Detailed information    

insolico Bioinformatically predicted

Overview

Name   uvrC   Type   Machinery gene
Locus tag   SPH25_RS02645 Genome accession   NZ_CP140484
Coordinates   541278..543062 (-) Length   594 a.a.
NCBI ID   WP_000774350.1    Uniprot ID   -
Organism   Helicobacter pylori strain 52N     
Function   integration of short DNA fragment (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 536278..548062
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SPH25_RS02615 - 536823..537071 (+) 249 WP_000790557.1 RNA-binding protein -
  SPH25_RS02620 - 537277..538098 (-) 822 WP_014533429.1 glycosyltransferase family 25 protein -
  SPH25_RS02625 trxB 538306..539241 (-) 936 WP_000564454.1 thioredoxin-disulfide reductase -
  SPH25_RS02630 trxA 539247..539567 (-) 321 WP_000020200.1 thioredoxin -
  SPH25_RS02635 - 539657..540001 (-) 345 WP_001211660.1 YraN family protein -
  SPH25_RS02640 - 540002..541267 (-) 1266 WP_000746728.1 homoserine dehydrogenase -
  SPH25_RS02645 uvrC 541278..543062 (-) 1785 WP_000774350.1 excinuclease ABC subunit UvrC Machinery gene
  SPH25_RS02650 - 543063..543524 (-) 462 WP_000437194.1 hypothetical protein -
  SPH25_RS02655 - 543516..543695 (+) 180 WP_000468796.1 hypothetical protein -
  SPH25_RS02660 - 543707..544348 (-) 642 WP_000659453.1 ATP-binding cassette domain-containing protein -
  SPH25_RS02665 - 544350..546011 (-) 1662 WP_001139656.1 ABC transporter permease/substrate-binding protein -
  SPH25_RS02670 - 546128..547552 (+) 1425 WP_000855793.1 DNA methyltransferase -

Sequence

Protein


Download         Length: 594 a.a.        Molecular weight: 68743.97 Da        Isoelectric Point: 9.6675

>NTDB_id=914463 SPH25_RS02645 WP_000774350.1 541278..543062(-) (uvrC) [Helicobacter pylori strain 52N]
MADLLSSLKNLSHSSGVYQYFDKNRQLLYIGKAKNLKKRIKSYFSIHNNEITPNHRASLRIQMMVKQIAFLETILVENEQ
DALILENSLIKQLKPKYNILLRDDKTYPYIYMDFSTDFPIPLITRKILKQPGVKYFGPFTSGAKDILDSLYELLPLVQKK
NCIKDKKACMFYQIERCKAPCENKITKEEYLKIAKECLEMIENKDKLIKELELKMERLSNNLRFEEALIYRDRIAKIQKI
APFTCMDLAKLYDLDIFAFYSGSNKAVLVKMFMRGGKIISSAFEKIHSLNGFDTDEAMKQAIINHYQSHLPLMPEQILLN
ACSNETLKELQEFISHQYSKKIALSIPKKGDKLALIEIAMKNAQEIFSQEKTSNEDLILEEARSLFKLECMPYRVEIFDV
SHHANSQCVGGMVVYENHEFQKNSYRRYHLKGSNEYTQMSELLTRRALDFAKEPPPNLWVIDGGRAQLNIALEILKSSGS
FVEVIAISKEKRDSKAYRSKGGAKDIIHTASDTFKLLPSDKRLQWVQKLRDESHRYAINFHRSTKLKNMKQIALLKEKGI
GEASVKKLLDYFGSFEAIEKASEQEKNAVLKKRN

Nucleotide


Download         Length: 1785 bp        

>NTDB_id=914463 SPH25_RS02645 WP_000774350.1 541278..543062(-) (uvrC) [Helicobacter pylori strain 52N]
ATGGCTGATTTATTGTCCAGTTTGAAAAACCTTTCTCATAGCAGTGGCGTGTATCAATATTTTGATAAAAACCGCCAATT
ACTCTATATCGGTAAGGCGAAAAATTTAAAAAAGCGCATCAAAAGTTATTTTTCTATCCACAATAATGAAATCACGCCCA
ATCATCGCGCAAGCTTACGAATCCAAATGATGGTCAAACAGATCGCTTTTTTAGAAACCATTTTAGTGGAAAACGAGCAA
GACGCTTTGATCTTAGAAAATTCTTTAATCAAACAGCTCAAGCCCAAATACAACATTCTTTTAAGAGATGATAAAACTTA
CCCTTATATTTATATGGATTTTTCCACCGATTTCCCTATCCCTTTAATCACACGAAAAATTTTAAAACAGCCTGGCGTTA
AATATTTTGGCCCTTTTACGAGCGGGGCTAAGGATATTTTAGACAGCTTGTATGAATTGCTCCCGTTAGTTCAAAAGAAA
AATTGCATCAAAGATAAAAAGGCATGCATGTTTTATCAAATAGAGCGTTGTAAAGCCCCATGCGAGAATAAAATCACCAA
AGAAGAGTATTTGAAAATCGCTAAAGAATGTTTAGAAATGATTGAAAATAAAGACAAACTCATCAAAGAGCTTGAATTAA
AAATGGAGCGCCTTTCTAATAACTTGCGTTTTGAAGAAGCCCTCATTTATAGGGACAGGATTGCAAAAATCCAAAAAATC
GCCCCTTTCACTTGCATGGATTTAGCCAAACTCTATGATTTGGATATTTTTGCTTTTTATAGTGGGAGCAATAAGGCGGT
GTTGGTGAAAATGTTCATGCGTGGGGGTAAAATCATTTCTTCAGCGTTTGAAAAAATCCACTCTCTCAATGGGTTTGACA
CTGATGAAGCGATGAAACAAGCCATTATCAATCATTACCAATCGCATTTGCCTTTGATGCCTGAACAGATTTTATTGAAC
GCTTGCTCTAATGAAACGCTTAAAGAATTGCAAGAATTTATCTCTCACCAATACTCTAAAAAAATCGCTCTTAGCATTCC
TAAAAAAGGCGATAAGCTCGCTTTAATAGAAATCGCTATGAAAAACGCTCAAGAGATTTTTAGCCAAGAAAAAACCTCTA
ATGAAGATCTGATTTTAGAAGAAGCGCGATCGCTCTTTAAATTAGAGTGCATGCCTTATAGGGTGGAAATCTTTGATGTA
AGCCACCATGCCAACAGCCAATGCGTGGGGGGAATGGTCGTGTATGAAAATCATGAATTTCAAAAAAACTCCTATCGGCG
CTACCATTTAAAAGGCTCTAATGAATACACTCAAATGAGCGAATTGCTCACCAGAAGGGCTTTAGATTTTGCCAAAGAGC
CACCGCCTAATTTGTGGGTGATCGATGGAGGGAGGGCGCAATTAAACATCGCTTTAGAAATTTTAAAAAGCAGTGGGAGT
TTTGTAGAAGTGATCGCTATTTCTAAAGAAAAAAGGGATTCTAAAGCTTATCGCTCTAAAGGGGGCGCTAAAGACATTAT
CCACACCGCTAGCGATACTTTTAAATTGCTCCCTAGCGACAAACGCTTGCAATGGGTGCAAAAATTGCGCGATGAAAGCC
ACCGGTATGCGATAAACTTCCATAGATCCACTAAACTTAAAAACATGAAACAAATCGCTCTTTTAAAAGAAAAGGGTATA
GGAGAAGCCAGCGTGAAAAAATTGTTGGATTATTTTGGGAGTTTTGAAGCGATAGAAAAAGCGAGCGAGCAGGAAAAAAA
CGCCGTTTTAAAAAAACGAAATTAA

Domains

Predicted by InterproScan.

GIY-YIG catalytic domain (GIY-YIG)

(15-98)

UvrB/uvrC motif (UVR)

(205-239)

UvrC RNAse H endonuclease domain (UvrC_RNaseH_dom)

(385-538)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  uvrC Helicobacter pylori 26695

97.639

99.832

0.975