Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   U7118_RS21865 Genome accession   NZ_CP141283
Coordinates   4065586..4066962 (-) Length   458 a.a.
NCBI ID   WP_014475593.1    Uniprot ID   -
Organism   Bacillus subtilis strain DKU_09     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4060586..4071962
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U7118_RS21845 (U7118_21845) ispF 4062102..4062578 (-) 477 WP_003225745.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  U7118_RS21850 (U7118_21850) ispD 4062571..4063269 (-) 699 WP_014478616.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  U7118_RS21855 (U7118_21855) yacL 4063284..4064384 (-) 1101 WP_003235014.1 PIN/TRAM domain-containing protein -
  U7118_RS21860 (U7118_21860) disA 4064500..4065582 (-) 1083 WP_003225736.1 DNA integrity scanning diadenylate cyclase DisA -
  U7118_RS21865 (U7118_21865) radA 4065586..4066962 (-) 1377 WP_014475593.1 DNA repair protein RadA Machinery gene
  U7118_RS21870 (U7118_21870) clpC 4067054..4069486 (-) 2433 WP_014475592.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  U7118_RS21875 (U7118_21875) mcsB 4069483..4070574 (-) 1092 WP_003235007.1 protein arginine kinase -
  U7118_RS21880 (U7118_21880) mcsA 4070574..4071131 (-) 558 WP_015252993.1 protein-arginine kinase activator protein McsA -
  U7118_RS21885 (U7118_21885) ctsR 4071145..4071609 (-) 465 WP_003225724.1 transcriptional regulator CtsR -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 49466.99 Da        Isoelectric Point: 8.2399

>NTDB_id=916860 U7118_RS21865 WP_014475593.1 4065586..4066962(-) (radA) [Bacillus subtilis strain DKU_09]
MAKTKSKFICQSCGYESPKWMGKCPGCGAWNTMVEEMIKKAPANRRAAFSHSVQTVQKPSPITSIETSEEPRVKTQLGEF
NRVLGGGVVKGSLVLIGGDPGIGKSTLLLQVSAQLSGSSNSVLYISGEESVKQTKLRADRLGINNPSLHVLSETDMEYIS
SAIQEMNPAFVVVDSIQTVYQSDITSAPGSVSQVRECTAELMKIAKTKGIPIFIVGHVTKEGSIAGPRLLEHMVDTVLYF
EGERHHTFRILRAVKNRFGSTNEMGIFEMREEGLTEVLNPSEIFLEERSAGSAGSSITASMEGTRPILVEIQALISPTSF
GNPRRMATGIDHNRVSLLMAVLEKRVGLLLQNQDAYLKVAGGVKLDEPAIDLAIVISIASSFRDTPPNPADCFIGEVGLT
GEVRRVSRIEQRVKEAAKLGFKRMIIPAANLDGWTKPKGIEVIGVANVAEALRTSLGG

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=916860 U7118_RS21865 WP_014475593.1 4065586..4066962(-) (radA) [Bacillus subtilis strain DKU_09]
ATGGCTAAAACAAAATCGAAATTCATCTGCCAATCCTGCGGCTACGAGTCTCCAAAATGGATGGGGAAATGTCCGGGCTG
CGGTGCTTGGAATACAATGGTGGAAGAAATGATTAAAAAAGCACCGGCCAATCGGAGAGCGGCTTTTTCTCATTCTGTTC
AAACTGTACAGAAACCTTCACCTATTACATCAATCGAAACATCAGAAGAACCCCGCGTCAAAACCCAGCTTGGCGAGTTT
AACAGAGTACTCGGCGGAGGTGTCGTTAAAGGCTCCCTCGTTTTAATTGGCGGTGATCCTGGTATCGGAAAGTCAACGCT
ATTACTGCAGGTTTCCGCTCAATTATCAGGCTCATCAAACAGTGTCCTGTATATTTCGGGAGAAGAATCTGTAAAGCAAA
CGAAGCTGCGAGCAGACCGTCTGGGCATTAATAATCCGTCACTGCATGTTTTATCTGAAACCGATATGGAGTATATTTCG
TCTGCTATACAAGAGATGAATCCAGCGTTTGTCGTTGTTGACTCTATTCAAACGGTTTACCAAAGCGATATTACATCGGC
TCCAGGCAGTGTGTCACAGGTCAGAGAATGTACTGCTGAGCTGATGAAAATCGCAAAAACAAAAGGTATTCCGATTTTTA
TCGTAGGGCACGTGACGAAAGAAGGGTCTATTGCAGGTCCGAGACTGTTGGAGCATATGGTTGACACTGTTTTATATTTT
GAAGGAGAACGCCACCATACTTTCCGTATTTTGCGGGCTGTAAAAAACCGTTTCGGATCTACAAACGAAATGGGCATTTT
CGAAATGCGGGAAGAGGGGCTCACTGAGGTTTTGAATCCTTCGGAAATTTTCTTAGAAGAACGCTCTGCTGGTTCCGCAG
GCTCGAGTATCACTGCCTCTATGGAGGGCACTAGACCGATTCTCGTTGAAATTCAGGCGCTCATCTCGCCAACAAGCTTT
GGCAACCCAAGGCGTATGGCAACGGGAATAGACCATAACAGGGTTTCTCTGTTAATGGCTGTGTTAGAAAAAAGAGTTGG
ACTGCTGCTGCAAAATCAGGATGCTTATTTGAAAGTGGCTGGCGGCGTTAAGCTTGATGAACCGGCAATTGATCTTGCGA
TTGTGATTAGTATCGCATCAAGCTTTAGAGACACACCTCCAAATCCTGCAGATTGTTTTATTGGAGAAGTGGGATTAACC
GGAGAAGTCCGGCGGGTTTCAAGAATTGAACAGCGTGTGAAAGAAGCGGCAAAGCTTGGCTTTAAACGCATGATCATACC
CGCGGCAAATCTGGACGGATGGACAAAACCAAAAGGGATTGAGGTTATCGGAGTAGCAAATGTTGCAGAAGCGCTTCGTA
CTTCATTAGGAGGATAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(365-430)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(72-222)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

99.782

100

0.998
  radA Streptococcus pneumoniae Rx1

63.135

98.908

0.624
  radA Streptococcus pneumoniae D39

63.135

98.908

0.624
  radA Streptococcus pneumoniae R6

63.135

98.908

0.624
  radA Streptococcus pneumoniae TIGR4

63.135

98.908

0.624
  radA Streptococcus mitis NCTC 12261

63.135

98.908

0.624
  radA Streptococcus mitis SK321

62.914

98.908

0.622