Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   U4959_RS02700 Genome accession   NZ_CP141284
Coordinates   568801..569601 (+) Length   266 a.a.
NCBI ID   WP_151690577.1    Uniprot ID   -
Organism   Acinetobacter junii strain H1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 563801..574601
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U4959_RS02675 (U4959_02675) - 564711..566027 (+) 1317 WP_171493065.1 glycosyltransferase family 4 protein -
  U4959_RS02680 (U4959_02680) - 566055..566606 (+) 552 WP_151690576.1 phosphatase PAP2 family protein -
  U4959_RS02685 (U4959_02685) iscX 566689..566889 (+) 201 WP_004652532.1 Fe-S cluster assembly protein IscX -
  U4959_RS02690 (U4959_02690) ndk 566996..567427 (+) 432 WP_003654870.1 nucleoside-diphosphate kinase -
  U4959_RS02695 (U4959_02695) rlmN 567549..568784 (+) 1236 WP_042891255.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  U4959_RS02700 (U4959_02700) pilF 568801..569601 (+) 801 WP_151690577.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  U4959_RS02705 (U4959_02705) - 569592..570383 (+) 792 WP_151690578.1 helix-turn-helix domain-containing protein -
  U4959_RS02710 (U4959_02710) ispG 570408..571523 (+) 1116 WP_004908985.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  U4959_RS02715 (U4959_02715) hisS 571520..572812 (+) 1293 WP_151690579.1 histidine--tRNA ligase -
  U4959_RS02720 (U4959_02720) - 572844..573542 (+) 699 WP_004950316.1 YfgM family protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30352.42 Da        Isoelectric Point: 9.5600

>NTDB_id=916880 U4959_RS02700 WP_151690577.1 568801..569601(+) (pilF) [Acinetobacter junii strain H1]
MNTTRLKTILATTVVFTVLGLTACQSTTLRKDPEKAVQVRTQLAAEYIRTRDLDSAKRSLDQALKIDPRDATANMMMGVL
LQQEGSKPNMDKAEAYFKRAISSEPDNAQARNNYGTYLYQMQRYNEAIEQFTVAGATLGYEQRYQSLENLGRIYLKLGDV
ANAEKSFKQALQANRNSTISMLELSEIFYLQQNNRDASQLYEQYVRNVGQKNQGARALWIGIRIARANADQLGMQVLVNQ
MRALFPDSPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=916880 U4959_RS02700 WP_151690577.1 568801..569601(+) (pilF) [Acinetobacter junii strain H1]
TTGAATACAACTCGACTAAAAACGATTTTAGCTACTACTGTTGTGTTTACTGTATTAGGTTTGACTGCCTGCCAATCAAC
AACGTTAAGAAAAGATCCAGAAAAGGCAGTACAAGTCCGAACTCAACTCGCTGCTGAATATATTCGAACACGTGATTTGG
ATTCAGCGAAACGTTCCCTTGATCAAGCTTTAAAAATTGATCCACGTGATGCTACTGCCAACATGATGATGGGTGTTTTG
TTGCAACAAGAAGGCAGCAAACCAAACATGGACAAAGCAGAAGCTTATTTTAAGCGAGCAATCTCTTCAGAACCCGATAA
TGCCCAAGCTCGTAATAATTACGGTACATATTTGTATCAAATGCAACGTTATAATGAGGCGATTGAGCAATTTACCGTTG
CAGGTGCGACGTTAGGTTACGAACAGCGTTACCAATCGTTGGAAAACTTGGGTCGAATCTATTTAAAACTGGGTGATGTT
GCGAATGCTGAAAAATCATTCAAGCAAGCCTTACAAGCAAATCGAAATTCGACAATTTCAATGTTAGAGTTGTCCGAAAT
TTTCTATTTGCAACAAAATAATAGAGATGCATCGCAACTTTATGAACAATACGTTCGTAATGTCGGGCAGAAAAATCAAG
GTGCTCGTGCACTTTGGATCGGTATACGCATTGCTCGAGCGAATGCGGATCAATTAGGAATGCAAGTATTAGTGAATCAA
ATGCGTGCTTTATTTCCTGATAGTCCAGAATATCAACGTTATTTGCAATTACAGTACAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_16)

(89-134)

Tetratricopeptide repeat (TPR_8)

(39-69)

Tetratricopeptide repeat (TPR_8)

(145-176)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

78.652

100

0.789
  pilF Acinetobacter baylyi ADP1

67.669

100

0.677