Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   AB0U13_RS21650 Genome accession   NZ_CP160116
Coordinates   4403759..4405192 (+) Length   477 a.a.
NCBI ID   WP_000809051.1    Uniprot ID   A0A0H2VDV8
Organism   Escherichia coli strain 729_xx_1_3_yolk     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4398759..4410192
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB0U13_RS21620 yrbG 4399317..4400294 (+) 978 WP_000922880.1 calcium/sodium antiporter -
  AB0U13_RS21625 kdsD 4400308..4401294 (+) 987 WP_001295557.1 arabinose-5-phosphate isomerase KdsD -
  AB0U13_RS21630 kdsC 4401315..4401881 (+) 567 WP_000030016.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  AB0U13_RS21635 lptC 4401878..4402453 (+) 576 WP_000030537.1 LPS export ABC transporter periplasmic protein LptC -
  AB0U13_RS21640 lptA 4402422..4402979 (+) 558 WP_000669785.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  AB0U13_RS21645 lptB 4402986..4403711 (+) 726 WP_000224099.1 LPS export ABC transporter ATP-binding protein -
  AB0U13_RS21650 rpoN/rpoN1 4403759..4405192 (+) 1434 WP_000809051.1 RNA polymerase factor sigma-54 Machinery gene
  AB0U13_RS21655 hpf 4405215..4405502 (+) 288 WP_001176599.1 ribosome hibernation promoting factor -
  AB0U13_RS21660 ptsN 4405620..4406111 (+) 492 WP_000183674.1 PTS IIA-like nitrogen regulatory protein PtsN -
  AB0U13_RS21665 rapZ 4406157..4407011 (+) 855 WP_000243741.1 RNase adapter RapZ -
  AB0U13_RS21670 npr 4407008..4407280 (+) 273 WP_000216791.1 PTS phosphocarrier protein NPr -
  AB0U13_RS21675 yrbL 4407494..4408126 (+) 633 WP_000620407.1 PhoP regulatory network protein YrbL -
  AB0U13_RS21680 mtgA 4408123..4408851 (-) 729 WP_000047069.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  AB0U13_RS21685 elbB 4408848..4409501 (-) 654 WP_000719794.1 isoprenoid biosynthesis glyoxalase ElbB -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 53993.79 Da        Isoelectric Point: 4.3639

>NTDB_id=917169 AB0U13_RS21650 WP_000809051.1 4403759..4405192(+) (rpoN/rpoN1) [Escherichia coli strain 729_xx_1_3_yolk]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESNPLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPE
ELPLDASWDTIYTAGTPSGTSGDYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARLIISDHLDLLANHDFRTLMRVTR
LKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDVLVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIR
SNLQDAKWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=917169 AB0U13_RS21650 WP_000809051.1 4403759..4405192(+) (rpoN/rpoN1) [Escherichia coli strain 729_xx_1_3_yolk]
ATGAAGCAAGGTTTGCAACTCAGGCTTAGCCAACAACTGGCGATGACGCCACAGCTCCAACAGGCAATTCGTCTGTTGCA
GTTGTCGACGCTGGAACTTCAGCAGGAGCTACAGCAGGCGCTGGAGAGCAATCCGCTGCTTGAGCAAATCGACACTCATG
AAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACACTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAA
GAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGA
CGAGCTGCCGGTCTATCAGGGCGAAACGACACAGACCTTACAGGATTACCTGATGTGGCAGGTCGAGTTGACACCATTTT
CCGACACTGACCGCGCGATTGCTACCTCTATCGTCGATGCCGTTGATGACACCGGTTATCTGACTGTCCCGCTGGAAGAT
ATTCTCGAAAGTATGGGCGATGAAGAGATTGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCC
GGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGCCTGCTGATCCAACTCTCCCAATTCGATAAGACCACGCCATGGCTGG
AAGAGGCCAGACTGATCATTAGCGATCATCTCGATCTATTAGCCAATCACGACTTCCGCACTTTAATGCGCGTCACGCGT
CTGAAAGAAGATGTGCTGAAAGAAGCCGTCAATCTGATCCAGTCGCTCGATCCGCGCCCCGGGCAGTCGATCCAGACTGG
CGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAACTCAACAGTGACAGCA
TTCCGCGTTTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGATAGCCAGTTTATCCGC
AGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGTCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTAT
CGTTGAACAGCAGCAAGCCTTCTTTGAGCAAGGCGAAGAATATATGAAACCGATGGTACTGGCTGATATCGCCCAGGCCG
TCGAGATGCATGAATCGACGATATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAG
TATTTCTTTTCCAGTCACGTCAATACTGAAGGCGGGGGTGAAGCCTCTTCCACAGCGATTCGCGCACTGGTGAAGAAATT
AATCGCGGCGGAAAACCCAGCGAAACCGTTGAGCGACAGCAAGTTAACCTCTTTGCTGTCGGAACAAGGTATCATGGTGG
CACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAGCTCGTTTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2VDV8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

44.223

100

0.465