Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   VNN27_RS02615 Genome accession   NZ_CP141700
Coordinates   553914..555275 (-) Length   453 a.a.
NCBI ID   WP_346350225.1    Uniprot ID   -
Organism   Lactococcus petauri strain R22-25 IC35     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 548914..560275
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VNN27_RS02595 (VNN27_02595) - 550221..550715 (+) 495 WP_019291419.1 GNAT family N-acetyltransferase -
  VNN27_RS02600 (VNN27_02600) ald 550746..551867 (-) 1122 WP_019291420.1 alanine dehydrogenase -
  VNN27_RS02605 (VNN27_02605) - 552054..552683 (-) 630 WP_019291421.1 pyridoxamine 5'-phosphate oxidase family protein -
  VNN27_RS02610 (VNN27_02610) - 552706..553782 (-) 1077 WP_019291422.1 PIN/TRAM domain-containing protein -
  VNN27_RS02615 (VNN27_02615) radA 553914..555275 (-) 1362 WP_346350225.1 DNA repair protein RadA Machinery gene
  VNN27_RS02625 (VNN27_02625) - 555784..556236 (-) 453 WP_019292998.1 hypothetical protein -
  VNN27_RS02630 (VNN27_02630) - 556246..557730 (-) 1485 WP_346350124.1 S-layer homology domain-containing protein -
  VNN27_RS02635 (VNN27_02635) rplQ 558119..558499 (-) 381 WP_003133776.1 50S ribosomal protein L17 -
  VNN27_RS02640 (VNN27_02640) - 558515..559453 (-) 939 WP_003133775.1 DNA-directed RNA polymerase subunit alpha -
  VNN27_RS02645 (VNN27_02645) rpsK 559505..559888 (-) 384 WP_003133774.1 30S ribosomal protein S11 -
  VNN27_RS02650 (VNN27_02650) rpsM 559906..560271 (-) 366 WP_003133773.1 30S ribosomal protein S13 -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 49754.94 Da        Isoelectric Point: 4.9267

>NTDB_id=918489 VNN27_RS02615 WP_346350225.1 553914..555275(-) (radA) [Lactococcus petauri strain R22-25 IC35]
MAKKKSTFLCQECGYKSVKKLGRCPNCGAWGSFVEEVEVQEVKNQRVSLTGEHSKPMKLDQVELFETPRVETDLDEFNRV
LGGGVVPGSLVLIGGDPGIGKSTLLLQVSTQLASRGRVLYVSGEESAQQIKLRAERLGDIDTDFYLYAETNMQSIRNEVE
RLQPDFLIVDSIQTIMTPEIQSTQGSVSQVREVTGELMQLAKTNDIATFIVGHVTKEGQLAGPRMLEHMVDTVLYFEGER
QNTFRILRAVKNRFGSTNEIGIFEMQGSGLVEVTNPSEVFLEERLEGSTGSAIVCALEGTRPILVEIQALTTPTMFGNAK
RTTSGLDFNRVSLIMAVLEKRAGFLLQQQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKEEPTDARECFIGEIGLTGEIR
RVTRMEQRLNEASKLGFQKVYAPKNSLAGVDIPENLQVIGVTTLSECVKKVFG

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=918489 VNN27_RS02615 WP_346350225.1 553914..555275(-) (radA) [Lactococcus petauri strain R22-25 IC35]
ATAGCTAAAAAAAAATCAACCTTTCTTTGTCAAGAATGTGGCTATAAATCAGTAAAAAAACTCGGACGTTGTCCCAATTG
TGGCGCTTGGGGCTCCTTTGTAGAAGAAGTAGAAGTCCAAGAGGTAAAAAATCAGAGAGTAAGTTTGACAGGTGAGCACT
CTAAACCCATGAAGTTAGATCAGGTTGAACTTTTTGAAACACCGCGCGTGGAAACCGACCTAGATGAGTTTAACCGAGTC
CTTGGGGGCGGTGTCGTTCCAGGAAGTCTTGTCCTTATTGGGGGCGATCCAGGGATTGGTAAATCAACTCTACTGCTTCA
AGTATCTACACAACTTGCTTCAAGAGGACGTGTCCTTTATGTGAGTGGGGAGGAATCAGCTCAACAAATTAAGCTGCGTG
CTGAGCGTTTGGGCGACATTGATACAGATTTTTATCTGTATGCCGAAACCAATATGCAAAGTATCCGAAATGAAGTGGAA
CGTCTACAGCCAGATTTTCTTATTGTAGATTCGATTCAAACTATCATGACGCCTGAAATTCAGAGTACCCAGGGTTCAGT
TAGTCAAGTGCGTGAGGTGACAGGAGAATTAATGCAGTTGGCCAAAACCAACGATATTGCGACTTTTATTGTCGGACATG
TCACCAAGGAAGGGCAACTTGCAGGCCCACGTATGTTGGAACATATGGTTGATACCGTCTTGTATTTTGAGGGAGAACGC
CAAAATACATTCCGAATTTTACGCGCGGTCAAAAACCGTTTTGGTTCAACAAATGAAATTGGAATTTTCGAGATGCAAGG
CAGTGGTTTAGTGGAAGTAACCAACCCGAGTGAAGTCTTTCTGGAAGAACGTCTAGAGGGTTCTACCGGATCTGCTATCG
TCTGTGCGTTAGAAGGTACTCGTCCGATATTGGTTGAAATTCAAGCTTTGACAACACCAACCATGTTTGGGAATGCCAAA
CGTACAACCTCCGGTTTAGACTTTAACCGTGTGAGTTTAATCATGGCAGTACTTGAAAAGCGTGCCGGTTTTCTTTTACA
ACAGCAAGATGCTTACTTGAAATCTGCAGGTGGGGTCAAGTTGGATGAACCAGCCATCGACTTGGCAGTCGCTGTGGCAA
TTGCTTCCAGCTATAAGGAAGAGCCAACAGATGCACGTGAATGCTTTATTGGCGAAATTGGCCTAACTGGAGAAATCCGA
CGTGTCACACGTATGGAGCAACGTCTCAATGAAGCAAGTAAATTAGGTTTCCAAAAAGTATATGCGCCTAAGAATAGCTT
AGCAGGTGTAGATATTCCAGAAAATTTGCAAGTGATCGGCGTGACGACTTTATCTGAATGTGTGAAAAAAGTGTTTGGCT
AA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(79-218)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(359-425)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae Rx1

80.574

100

0.806
  radA Streptococcus pneumoniae D39

80.574

100

0.806
  radA Streptococcus pneumoniae R6

80.574

100

0.806
  radA Streptococcus pneumoniae TIGR4

80.574

100

0.806
  radA Streptococcus mitis NCTC 12261

80.574

100

0.806
  radA Streptococcus mitis SK321

80.574

100

0.806
  radA Bacillus subtilis subsp. subtilis str. 168

61.438

100

0.623