Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   AAFM42_RS15285 Genome accession   NZ_CP160329
Coordinates   3044517..3046010 (-) Length   497 a.a.
NCBI ID   WP_053254420.1    Uniprot ID   A0ABY1TCC4
Organism   Pseudomonas fluorescens strain PFX1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3039517..3051010
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAFM42_RS15250 (AAFM42_15200) - 3040444..3040872 (+) 429 WP_053254415.1 hypothetical protein -
  AAFM42_RS15255 (AAFM42_15205) - 3040882..3041493 (+) 612 WP_053254416.1 superoxide dismutase -
  AAFM42_RS15260 (AAFM42_15210) - 3041516..3042409 (+) 894 WP_053254417.1 ZIP family metal transporter -
  AAFM42_RS15265 (AAFM42_15215) - 3042460..3042732 (-) 273 WP_049709983.1 HPr family phosphocarrier protein -
  AAFM42_RS15270 (AAFM42_15220) rapZ 3042761..3043618 (-) 858 WP_053254418.1 RNase adapter RapZ -
  AAFM42_RS15275 (AAFM42_15225) ptsN 3043635..3044099 (-) 465 WP_053254419.1 PTS IIA-like nitrogen regulatory protein PtsN -
  AAFM42_RS15280 (AAFM42_15230) hpf 3044162..3044467 (-) 306 WP_394546102.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  AAFM42_RS15285 (AAFM42_15235) rpoN/rpoN1 3044517..3046010 (-) 1494 WP_053254420.1 RNA polymerase factor sigma-54 Machinery gene
  AAFM42_RS15290 (AAFM42_15240) lptB 3046021..3046746 (-) 726 WP_053254421.1 LPS export ABC transporter ATP-binding protein -
  AAFM42_RS15295 (AAFM42_15245) lptA 3046746..3047321 (-) 576 WP_053254422.1 lipopolysaccharide transport periplasmic protein LptA -
  AAFM42_RS15300 (AAFM42_15250) lptC 3047308..3047880 (-) 573 WP_053254423.1 LPS export ABC transporter periplasmic protein LptC -
  AAFM42_RS15305 (AAFM42_15255) - 3047896..3048420 (-) 525 WP_053254424.1 HAD family hydrolase -
  AAFM42_RS15310 (AAFM42_15260) - 3048421..3049395 (-) 975 WP_053254425.1 KpsF/GutQ family sugar-phosphate isomerase -
  AAFM42_RS15315 (AAFM42_15265) - 3049466..3050275 (+) 810 WP_053254426.1 ATP-binding cassette domain-containing protein -

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 56640.78 Da        Isoelectric Point: 4.3925

>NTDB_id=917730 AAFM42_RS15285 WP_053254420.1 3044517..3046010(-) (rpoN/rpoN1) [Pseudomonas fluorescens strain PFX1]
MKPSLVLRMGQQLTMTPQLQQAIRLLQLSTLDLQQEIQEALESNPMLERQEEGDDFDNADPLADNIEQKPNSDVQEPSYQ
ETAPTVDNLEEGEWNERIPNELPVDTAWEDVYQTSASSLPSNDDDEWDFTTRTSVGESLQSHLLWQLNLAPMSDTDRLIA
VTLIDCINNQGYLDETLEEILEAFDPELDIELDEIEAVLHRIQQFEPAGIGARTLSECLLLQLRQLPAKTLWLAEAQRLV
TDYIDLLGSRDYSQLMRRMKLKEDDLRQVIELVQSLNPRPGSQIESSEAEYVVPDVIVRKDNERWLVELNQESVPRLRVN
PQYAGFVRRADTSADNTFMRNQLQEARWFIKSLQSRNETLMKVATQIVEHQRGFLEYGDEAMKPLVLHDIAEAVGMHEST
ISRVTTQKFMHTPRGIYELKYFFSSHVSTSEGGECSSTAIRAIIKKLVAAENQKKPLSDSKIAGLLEAQGIQVARRTVAK
YRESLGIAPSSERKRLM

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=917730 AAFM42_RS15285 WP_053254420.1 3044517..3046010(-) (rpoN/rpoN1) [Pseudomonas fluorescens strain PFX1]
ATGAAACCATCGCTAGTCCTGAGAATGGGCCAGCAGCTGACGATGACACCGCAGCTGCAACAGGCCATCCGCCTGCTCCA
ATTGTCGACCCTGGACCTGCAACAGGAAATCCAGGAGGCCCTGGAGTCCAATCCGATGCTCGAACGCCAGGAAGAAGGCG
ACGACTTCGATAATGCAGACCCGCTGGCCGACAACATCGAGCAAAAACCCAACTCCGACGTGCAGGAACCTTCCTATCAG
GAAACCGCCCCTACGGTGGACAACCTTGAGGAAGGCGAATGGAACGAACGCATTCCCAACGAGCTTCCGGTGGACACCGC
CTGGGAAGACGTTTACCAGACCAGCGCCAGCAGCCTGCCCAGCAATGATGATGACGAGTGGGACTTCACCACCCGCACCT
CCGTCGGCGAAAGCCTGCAGAGCCATTTGCTGTGGCAATTGAACCTGGCGCCGATGTCCGACACCGATCGCCTGATCGCC
GTGACCCTGATCGACTGCATCAATAACCAGGGTTACCTCGACGAGACGCTCGAAGAGATCCTTGAGGCTTTCGATCCGGA
ACTGGACATCGAACTGGACGAAATCGAAGCCGTCCTGCACCGCATCCAGCAGTTCGAGCCCGCCGGCATCGGCGCCCGCA
CACTGAGCGAGTGCCTGCTGCTGCAACTGCGCCAGCTGCCCGCCAAGACCCTGTGGCTCGCCGAAGCCCAGCGCCTGGTC
ACCGACTACATCGACCTGCTGGGCAGCCGCGATTACAGCCAGCTGATGCGCCGCATGAAGCTCAAGGAAGACGACCTGCG
CCAGGTCATCGAGCTGGTACAGAGCCTTAACCCCCGCCCGGGCTCGCAGATCGAGTCCAGCGAAGCCGAATACGTCGTTC
CCGATGTAATCGTGCGCAAAGACAACGAGCGTTGGCTGGTGGAACTGAACCAGGAGTCGGTGCCTCGCCTGCGCGTCAAC
CCGCAATACGCAGGTTTTGTGCGCCGTGCCGACACCAGTGCCGATAACACCTTCATGCGCAATCAGTTGCAGGAAGCGCG
CTGGTTCATCAAGAGCCTGCAAAGCCGCAACGAAACCCTGATGAAAGTGGCCACTCAGATCGTCGAGCACCAGCGCGGCT
TCCTCGAATATGGTGACGAGGCCATGAAACCCTTGGTCCTGCACGATATCGCTGAGGCGGTTGGCATGCATGAATCGACG
ATTTCCCGGGTAACCACGCAGAAATTCATGCATACCCCACGGGGTATTTATGAGCTGAAATACTTTTTCTCCAGCCACGT
AAGTACCTCCGAAGGCGGCGAATGCTCGTCCACGGCGATCCGCGCGATCATTAAAAAACTGGTTGCCGCGGAAAATCAGA
AAAAGCCGTTGAGTGACAGCAAGATCGCTGGTTTACTGGAGGCACAAGGCATTCAGGTCGCCCGTCGAACCGTCGCCAAG
TACCGCGAGTCCCTCGGGATCGCGCCTTCCAGCGAGCGTAAGCGTTTGATGTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(337-495)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(131-323)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

44.554

100

0.453