Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   OCW38_RS01240 Genome accession   NZ_AP025480
Coordinates   279988..281013 (+) Length   341 a.a.
NCBI ID   WP_016787692.1    Uniprot ID   A0AAN0LM26
Organism   Vibrio cyclitrophicus strain LMG 21359     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 274988..286013
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OCW38_RS01225 - 275339..276067 (-) 729 WP_010435300.1 glutathione peroxidase -
  OCW38_RS01230 oxyR 276289..277182 (+) 894 WP_010435297.1 DNA-binding transcriptional regulator OxyR -
  OCW38_RS01235 - 277278..279836 (-) 2559 WP_032545551.1 penicillin-binding protein 1A -
  OCW38_RS01240 pilM 279988..281013 (+) 1026 WP_016787692.1 type IV pilus assembly protein PilM Machinery gene
  OCW38_RS01245 pilN 280997..281611 (+) 615 WP_010435288.1 PilN domain-containing protein Machinery gene
  OCW38_RS01250 pilO 281604..282233 (+) 630 WP_010435284.1 type 4a pilus biogenesis protein PilO Machinery gene
  OCW38_RS01255 pilP 282220..282738 (+) 519 WP_016787693.1 pilus assembly protein PilP Machinery gene
  OCW38_RS01260 pilQ 282798..284489 (+) 1692 WP_016787694.1 type IV pilus secretin PilQ Machinery gene
  OCW38_RS01265 aroK 284666..285184 (+) 519 WP_009848907.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 341 a.a.        Molecular weight: 38128.77 Da        Isoelectric Point: 6.8348

>NTDB_id=91905 OCW38_RS01240 WP_016787692.1 279988..281013(+) (pilM) [Vibrio cyclitrophicus strain LMG 21359]
MGSSLITGIDINHHSIKAVVLKPVGESYALVGYKELPISDDIFTANHTLEYQKTVKKLKELRKGLPFGSRNVAISVPDNT
VISKVLQIESELEDREKEFSIYQTFAHQSPFPIEELSLDFVKLEDKRFGKGSTSSYQVYATRKEVVDSRADALTKAGFKP
IVVDTQAHGLLNIWQLASHLYPEKKNWLLVDVGVDQTSLGIIPQGSAPFYKDIAFGIQDLCSTGNSMDMEGGFSNVEEKH
KFIVNLIEKLKRQLQLYSSVNALQPISGIWLMGEGASMPMVVEELERHFQLSCESLNPLSLFENKVAKKRRLPMDWQHFG
IAAGMAMSGLKWQGEKHVASN

Nucleotide


Download         Length: 1026 bp        

>NTDB_id=91905 OCW38_RS01240 WP_016787692.1 279988..281013(+) (pilM) [Vibrio cyclitrophicus strain LMG 21359]
ATGGGTTCATCATTAATTACAGGTATAGATATAAATCACCACAGCATCAAAGCCGTGGTACTAAAACCCGTTGGGGAGTC
GTATGCCCTGGTGGGATACAAAGAGCTGCCGATTTCGGACGACATTTTTACAGCTAACCATACTTTGGAGTATCAGAAAA
CTGTTAAGAAACTCAAAGAACTTAGGAAAGGGCTGCCTTTTGGAAGCCGCAACGTCGCTATTTCGGTACCGGATAACACG
GTGATCAGCAAAGTACTGCAAATAGAGAGCGAATTAGAAGATAGAGAAAAGGAGTTCTCGATCTATCAAACCTTTGCTCA
TCAATCTCCCTTTCCTATCGAGGAGCTGAGTTTAGATTTTGTAAAGCTAGAAGACAAACGATTTGGTAAAGGATCAACAA
GCAGCTATCAAGTGTATGCGACTCGTAAAGAGGTGGTTGATAGCCGAGCTGACGCGCTAACGAAAGCAGGGTTTAAGCCC
ATCGTTGTCGACACTCAGGCCCATGGTTTACTCAACATTTGGCAATTGGCTTCGCATTTGTATCCAGAAAAAAAGAATTG
GCTGTTGGTGGATGTTGGCGTTGATCAAACTTCATTAGGCATTATTCCCCAAGGTTCAGCGCCATTTTATAAAGATATTG
CCTTTGGCATTCAAGATCTTTGCAGTACGGGTAACTCGATGGATATGGAGGGTGGGTTTAGTAACGTTGAGGAGAAGCAT
AAGTTTATTGTCAATTTGATTGAAAAACTCAAGCGTCAGTTACAGCTCTATTCATCGGTCAATGCGCTTCAACCTATCTC
AGGAATTTGGCTAATGGGCGAAGGAGCAAGTATGCCGATGGTCGTTGAAGAGTTAGAGCGACATTTTCAGTTAAGTTGTG
AGTCTTTGAACCCCCTATCTTTGTTTGAGAATAAGGTCGCGAAAAAACGTCGATTACCCATGGATTGGCAACATTTTGGT
ATTGCAGCAGGCATGGCGATGAGTGGACTCAAGTGGCAGGGAGAAAAGCATGTTGCATCAAATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-220)

Type IV pilus assembly protein PilM; (PilM_2)

(242-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio cholerae strain A1552

53.392

99.413

0.531
  pilM Vibrio campbellii strain DS40M4

47.661

100

0.478

Multiple sequence alignment