Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ABWQ10_RS07715 Genome accession   NZ_CP159917
Coordinates   1309522..1310955 (+) Length   477 a.a.
NCBI ID   WP_000809051.1    Uniprot ID   A0A0H2VDV8
Organism   Escherichia coli strain EC554     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1304522..1315955
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABWQ10_RS07685 (ABWQ10_07685) yrbG 1305080..1306057 (+) 978 WP_000922901.1 calcium/sodium antiporter -
  ABWQ10_RS07690 (ABWQ10_07690) kdsD 1306071..1307057 (+) 987 WP_001295557.1 arabinose-5-phosphate isomerase KdsD -
  ABWQ10_RS07695 (ABWQ10_07695) kdsC 1307078..1307644 (+) 567 WP_000030016.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  ABWQ10_RS07700 (ABWQ10_07700) lptC 1307641..1308216 (+) 576 WP_000030537.1 LPS export ABC transporter periplasmic protein LptC -
  ABWQ10_RS07705 (ABWQ10_07705) lptA 1308185..1308742 (+) 558 WP_000669785.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  ABWQ10_RS07710 (ABWQ10_07710) lptB 1308749..1309474 (+) 726 WP_000224099.1 LPS export ABC transporter ATP-binding protein -
  ABWQ10_RS07715 (ABWQ10_07715) rpoN/rpoN1 1309522..1310955 (+) 1434 WP_000809051.1 RNA polymerase factor sigma-54 Machinery gene
  ABWQ10_RS07720 (ABWQ10_07720) hpf 1310978..1311265 (+) 288 WP_001176599.1 ribosome hibernation promoting factor -
  ABWQ10_RS07725 (ABWQ10_07725) ptsN 1311383..1311874 (+) 492 WP_000183676.1 PTS IIA-like nitrogen regulatory protein PtsN -
  ABWQ10_RS07730 (ABWQ10_07730) rapZ 1311920..1312774 (+) 855 WP_000243741.1 RNase adapter RapZ -
  ABWQ10_RS07735 (ABWQ10_07735) npr 1312771..1313043 (+) 273 WP_000216791.1 PTS phosphocarrier protein NPr -
  ABWQ10_RS07740 (ABWQ10_07740) yrbL 1313257..1313889 (+) 633 WP_000620405.1 PhoP regulatory network protein YrbL -
  ABWQ10_RS07745 (ABWQ10_07745) mtgA 1313886..1314614 (-) 729 WP_000047091.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  ABWQ10_RS07750 (ABWQ10_07750) elbB 1314611..1315265 (-) 655 Protein_1210 isoprenoid biosynthesis glyoxalase ElbB -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 53993.79 Da        Isoelectric Point: 4.3639

>NTDB_id=916085 ABWQ10_RS07715 WP_000809051.1 1309522..1310955(+) (rpoN/rpoN1) [Escherichia coli strain EC554]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESNPLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPE
ELPLDASWDTIYTAGTPSGTSGDYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARLIISDHLDLLANHDFRTLMRVTR
LKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDVLVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIR
SNLQDAKWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=916085 ABWQ10_RS07715 WP_000809051.1 1309522..1310955(+) (rpoN/rpoN1) [Escherichia coli strain EC554]
ATGAAGCAAGGTTTGCAACTCAGGCTTAGCCAACAACTGGCGATGACGCCACAGCTCCAACAGGCAATTCGTCTGTTGCA
GTTGTCGACGCTGGAACTTCAGCAGGAGCTACAGCAGGCGCTGGAGAGTAATCCGCTGCTTGAGCAAATCGACACTCATG
AAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAA
GAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGA
TGAGCTGCCGGTCTATCAGGGCGAAACGACGCAGACCTTGCAGGATTACCTGATGTGGCAGGTCGAGCTGACACCGTTTT
CCGACACTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGACACCGGTTATCTGACTGTCCCGCTGGAAGAT
ATTCTCGAAAGTATGGGCGATGAAGAGATCGACATCGACGAGGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCC
GGTCGGTGTAGCGGCAAAAGATCTGCGTGACTGCCTGCTAATCCAACTCTCCCAATTCGATAAAACCACGCCGTGGCTGG
AAGAGGCCAGACTGATCATTAGCGATCATCTCGATCTGTTAGCCAATCACGACTTCCGCACTTTAATGCGCGTCACGCGT
CTGAAAGAAGATGTGCTGAAAGAAGCCGTCAATCTGATCCAGTCGCTCGATCCGCGCCCCGGGCAGTCGATCCAGACTGG
CGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAACTCAACAGTGACAGCA
TTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGC
AGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGTCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTAT
CGTTGAACAGCAGCAAGCCTTCTTTGAGCAAGGTGAAGAATATATGAAACCGATGGTACTGGCCGATATCGCCCAGGCTG
TCGAAATGCATGAATCGACGATATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAG
TATTTCTTTTCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCACTGGTGAAGAAATT
AATCGCGGCGGAAAACCCAGCGAAACCGTTGAGCGACAGCAAGTTAACCTCTTTACTGTCGGAACAAGGTATCATGGTGG
CACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAGCTCGTTTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2VDV8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

44.223

100

0.465