Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   U6958_RS33250 Genome accession   NZ_CP141274
Coordinates   6951934..6953550 (-) Length   538 a.a.
NCBI ID   WP_016454603.1    Uniprot ID   A0AAX3SLT0
Organism   Delftia lacustris strain DSM 21246     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6946934..6958550
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U6958_RS33235 - 6947531..6948376 (-) 846 WP_026062793.1 hypothetical protein -
  U6958_RS33240 - 6948450..6949589 (-) 1140 WP_074924244.1 slipin family protein -
  U6958_RS33245 rtcR 6950287..6951912 (+) 1626 WP_074924246.1 RNA repair transcriptional activator RtcR -
  U6958_RS33250 rpoN/rpoN1 6951934..6953550 (-) 1617 WP_016454603.1 RNA polymerase factor sigma-54 Machinery gene
  U6958_RS33255 lptB 6953605..6954402 (-) 798 WP_016450697.1 LPS export ABC transporter ATP-binding protein -
  U6958_RS33260 lptA 6954399..6955061 (-) 663 WP_016454602.1 lipopolysaccharide transport periplasmic protein LptA -
  U6958_RS33265 - 6955234..6955884 (-) 651 WP_016450695.1 thiol:disulfide interchange protein DsbA/DsbL -
  U6958_RS33270 - 6955966..6956655 (-) 690 WP_043790827.1 SPOR domain-containing protein -
  U6958_RS33275 argS 6956676..6958373 (-) 1698 WP_016450693.1 arginine--tRNA ligase -

Sequence

Protein


Download         Length: 538 a.a.        Molecular weight: 59267.33 Da        Isoelectric Point: 4.5784

>NTDB_id=916758 U6958_RS33250 WP_016454603.1 6951934..6953550(-) (rpoN/rpoN1) [Delftia lacustris strain DSM 21246]
MKPGLSLRVSQHLSLTPQLQQSIQLLQLSTLELAQEVEQMLGDNPFLELANDDGDHEAASSAPEPAAPGADAADSFQESR
ADQPSAAVEWEAPSDTSSGDAVETSGTAESLSWEGDGQTNSGNDDRDWGSDAPSSRTRGQGDDDETDATELARSAESLTD
HLHRQALSLRLGEVDRAALRFLIENLNDDGYLEDPLDELAQGLAGDDLEECEELVHRFTVALRLLQSLEPVGVGARDLAE
CLTLQLRDQLADAEREGDGSAEDEDRQAVLRTALDICAQPMDLLARRDLRKLASLCGAREERLREAMVLIARLEPRPGRR
FADVQRQIVVPDVIVRPVRMGAGRMEFSVQLNPDVMPKLRVHDIYASALRSHRGSEGHAALQQRLQEARWFIKNIQQRFD
TILRVSQAIVERQKNFFVHGELAMRPLVLRDIADALGLHESTISRVTTAKYMATPQGTYELKYFFGSGLGTEAGGNASST
AVRALIKQFVAAEDPRKPLSDNQLADMLKEQGIECARRTVAKYRELLKIPPTTLRKAL

Nucleotide


Download         Length: 1617 bp        

>NTDB_id=916758 U6958_RS33250 WP_016454603.1 6951934..6953550(-) (rpoN/rpoN1) [Delftia lacustris strain DSM 21246]
TTGAAGCCCGGACTGTCGCTGCGCGTCTCCCAGCATCTTTCGCTCACCCCGCAACTGCAGCAGTCGATACAGCTGCTGCA
GCTGTCCACGCTGGAGCTGGCGCAGGAAGTGGAGCAGATGCTGGGCGACAACCCCTTCCTGGAGCTGGCGAACGACGACG
GCGACCACGAAGCGGCAAGCAGCGCCCCGGAGCCTGCGGCGCCCGGCGCCGACGCCGCGGACTCCTTCCAGGAAAGCCGG
GCGGACCAGCCATCGGCTGCGGTGGAATGGGAGGCGCCGTCGGACACATCCTCCGGCGATGCCGTCGAGACCTCCGGCAC
GGCCGAGTCCCTGAGCTGGGAAGGCGATGGCCAGACCAACTCGGGCAACGACGACCGCGACTGGGGCAGCGATGCGCCTT
CCAGCCGCACGCGCGGCCAGGGTGACGATGACGAGACCGATGCCACCGAACTGGCACGCAGCGCAGAAAGCCTGACCGAC
CATCTGCACCGCCAGGCCCTGAGCCTGCGCCTGGGCGAGGTGGATCGTGCCGCGCTGCGCTTTCTGATCGAGAACCTCAA
CGACGACGGCTACCTGGAAGACCCGCTCGACGAGCTGGCACAGGGCTTGGCGGGCGACGACCTGGAGGAGTGCGAGGAAC
TGGTGCACCGCTTCACCGTGGCGCTGCGCCTGTTGCAAAGCCTGGAGCCGGTGGGCGTGGGCGCGCGCGACCTGGCCGAA
TGCCTGACGCTGCAGCTGCGCGACCAACTGGCCGATGCCGAGCGCGAAGGCGACGGCAGTGCCGAGGACGAAGATCGGCA
GGCCGTGCTGCGCACCGCGCTGGACATCTGCGCCCAGCCCATGGACCTGCTGGCGCGGCGTGACCTGCGCAAGCTCGCCA
GCCTGTGCGGCGCGCGCGAGGAGCGGCTGCGCGAGGCGATGGTCCTGATCGCCCGGCTGGAGCCGCGCCCGGGCCGGCGC
TTCGCCGACGTGCAGCGCCAGATCGTCGTGCCCGATGTCATCGTGCGCCCGGTGCGCATGGGTGCGGGACGCATGGAGTT
CAGCGTGCAACTCAACCCCGATGTCATGCCCAAGCTGCGCGTGCACGACATCTATGCCAGCGCGCTGCGCAGCCACCGGG
GCAGCGAAGGCCATGCGGCGTTGCAGCAGCGGCTGCAGGAGGCGCGCTGGTTCATCAAGAACATCCAGCAGCGCTTCGAC
ACCATCCTGCGCGTGTCGCAGGCCATCGTGGAGCGCCAGAAGAACTTCTTCGTGCATGGCGAGCTGGCCATGCGCCCGCT
GGTGCTGCGCGACATTGCCGATGCGCTGGGCCTGCATGAATCCACCATCAGCCGCGTGACCACGGCCAAGTACATGGCCA
CGCCGCAGGGCACCTACGAACTCAAGTATTTCTTCGGCTCGGGCCTGGGCACCGAGGCGGGCGGCAACGCGTCGAGCACG
GCCGTGCGCGCGCTCATCAAGCAGTTCGTCGCAGCCGAGGACCCCAGGAAGCCGCTGTCGGACAACCAGCTCGCCGACAT
GCTCAAGGAGCAGGGCATCGAATGCGCGCGGCGCACGGTGGCCAAGTACCGCGAGCTGCTCAAGATCCCGCCGACCACGC
TGCGCAAGGCGCTGTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-49)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(149-365)

Sigma-54, DNA binding domain (Sigma54_DBD)

(380-536)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

51.111

100

0.513