Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ABXJ32_RS19645 Genome accession   NZ_CP159728
Coordinates   4049625..4051058 (-) Length   477 a.a.
NCBI ID   WP_000809051.1    Uniprot ID   A0A0H2VDV8
Organism   Escherichia coli strain Z22-6     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4044625..4056058
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABXJ32_RS19610 (ABXJ32_19605) elbB 4045316..4045969 (+) 654 WP_001299134.1 isoprenoid biosynthesis glyoxalase ElbB -
  ABXJ32_RS19615 (ABXJ32_19610) mtgA 4045966..4046694 (+) 729 WP_000047091.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  ABXJ32_RS19620 (ABXJ32_19615) yrbL 4046691..4047323 (-) 633 WP_000620405.1 PhoP regulatory network protein YrbL -
  ABXJ32_RS19625 (ABXJ32_19620) npr 4047537..4047809 (-) 273 WP_097449392.1 PTS phosphocarrier protein NPr -
  ABXJ32_RS19630 (ABXJ32_19625) rapZ 4047806..4048660 (-) 855 WP_000243741.1 RNase adapter RapZ -
  ABXJ32_RS19635 (ABXJ32_19630) ptsN 4048706..4049197 (-) 492 WP_000183676.1 PTS IIA-like nitrogen regulatory protein PtsN -
  ABXJ32_RS19640 (ABXJ32_19635) hpf 4049315..4049602 (-) 288 WP_001176599.1 ribosome hibernation promoting factor -
  ABXJ32_RS19645 (ABXJ32_19640) rpoN/rpoN1 4049625..4051058 (-) 1434 WP_000809051.1 RNA polymerase factor sigma-54 Machinery gene
  ABXJ32_RS19650 (ABXJ32_19645) lptB 4051106..4051831 (-) 726 WP_000224099.1 LPS export ABC transporter ATP-binding protein -
  ABXJ32_RS19655 (ABXJ32_19650) lptA 4051838..4052395 (-) 558 WP_000669785.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  ABXJ32_RS19660 (ABXJ32_19655) lptC 4052364..4052939 (-) 576 WP_000030537.1 LPS export ABC transporter periplasmic protein LptC -
  ABXJ32_RS19665 (ABXJ32_19660) kdsC 4052936..4053502 (-) 567 WP_000030016.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  ABXJ32_RS19670 (ABXJ32_19665) kdsD 4053523..4054509 (-) 987 WP_001295557.1 arabinose-5-phosphate isomerase KdsD -
  ABXJ32_RS19675 (ABXJ32_19670) yrbG 4054523..4055500 (-) 978 WP_000922901.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 53993.79 Da        Isoelectric Point: 4.3639

>NTDB_id=914641 ABXJ32_RS19645 WP_000809051.1 4049625..4051058(-) (rpoN/rpoN1) [Escherichia coli strain Z22-6]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESNPLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPE
ELPLDASWDTIYTAGTPSGTSGDYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARLIISDHLDLLANHDFRTLMRVTR
LKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDVLVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIR
SNLQDAKWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=914641 ABXJ32_RS19645 WP_000809051.1 4049625..4051058(-) (rpoN/rpoN1) [Escherichia coli strain Z22-6]
ATGAAGCAAGGTTTGCAACTCAGGCTTAGCCAACAACTGGCGATGACGCCACAGCTCCAACAGGCAATTCGTCTGTTGCA
GTTGTCGACGCTGGAACTTCAGCAGGAGCTACAGCAGGCGCTGGAGAGTAATCCGCTGCTTGAGCAAATCGACACTCATG
AAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAA
GAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGA
TGAGCTGCCGGTCTATCAGGGCGAAACGACGCAGACCTTGCAGGATTACCTGATGTGGCAGGTCGAGCTGACACCGTTTT
CCGACACTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGACACCGGTTATCTGACTGTCCCGCTGGAAGAT
ATTCTCGAAAGTATGGGCGATGAAGAGATCGACATCGACGAGGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCC
GGTCGGTGTAGCGGCAAAAGATCTGCGTGACTGCCTGCTAATCCAACTCTCCCAATTCGATAAAACCACGCCGTGGCTGG
AAGAGGCCAGACTGATCATTAGCGATCATCTCGATCTGTTAGCCAATCACGACTTCCGCACTTTAATGCGCGTCACGCGT
CTGAAAGAAGATGTGCTGAAAGAAGCCGTCAATCTGATCCAGTCGCTCGATCCGCGCCCCGGGCAGTCGATCCAGACTGG
CGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAACTCAACAGTGACAGCA
TTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGC
AGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGTCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTAT
CGTTGAACAGCAGCAAGCCTTCTTTGAGCAAGGTGAAGAATATATGAAACCGATGGTACTGGCCGATATCGCCCAGGCTG
TCGAAATGCATGAATCGACGATATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAG
TATTTCTTTTCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCGCTGGTGAAGAAATT
AATCGCGGCGGAAAACCCAGCGAAACCGTTGAGCGACAGCAAGTTAACCTCTTTACTGTCGGAACAAGGTATCATGGTGG
CACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAGCTCGTTTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2VDV8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

44.223

100

0.465