Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilV   Type   Machinery gene
Locus tag   U2S93_RS27230 Genome accession   NZ_CP140431
Coordinates   5713398..5713955 (+) Length   185 a.a.
NCBI ID   WP_003094697.1    Uniprot ID   A0A6N0KGD7
Organism   Pseudomonas aeruginosa strain 2023CK-01249     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5708398..5718955
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U2S93_RS27210 (U2S93_27210) pilR 5709674..5711011 (+) 1338 WP_003094694.1 two-component system response regulator PilR Regulator
  U2S93_RS27215 (U2S93_27215) thiO 5711057..5712151 (-) 1095 WP_009316267.1 glycine oxidase ThiO -
  U2S93_RS27220 (U2S93_27220) - 5712286..5712795 (+) 510 WP_015503652.1 GspH/FimT family pseudopilin -
  U2S93_RS27225 (U2S93_27225) - 5712901..5713407 (+) 507 WP_003094696.1 GspH/FimT family pseudopilin -
  U2S93_RS27230 (U2S93_27230) pilV 5713398..5713955 (+) 558 WP_003094697.1 type 4a pilus minor pilin PilV Machinery gene
  U2S93_RS27235 (U2S93_27235) pilW 5713952..5714776 (+) 825 WP_003094698.1 type 4a pilus minor pilin PilW -
  U2S93_RS27240 (U2S93_27240) pilX 5714773..5715360 (+) 588 WP_003094700.1 type 4a pilus minor pilin PilX -
  U2S93_RS27245 (U2S93_27245) pilY1 5715372..5718863 (+) 3492 WP_022580136.1 type 4a pilus biogenesis protein PilY1 -

Sequence

Protein


Download         Length: 185 a.a.        Molecular weight: 20069.21 Da        Isoelectric Point: 8.2164

>NTDB_id=914250 U2S93_RS27230 WP_003094697.1 5713398..5713955(+) (pilV) [Pseudomonas aeruginosa strain 2023CK-01249]
MLLKSRHKPLYQSGFSMIEVLVALLLISIGVLGMIAMQGKTIQYTADSVERNKAAMLGSNLLESMRASPKALYDVKDQMA
TQSDFFKAKGSAFPTAPSSCTPLPDAIKDRLGCWAEQVKNELPGAGDLLKSDYYICRSSKPGDCDGKGSMLEIRLAWRGK
QGACVNAADSSADTSLCYYTLRVEP

Nucleotide


Download         Length: 558 bp        

>NTDB_id=914250 U2S93_RS27230 WP_003094697.1 5713398..5713955(+) (pilV) [Pseudomonas aeruginosa strain 2023CK-01249]
ATGCTATTGAAATCGCGGCACAAGCCGCTGTACCAATCCGGCTTCAGCATGATCGAAGTGCTGGTCGCCCTGCTGCTGAT
CAGCATCGGCGTGCTGGGCATGATCGCCATGCAGGGCAAGACCATCCAGTACACCGCCGACTCGGTCGAGCGCAACAAGG
CAGCCATGCTCGGCAGCAACCTGCTGGAAAGCATGCGGGCGAGCCCGAAGGCGCTGTACGACGTCAAGGACCAGATGGCC
ACGCAATCCGACTTCTTCAAGGCCAAGGGGTCGGCGTTCCCCACCGCGCCGTCCTCCTGCACGCCATTGCCCGACGCGAT
CAAGGACCGCCTGGGATGCTGGGCGGAACAGGTGAAGAACGAACTGCCAGGCGCTGGCGACCTGCTGAAGAGCGACTACT
ACATCTGCCGCAGCTCGAAGCCAGGCGACTGCGACGGCAAGGGCTCCATGCTGGAAATCCGCCTGGCCTGGCGCGGCAAG
CAAGGCGCCTGCGTCAACGCCGCCGACAGCAGCGCCGACACCTCCCTCTGCTACTACACCCTCAGGGTCGAGCCATGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(12-34)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6N0KGD7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilV Pseudomonas aeruginosa PAK

97.838

100

0.978