Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   U7118_RS09140 Genome accession   NZ_CP141283
Coordinates   1700526..1700936 (+) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   A0A6I4D881
Organism   Bacillus subtilis strain DKU_09     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1695526..1705936
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U7118_RS09115 (U7118_09115) - 1696631..1697521 (-) 891 WP_014480326.1 LysR family transcriptional regulator -
  U7118_RS09120 (U7118_09120) fumC 1697688..1699076 (+) 1389 WP_103330414.1 class II fumarate hydratase -
  U7118_RS09125 (U7118_09125) - 1699295..1699507 (+) 213 Protein_1793 recombinase family protein -
  U7118_RS09130 (U7118_09130) - 1699504..1699602 (-) 99 WP_031600702.1 hypothetical protein -
  U7118_RS09135 (U7118_09135) sigK 1699602..1700330 (-) 729 WP_013308023.1 RNA polymerase sporulation sigma factor SigK -
  U7118_RS09140 (U7118_09140) nucA/comI 1700526..1700936 (+) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  U7118_RS09145 (U7118_09145) yqeB 1700969..1701691 (-) 723 WP_014480321.1 hypothetical protein -
  U7118_RS09150 (U7118_09150) gnd 1701932..1702825 (+) 894 WP_324271581.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  U7118_RS09155 (U7118_09155) yqeD 1702844..1703470 (-) 627 WP_014480319.1 TVP38/TMEM64 family protein -
  U7118_RS09160 (U7118_09160) cwlH 1703657..1704409 (+) 753 WP_017696287.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  U7118_RS09165 (U7118_09165) yqeF 1704661..1705392 (+) 732 WP_029317882.1 SGNH/GDSL hydrolase family protein -
  U7118_RS09170 (U7118_09170) - 1705698..1705838 (-) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=916812 U7118_RS09140 WP_009967785.1 1700526..1700936(+) (nucA/comI) [Bacillus subtilis strain DKU_09]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=916812 U7118_RS09140 WP_009967785.1 1700526..1700936(+) (nucA/comI) [Bacillus subtilis strain DKU_09]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGAGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGCGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGCACCAGAGTGCTGTTTA
TTGTGCAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6I4D881

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529