Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   U7118_RS20525 Genome accession   NZ_CP141283
Coordinates   3819734..3820183 (+) Length   149 a.a.
NCBI ID   WP_014475770.1    Uniprot ID   A0A0D1KPR3
Organism   Bacillus subtilis strain DKU_09     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3814734..3825183
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U7118_RS20505 (U7118_20505) hxlR 3815948..3816310 (-) 363 WP_003246265.1 transcriptional activator HxlR -
  U7118_RS20510 (U7118_20510) hxlA 3816539..3817171 (+) 633 WP_003234604.1 3-hexulose-6-phosphate synthase -
  U7118_RS20515 (U7118_20515) hxlB 3817177..3817734 (+) 558 WP_014478803.1 6-phospho-3-hexuloisomerase -
  U7118_RS20520 (U7118_20520) tlpC 3817845..3819566 (+) 1722 Protein_4037 methyl-accepting chemotaxis protein TlpC -
  U7118_RS20525 (U7118_20525) nucA/comI 3819734..3820183 (+) 450 WP_014475770.1 DNA-entry nuclease Machinery gene
  U7118_RS20530 (U7118_20530) nin/comJ 3820210..3820608 (+) 399 WP_014478800.1 DNA-entry nuclease inhibitor Regulator
  U7118_RS20535 (U7118_20535) bglC 3820645..3822078 (-) 1434 WP_014478799.1 6-phospho-beta-glucosidase -
  U7118_RS20540 (U7118_20540) - 3822151..3822895 (-) 745 Protein_4041 AAA family ATPase -
  U7118_RS20545 (U7118_20545) - 3822914..3823234 (-) 321 WP_014478796.1 DUF3147 family protein -
  U7118_RS20550 (U7118_20550) - 3823222..3823644 (-) 423 WP_014478795.1 MarR family winged helix-turn-helix transcriptional regulator -
  U7118_RS20555 (U7118_20555) yckD 3823772..3824104 (-) 333 WP_014478794.1 YckD family protein -
  U7118_RS20560 (U7118_20560) yckC 3824186..3824641 (-) 456 WP_014478793.1 RDD family protein -
  U7118_RS20565 (U7118_20565) - 3824661..3824858 (-) 198 WP_121509395.1 hypothetical protein -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 16440.39 Da        Isoelectric Point: 4.5282

>NTDB_id=916857 U7118_RS20525 WP_014475770.1 3819734..3820183(+) (nucA/comI) [Bacillus subtilis strain DKU_09]
MNITTDIIKTILLVIVIIAAAAVGLIKGDFFSADQKTSQTKEYDETIAFPSDRYPETAKHIKDAINEGHSEVCTIDRDGA
EERREQSLKDVPSKKGYDRDEWPMAMCKEGGEGASVEYISPADNRGAGSWVGHQLTDYPDGTKVLFTIQ

Nucleotide


Download         Length: 450 bp        

>NTDB_id=916857 U7118_RS20525 WP_014475770.1 3819734..3820183(+) (nucA/comI) [Bacillus subtilis strain DKU_09]
GTGAACATCACGACGGACATCATAAAAACGATACTTCTCGTCATCGTCATCATAGCAGCTGCAGCTGTCGGCCTGATAAA
AGGAGACTTTTTCTCAGCTGATCAAAAAACGTCTCAAACGAAAGAATATGATGAAACAATCGCCTTCCCATCTGACCGGT
ATCCCGAAACTGCCAAGCATATTAAGGATGCGATAAATGAGGGGCACTCAGAGGTGTGCACTATTGACAGAGACGGAGCT
GAAGAACGCCGCGAGCAATCATTAAAGGACGTACCTTCCAAAAAGGGGTATGACAGAGATGAATGGCCAATGGCCATGTG
CAAAGAAGGCGGTGAGGGAGCTTCTGTAGAATACATTTCTCCCGCTGACAACCGCGGAGCAGGCTCTTGGGTCGGGCATC
AGCTTACCGATTACCCAGACGGCACAAAGGTTTTATTCACAATTCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(52-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0D1KPR3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

98.658

100

0.987