Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   VNN28_RS02555 Genome accession   NZ_CP141725
Coordinates   536184..537545 (-) Length   453 a.a.
NCBI ID   WP_338998082.1    Uniprot ID   -
Organism   Lactococcus formosensis strain M03102409 EE5d7     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 531184..542545
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VNN28_RS02530 (VNN28_02530) - 531845..532339 (+) 495 WP_017368775.1 GNAT family N-acetyltransferase -
  VNN28_RS02535 (VNN28_02535) ald 532370..533491 (-) 1122 WP_165711795.1 alanine dehydrogenase -
  VNN28_RS02540 (VNN28_02540) - 533693..534199 (-) 507 WP_165711794.1 PepSY domain-containing protein -
  VNN28_RS02545 (VNN28_02545) - 534335..534964 (-) 630 WP_213495779.1 pyridoxamine 5'-phosphate oxidase family protein -
  VNN28_RS02550 (VNN28_02550) - 534973..536049 (-) 1077 WP_096368774.1 PIN/TRAM domain-containing protein -
  VNN28_RS02555 (VNN28_02555) radA 536184..537545 (-) 1362 WP_338998082.1 DNA repair protein RadA Machinery gene
  VNN28_RS02565 (VNN28_02565) - 538056..538493 (-) 438 WP_165713373.1 hypothetical protein -
  VNN28_RS02570 (VNN28_02570) - 538503..539987 (-) 1485 WP_165711791.1 S-layer homology domain-containing protein -
  VNN28_RS02575 (VNN28_02575) rplQ 540380..540760 (-) 381 WP_003133776.1 50S ribosomal protein L17 -
  VNN28_RS02580 (VNN28_02580) - 540776..541714 (-) 939 WP_003133775.1 DNA-directed RNA polymerase subunit alpha -
  VNN28_RS02585 (VNN28_02585) rpsK 541766..542149 (-) 384 WP_003133774.1 30S ribosomal protein S11 -
  VNN28_RS02590 (VNN28_02590) rpsM 542167..542532 (-) 366 WP_003133773.1 30S ribosomal protein S13 -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 49712.90 Da        Isoelectric Point: 5.1633

>NTDB_id=918984 VNN28_RS02555 WP_338998082.1 536184..537545(-) (radA) [Lactococcus formosensis strain M03102409 EE5d7]
MAKKKSTFLCQECGYKSVKKLGRCPNCGAWGSFVEEVEVQEVKNQRVSLTGQHSKPMKLDQVELFDTPRVETDLDEFNRV
LGGGVVPGSLVLIGGDPGIGKSTLLLQVSTQLASRGRVLYVSGEESAQQIKLRAERLGDIDTDFYLYAETNMQSIRNEVE
RLQPDFLIVDSIQTIMTPEIQSTQGSVSQVREVTGELMQLAKTNDIATFIVGHVTKEGQLAGPRMLEHMVDTVLYFEGER
QNTFRILRAVKNRFGSTNEIGIFEMQGSGLVEVTNPSEVFLEERLEGSTGSAIVCALEGTRPILVEIQALTTPTMFGNAK
RTTSGLDFNRVSLIMAVLEKRAGFLLQQQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKEEPTDARDCFIGEIGLTGEIR
RVTRMEQRLNEASKLGFQKVYAPKNSLAGVNTPENLKVVGVTTLSECLKKVFG

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=918984 VNN28_RS02555 WP_338998082.1 536184..537545(-) (radA) [Lactococcus formosensis strain M03102409 EE5d7]
ATAGCTAAAAAAAAATCAACATTTCTTTGTCAAGAATGTGGTTATAAATCAGTAAAAAAACTCGGACGTTGTCCCAATTG
TGGCGCTTGGGGCTCCTTTGTGGAAGAAGTAGAAGTCCAAGAGGTAAAAAATCAGAGAGTAAGTTTGACAGGTCAGCACT
CTAAACCCATGAAGTTAGATCAGGTTGAACTTTTTGATACACCGCGCGTGGAAACCGACCTAGATGAGTTTAACCGAGTC
CTTGGAGGCGGTGTTGTTCCAGGAAGTCTTGTCCTTATTGGGGGCGATCCAGGGATTGGTAAATCGACTCTACTGCTTCA
AGTATCTACACAACTTGCTTCAAGAGGGCGGGTCCTTTATGTGAGTGGGGAGGAGTCAGCTCAACAAATTAAGCTGCGTG
CTGAGCGTTTGGGCGACATTGATACAGATTTTTATCTTTATGCCGAAACCAATATGCAAAGTATCCGAAATGAAGTGGAA
CGTCTACAGCCAGATTTTCTTATTGTAGATTCGATTCAAACTATCATGACGCCTGAAATTCAGAGTACCCAGGGTTCAGT
TAGTCAAGTGCGTGAGGTGACAGGAGAATTAATGCAATTGGCCAAAACTAACGATATTGCGACTTTTATTGTGGGACATG
TTACCAAGGAAGGGCAACTTGCAGGCCCACGTATGTTGGAACATATGGTTGATACCGTCTTGTATTTTGAAGGAGAACGC
CAAAATACATTCCGAATTTTACGTGCGGTCAAAAACCGCTTTGGTTCAACAAATGAAATTGGAATTTTCGAGATGCAAGG
CAGTGGTTTAGTGGAAGTAACCAACCCGAGTGAAGTCTTTCTGGAAGAACGTCTCGAGGGTTCTACCGGATCTGCTATTG
TTTGTGCATTAGAAGGTACTCGTCCAATATTAGTTGAAATTCAAGCTTTGACTACACCAACCATGTTTGGGAATGCCAAA
CGTACAACCTCCGGTTTAGACTTTAACCGTGTGAGTTTAATCATGGCAGTACTTGAAAAGCGGGCCGGATTTCTTTTGCA
GCAACAAGACGCTTACTTGAAGTCAGCAGGTGGCGTTAAATTGGATGAGCCTGCAATTGACTTAGCAGTTGCAGTAGCTA
TTGCTTCTAGCTACAAAGAAGAGCCAACAGATGCACGCGATTGCTTTATTGGTGAAATTGGCTTAACTGGTGAAATCCGT
CGTGTCACACGCATGGAGCAACGTCTTAATGAAGCGAGTAAATTAGGTTTCCAAAAAGTTTATGCGCCTAAGAATAGCTT
AGCAGGCGTAAATACTCCAGAAAACTTGAAAGTGGTTGGTGTGACAACTTTATCTGAATGTTTGAAAAAAGTATTTGGTT
AA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(77-218)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(359-425)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis NCTC 12261

79.912

100

0.799
  radA Streptococcus pneumoniae Rx1

79.912

100

0.799
  radA Streptococcus pneumoniae D39

79.912

100

0.799
  radA Streptococcus pneumoniae R6

79.912

100

0.799
  radA Streptococcus pneumoniae TIGR4

79.912

100

0.799
  radA Streptococcus mitis SK321

79.912

100

0.799
  radA Bacillus subtilis subsp. subtilis str. 168

61.438

100

0.623