Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   VNN34_RS02925 Genome accession   NZ_CP141696
Coordinates   598988..600349 (-) Length   453 a.a.
NCBI ID   WP_029345652.1    Uniprot ID   A0A252CD69
Organism   Lactococcus petauri strain S69-A     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 593988..605349
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VNN34_RS02905 (VNN34_02905) - 595295..595789 (+) 495 WP_019291419.1 GNAT family N-acetyltransferase -
  VNN34_RS02910 (VNN34_02910) ald 595820..596941 (-) 1122 WP_086582767.1 alanine dehydrogenase -
  VNN34_RS02915 (VNN34_02915) - 597128..597757 (-) 630 WP_019292996.1 pyridoxamine 5'-phosphate oxidase family protein -
  VNN34_RS02920 (VNN34_02920) - 597780..598856 (-) 1077 WP_086582768.1 PIN/TRAM domain-containing protein -
  VNN34_RS02925 (VNN34_02925) radA 598988..600349 (-) 1362 WP_029345652.1 DNA repair protein RadA Machinery gene
  VNN34_RS02935 (VNN34_02935) - 600858..601310 (-) 453 WP_019335985.1 hypothetical protein -
  VNN34_RS02940 (VNN34_02940) - 601320..602804 (-) 1485 WP_019335986.1 S-layer homology domain-containing protein -
  VNN34_RS02945 (VNN34_02945) rplQ 603193..603573 (-) 381 WP_003133776.1 50S ribosomal protein L17 -
  VNN34_RS02950 (VNN34_02950) - 603589..604527 (-) 939 WP_003133775.1 DNA-directed RNA polymerase subunit alpha -
  VNN34_RS02955 (VNN34_02955) rpsK 604579..604962 (-) 384 WP_003133774.1 30S ribosomal protein S11 -
  VNN34_RS02960 (VNN34_02960) rpsM 604980..605345 (-) 366 WP_003133773.1 30S ribosomal protein S13 -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 49754.94 Da        Isoelectric Point: 4.9237

>NTDB_id=918308 VNN34_RS02925 WP_029345652.1 598988..600349(-) (radA) [Lactococcus petauri strain S69-A]
MAKKKSTFLCQECGYKSVKKLGRCPNCGAWGSFVEEVEVQEVKNQRVSLTGEHSKPMKLDQVELFDTPRVETDLDEFNRV
LGGGVVPGSLVLIGGDPGIGKSTLLLQVSTQLASRGRVLYVSGEESAQQIKLRAERLGDIDTDFYLYAETNMQSIRNEVE
RLQPDFLIVDSIQTIMTPEIQSTQGSVSQVREVTGELMQLAKTNDIATFIVGHVTKEGQLAGPRMLEHMVDTVLYFEGER
QNTFRILRAVKNRFGSTNEIGIFEMQGSGLVEVTNPSEVFLEERLEGSTGSAIVCALEGTRPILVEIQALTTPTMFGNAK
RTTSGLDFNRVSLIMAVLEKRAGFLLQQQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKEEPTDARECFIGEIGLTGEIR
RVTRMEQRLNEASKLGFQKVYAPKNSLAGVDIPENLQVIGVTTLSECLKKVFG

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=918308 VNN34_RS02925 WP_029345652.1 598988..600349(-) (radA) [Lactococcus petauri strain S69-A]
ATAGCTAAAAAAAAATCAACCTTTCTTTGTCAAGAATGTGGCTATAAATCAGTAAAAAAACTCGGACGTTGTCCCAATTG
TGGCGCTTGGGGCTCCTTTGTGGAAGAAGTAGAAGTCCAAGAGGTAAAAAATCAGAGAGTAAGTTTGACAGGTGAGCACT
CTAAACCCATGAAGTTAGATCAGGTTGAACTTTTTGATACACCGCGCGTGGAAACCGACCTAGATGAGTTTAACCGAGTC
CTTGGAGGCGGTGTCGTTCCAGGAAGTCTTGTCCTTATTGGGGGCGATCCAGGGATTGGTAAATCGACTCTACTGCTTCA
AGTATCTACACAACTTGCTTCAAGAGGGCGTGTGCTTTATGTGAGTGGGGAGGAGTCAGCTCAACAAATTAAGCTGCGTG
CTGAGCGTTTGGGCGACATTGATACAGATTTTTATCTTTATGCCGAAACCAATATGCAAAGTATCCGAAATGAAGTGGAA
CGTCTACAGCCAGATTTTCTTATTGTAGATTCGATTCAAACTATCATGACGCCTGAAATTCAGAGTACCCAAGGTTCAGT
TAGTCAAGTGCGCGAGGTGACAGGAGAATTAATGCAGTTGGCCAAAACTAACGATATTGCGACTTTTATTGTCGGACATG
TCACCAAGGAAGGGCAACTTGCAGGCCCACGTATGTTGGAACATATGGTTGATACCGTCTTGTATTTTGAAGGAGAACGC
CAAAATACATTCCGAATTTTACGCGCGGTCAAAAACCGTTTTGGTTCAACAAATGAAATTGGAATTTTCGAGATGCAAGG
CAGTGGTTTAGTGGAAGTAACCAACCCGAGTGAAGTCTTTCTGGAAGAACGTCTAGAGGGTTCTACCGGATCTGCTATCG
TCTGTGCGTTAGAAGGTACTCGTCCGATATTGGTTGAAATTCAAGCTTTGACAACACCAACCATGTTTGGGAATGCCAAA
CGTACAACCTCCGGTTTAGACTTTAACCGTGTGAGTTTAATCATGGCAGTACTTGAAAAGCGTGCCGGTTTTCTTTTACA
ACAGCAAGATGCTTACTTGAAATCTGCAGGTGGGGTCAAGTTGGATGAACCAGCCATCGACTTGGCAGTCGCTGTGGCAA
TTGCTTCCAGCTATAAGGAAGAGCCAACAGATGCACGTGAATGCTTTATTGGCGAAATTGGCCTAACTGGAGAAATCCGA
CGTGTCACACGTATGGAGCAACGTCTCAATGAAGCAAGTAAATTAGGTTTCCAAAAAGTATATGCGCCTAAGAATAGCTT
AGCAGGTGTAGATATTCCAGAAAATTTGCAAGTGATCGGCGTGACGACTTTATCTGAATGTTTGAAAAAAGTGTTTGGCT
AA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(77-218)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(359-425)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A252CD69

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae Rx1

80.795

100

0.808
  radA Streptococcus pneumoniae D39

80.795

100

0.808
  radA Streptococcus pneumoniae R6

80.795

100

0.808
  radA Streptococcus pneumoniae TIGR4

80.795

100

0.808
  radA Streptococcus mitis NCTC 12261

80.795

100

0.808
  radA Streptococcus mitis SK321

80.795

100

0.808
  radA Bacillus subtilis subsp. subtilis str. 168

61.438

100

0.623