Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ABYD36_RS26710 Genome accession   NZ_CP159875
Coordinates   5143897..5145330 (+) Length   477 a.a.
NCBI ID   WP_032276664.1    Uniprot ID   -
Organism   Escherichia coli strain 12089     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5138897..5150330
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABYD36_RS26680 (ABYD36_26680) yrbG 5139455..5140432 (+) 978 WP_000922901.1 calcium/sodium antiporter -
  ABYD36_RS26685 (ABYD36_26685) kdsD 5140446..5141432 (+) 987 WP_001295557.1 arabinose-5-phosphate isomerase KdsD -
  ABYD36_RS26690 (ABYD36_26690) kdsC 5141453..5142019 (+) 567 WP_000030018.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  ABYD36_RS26695 (ABYD36_26695) lptC 5142016..5142591 (+) 576 WP_000030537.1 LPS export ABC transporter periplasmic protein LptC -
  ABYD36_RS26700 (ABYD36_26700) lptA 5142560..5143117 (+) 558 WP_000669785.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  ABYD36_RS26705 (ABYD36_26705) lptB 5143124..5143849 (+) 726 WP_000224099.1 LPS export ABC transporter ATP-binding protein -
  ABYD36_RS26710 (ABYD36_26710) rpoN/rpoN1 5143897..5145330 (+) 1434 WP_032276664.1 RNA polymerase factor sigma-54 Machinery gene
  ABYD36_RS26715 (ABYD36_26715) hpf 5145353..5145640 (+) 288 WP_001176599.1 ribosome hibernation promoting factor -
  ABYD36_RS26720 (ABYD36_26720) ptsN 5145758..5146249 (+) 492 WP_000183676.1 PTS IIA-like nitrogen regulatory protein PtsN -
  ABYD36_RS26725 (ABYD36_26725) rapZ 5146295..5147149 (+) 855 WP_000243741.1 RNase adapter RapZ -
  ABYD36_RS26730 (ABYD36_26730) npr 5147146..5147418 (+) 273 WP_000216791.1 PTS phosphocarrier protein NPr -
  ABYD36_RS26735 (ABYD36_26735) yrbL 5147632..5148264 (+) 633 WP_000620405.1 PhoP regulatory network protein YrbL -
  ABYD36_RS26740 (ABYD36_26740) mtgA 5148261..5148989 (-) 729 WP_000047091.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  ABYD36_RS26745 (ABYD36_26745) elbB 5148986..5149639 (-) 654 WP_001299134.1 isoprenoid biosynthesis glyoxalase ElbB -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54020.82 Da        Isoelectric Point: 4.3639

>NTDB_id=915406 ABYD36_RS26710 WP_032276664.1 5143897..5145330(+) (rpoN/rpoN1) [Escherichia coli strain 12089]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESNPLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPE
ELPLDASWDTIYTAGTPSGTSGDYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARLIINDHLDLLANHDFRTLMRVTR
LKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDVLVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIR
SNLQDAKWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=915406 ABYD36_RS26710 WP_032276664.1 5143897..5145330(+) (rpoN/rpoN1) [Escherichia coli strain 12089]
ATGAAGCAAGGTTTGCAACTCAGGCTTAGCCAACAACTGGCGATGACGCCACAGCTCCAACAGGCAATTCGTCTGTTGCA
GTTGTCGACGCTGGAACTTCAGCAGGAGCTACAGCAGGCGCTGGAGAGTAATCCGCTGCTTGAGCAAATCGACACTCATG
AAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACGCTGGACACCGCCGATGCGCTCGAACAAAAAGAGATGCCGGAA
GAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGA
TGAGCTGCCGGTCTATCAGGGCGAAACGACGCAGACCTTGCAGGATTACCTGATGTGGCAGGTCGAGCTGACACCGTTTT
CCGACACTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGACACCGGTTATCTGACTGTCCCGCTGGAAGAT
ATTCTCGAAAGTATGGGCGATGAAGAGATCGACATCGACGAGGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCC
GGTCGGTGTAGCGGCAAAAGATCTGCGTGACTGCCTGCTAATCCAACTCTCCCAATTCGATAAAACCACGCCGTGGCTGG
AAGAGGCCAGATTGATCATTAACGATCATCTCGATCTGTTAGCCAATCACGACTTCCGCACTTTAATGCGCGTCACGCGT
CTGAAAGAAGATGTGCTGAAAGAAGCCGTCAATCTGATCCAGTCGCTCGATCCGCGCCCCGGGCAGTCGATCCAGACTGG
CGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACAGTAGAACTCAACAGTGACAGCA
TTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCTCGCAATGATGGTGACAGCCAGTTTATCCGC
AGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGTCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTAT
CGTTGAACAGCAGCAAGCCTTCTTTGAGCAAGGCGAAGAATATATGAAACCGATGGTACTGGCCGATATCGCCCAGGCTG
TCGAAATGCATGAATCGACGATATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAG
TATTTCTTTTCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCGCTGGTGAAGAAATT
AATCGCGGCGGAAAACCCAGCGAAACCGTTGAGCGACAGCAAGTTAACCTCTTTGCTGTCGGAACAAGGTATCATGGTGG
CACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAGCTCGTTTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

44.422

100

0.468