCAATTCGATAAGACCACGCCATGGCTGG
AAGAGGCCAGACTGATCATTAGCGATCATCTCGATCTATTAGCCAATCACGACTTCCGCACTTTAATGCGCGTCACGCGT
CTGAAAGAAGATGTGCTGAAAGAAGCCGTCAATCTGATCCAGTCGCTCGATCCGCGCCCCGGGCAGTCGATCCAGACTGG
CGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGCCACTGGACGGTAGAACTCAACAGTGACAGCA
TTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAATGATGGTGATAGCCAGTTTATCCGC
AGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGTCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTAT
CGTTGAACAGCAGCAAGCCTTCTTTGAGCAAGGTGAAGAATATATGAAACCGATGGTACTGGCCGATATCGCCCAGGCTG
TCGAAATGCATGAATCGACGATATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAA
TATTTCTTTTCCAGTCACGTCAATACTGAAGGCGGGGGTGAAGCCTCTTCCACAGCGATTCGTGCGCTGGTGAAGAAATT
AATCGCGGCGGAAAACCCAGCGAAACCGTTGAGCGACAGCAAGTTAACCTCTTTGCTGTCGGAACAAGGTATCATGGTGG
CACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAGCTCGTTTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2VDV8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

44.223

100

0.465