Detailed information    

experimental Experimentally validated

Overview

Name   uvrC   Type   Machinery gene
Locus tag   C694_RS04180 Genome accession   NC_018939
Coordinates   873406..875190 (+) Length   594 a.a.
NCBI ID   WP_000774319.1    Uniprot ID   A0AAC8SBE8
Organism   Helicobacter pylori 26695     
Function   integration of short DNA fragment   
DNA binding and uptake

Function

UvrC, an endonuclease involved in DNA repair, is responsible for the specific integration of short DNA fragments. This suggests that short and long fragments are imported through distinct recombination pathways.


Genomic Context

Location: 868406..880190
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C694_RS04150 (C694_04180) motB 869170..869943 (+) 774 WP_015056062.1 flagellar motor protein MotB -
  C694_RS04155 (C694_04185) - 869940..870386 (+) 447 WP_001081241.1 hypothetical protein -
  C694_RS04160 (C694_04190) - 870455..872116 (+) 1662 WP_000901317.1 glycine betaine ABC transporter substrate-binding protein -
  C694_RS04165 (C694_04195) - 872120..872770 (+) 651 WP_000659451.1 ATP-binding cassette domain-containing protein -
  C694_RS04170 - 872773..872952 (-) 180 WP_000468790.1 hypothetical protein -
  C694_RS04175 (C694_04200) - 872944..873405 (+) 462 WP_000437145.1 hypothetical protein -
  C694_RS04180 (C694_04205) uvrC 873406..875190 (+) 1785 WP_000774319.1 excinuclease ABC subunit UvrC Machinery gene
  C694_RS04185 (C694_04210) - 875201..876466 (+) 1266 WP_000746824.1 homoserine dehydrogenase -
  C694_RS04190 (C694_04215) - 876467..876811 (+) 345 WP_001211699.1 YraN family protein -
  C694_RS04195 (C694_04220) trxA 876900..877220 (+) 321 WP_000020199.1 thioredoxin -
  C694_RS04200 (C694_04225) trxB 877225..878160 (+) 936 WP_000564420.1 thioredoxin-disulfide reductase -
  C694_RS04205 (C694_04230) - 878376..879197 (+) 822 WP_001262061.1 glycosyltransferase family 25 protein -
  C694_RS04210 (C694_04235) - 879403..879651 (-) 249 WP_001244602.1 RNA-binding protein -
  C694_RS08990 - 879805..879996 (+) 192 Protein_798 hypothetical protein -

Sequence

Protein


Download         Length: 594 a.a.        Molecular weight: 68679.95 Da        Isoelectric Point: 9.7128

>NTDB_id=1206 C694_RS04180 WP_000774319.1 873406..875190(+) (uvrC) [Helicobacter pylori 26695]
MADLLSSLKNLPNSSGVYQYFDKNRQLLYIGKAKNLKKRIKSYFSIRNNEITPNHRASLRIQMMVKQIAFLETILVENEQ
DALILENSLIKQLKPKYNILLRDDKTYPYIYMDFSTDFPIPLITRKILKQPGVKYFGPFTSGAKDILDSLYELLPLVQKK
NCIKDKKACIFYQIERCKAPCENKITKEEYLKIAKECLEMIENKDRLIKELELKMERLSNNLRFEEALIYRDRIAKIQKI
APFTCMDLAKLYDLDIFAFYGASNKAVLVKMFMRGGKIISSAFEKIHSLNGFDTDEAMKQAIINHYQSHLPLMPEQILLN
ACSNETLKELQEFISHQYSKKIALSIPKKGDKLALIEIAMKNAQEIFSQEKTSNEDLILEEARSLFKLECMPYRVEIFDT
SHHSSSQCVGGMVVYENNAFQKNSYRRYHLKGSDEYTQMSELLTRRALDFAKEPPPNLWVIDGGRAQLNIALEILKSSGS
FVEVIAISKEKRDSKAYRSKGGAKDIIHTPSDTFKLLPSDKRLQWVQKLRDESHRYAINFHRSTKLKNMKQIALLKEKGI
GEASVKKLLDYFGSFEAIEKASEQEKNAVLKKRI

Nucleotide


Download         Length: 1785 bp        

>NTDB_id=1206 C694_RS04180 WP_000774319.1 873406..875190(+) (uvrC) [Helicobacter pylori 26695]
ATGGCTGATTTATTGTCCAGTTTAAAAAACCTTCCTAACAGCAGTGGCGTGTATCAATATTTTGATAAAAACCGCCAATT
ACTCTATATCGGTAAGGCGAAAAATTTAAAAAAACGCATCAAAAGCTATTTTTCCATCCGCAATAATGAAATCACGCCCA
ATCATCGCGCCAGCTTACGCATCCAAATGATGGTCAAACAGATCGCTTTTTTAGAAACCATTTTAGTGGAAAACGAGCAA
GACGCTTTGATTTTAGAAAACTCTTTAATCAAGCAGCTCAAACCCAAATACAACATTCTTTTAAGAGACGATAAAACTTA
CCCTTATATTTATATGGATTTTTCCACTGATTTCCCTATCCCTTTAATCACACGAAAAATTTTAAAACAGCCTGGCGTTA
AATATTTTGGCCCTTTTACGAGCGGGGCTAAGGATATTTTGGACAGCTTGTATGAATTGCTCCCGTTAGTTCAAAAGAAA
AATTGCATCAAGGATAAAAAGGCATGCATTTTTTATCAAATAGAGCGTTGTAAAGCCCCATGCGAGAATAAAATCACCAA
AGAAGAGTATTTAAAAATCGCTAAAGAATGTTTAGAAATGATTGAAAATAAAGACAGGCTCATCAAGGAGCTTGAATTAA
AAATGGAGCGCCTTTCTAATAACTTGCGTTTTGAAGAAGCCCTAATTTACAGGGACAGGATTGCAAAAATCCAAAAAATC
GCCCCTTTCACTTGCATGGATTTAGCCAAACTCTATGATTTGGATATTTTTGCTTTTTATGGCGCAAGCAATAAAGCGGT
GTTAGTGAAAATGTTCATGCGTGGGGGTAAAATCATTTCTTCAGCGTTTGAAAAAATCCACTCTCTTAATGGGTTTGACA
CTGATGAAGCGATGAAACAAGCCATTATCAATCATTACCAATCGCATTTGCCTTTGATGCCAGAACAGATTTTATTGAAC
GCTTGCTCTAATGAAACGCTTAAAGAATTGCAAGAATTTATCTCTCACCAATACTCTAAAAAAATCGCTCTTAGCATTCC
TAAAAAAGGCGACAAGCTCGCTTTAATAGAAATCGCTATGAAAAACGCTCAAGAGATTTTTAGCCAAGAAAAAACCTCTA
ATGAAGATCTGATTTTAGAAGAAGCGCGATCGCTCTTTAAATTAGAGTGCATGCCTTATAGGGTAGAAATCTTTGACACA
AGCCACCATTCTAGCAGCCAATGCGTGGGGGGAATGGTCGTGTATGAAAACAACGCCTTCCAAAAAAACTCTTATCGGCG
CTACCATTTAAAAGGCTCTGATGAATACACTCAAATGAGCGAATTGCTCACCAGAAGGGCTTTAGACTTTGCCAAAGAGC
CACCGCCTAATTTGTGGGTGATCGATGGAGGGAGGGCACAATTAAACATCGCTTTAGAAATTTTAAAAAGCAGCGGGAGT
TTTGTGGAAGTGATCGCTATTTCTAAAGAAAAAAGGGATTCTAAAGCTTATCGCTCTAAAGGGGGCGCTAAAGACATTAT
CCATACGCCTAGCGATACTTTTAAATTGCTCCCTAGCGACAAACGCTTGCAGTGGGTGCAAAAATTGCGCGATGAAAGCC
ACCGGTATGCGATAAACTTCCATAGATCCACTAAACTTAAAAACATGAAACAAATCGCTCTTTTAAAAGAAAAGGGTATA
GGAGAAGCCAGCGTGAAAAAATTATTGGATTATTTTGGGAGTTTTGAAGCGATAGAAAAAGCGAGCGAGCAGGAAAAAAA
CGCCGTTTTAAAAAAACGAATCTAA

Domains

Predicted by InterproScan.

UvrC RNAse H endonuclease domain (UvrC_RNaseH_dom)

(385-538)

UvrB/uvrC motif (UVR)

(205-239)

GIY-YIG catalytic domain (GIY-YIG)

(15-98)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] Florent Ailloud et al. (2022) The Helicobacter pylori UvrC Nuclease Is Essential for Chromosomal Microimports after Natural Transformation. MBio 13(4):e0181122. [PMID: 35876509]