Detailed information    

insolico Bioinformatically predicted

Overview

Name   ruvB   Type   Machinery gene
Locus tag   AABK14_RS05075 Genome accession   NZ_AP027886
Coordinates   1036448..1037458 (-) Length   336 a.a.
NCBI ID   WP_000568519.1    Uniprot ID   Q32HA1
Organism   Escherichia coli strain 2018-05-5CC     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1031448..1042458
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AABK14_RS05055 (VEE47_09790) mepM 1032221..1033543 (-) 1323 WP_001184045.1 murein DD-endopeptidase MepM -
  AABK14_RS05060 (VEE47_09800) znuA 1033559..1034491 (-) 933 WP_001300644.1 zinc ABC transporter substrate-binding protein ZnuA -
  AABK14_RS05065 (VEE47_09810) znuC 1034570..1035325 (+) 756 WP_095186582.1 zinc ABC transporter ATP-binding protein ZnuC -
  AABK14_RS05070 (VEE47_09820) znuB 1035322..1036107 (+) 786 WP_000571480.1 zinc ABC transporter permease subunit ZnuB -
  AABK14_RS05075 (VEE47_09830) ruvB 1036448..1037458 (-) 1011 WP_000568519.1 Holliday junction branch migration DNA helicase RuvB Machinery gene
  AABK14_RS05080 (VEE47_09840) ruvA 1037467..1038078 (-) 612 WP_000580327.1 Holliday junction branch migration protein RuvA -
  AABK14_RS05085 yobI 1038217..1038282 (-) 66 WP_072094247.1 stress response small protein YobI -
  AABK14_RS05090 (VEE47_09850) yebB 1038353..1038955 (+) 603 WP_094336969.1 YebB family permuted papain-like enzyme -
  AABK14_RS05095 (VEE47_09860) ruvC 1038957..1039478 (-) 522 WP_001295503.1 crossover junction endodeoxyribonuclease RuvC -
  AABK14_RS05100 (VEE47_09870) yebC 1039513..1040253 (-) 741 WP_000907234.1 YebC/PmpR family DNA-binding transcriptional regulator -
  AABK14_RS05105 (VEE47_09880) nudB 1040282..1040734 (-) 453 WP_001300367.1 dihydroneopterin triphosphate diphosphatase -

Sequence

Protein


Download         Length: 336 a.a.        Molecular weight: 37173.77 Da        Isoelectric Point: 4.7818

>NTDB_id=91407 AABK14_RS05075 WP_000568519.1 1036448..1037458(-) (ruvB) [Escherichia coli strain 2018-05-5CC]
MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIFIKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMG
VNLRTTSGPVLEKAGDLAAMLTNLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARGTPRIANRLLRRVRDFAEVKHDG
TISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKFFGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMA
TTRAWNHFGITPPEMP

Nucleotide


Download         Length: 1011 bp        

>NTDB_id=91407 AABK14_RS05075 WP_000568519.1 1036448..1037458(-) (ruvB) [Escherichia coli strain 2018-05-5CC]
ATGATTGAAGCAGACCGTCTGATTTCTGCCGGTACCACTTTGCCGGAAGATGTAGCAGATCGCGCCATTCGCCCTAAATT
ACTGGAAGAGTATGTTGGTCAGCCGCAGGTTCGTTCACAGATGGAGATTTTCATCAAAGCAGCGAAACTGCGCGGCGATG
CCCTCGATCATTTGTTGATTTTTGGTCCTCCGGGGTTGGGTAAAACTACGCTTGCCAACATTGTCGCCAATGAAATGGGC
GTTAATTTACGCACGACTTCTGGTCCGGTGCTGGAAAAGGCGGGCGATTTGGCTGCGATGCTCACTAACCTTGAACCGCA
TGACGTGCTGTTTATTGATGAGATCCACCGTTTATCGCCAGTTGTTGAAGAAGTGCTGTACCCGGCAATGGAAGACTACC
AACTGGATATCATGATTGGTGAAGGTCCGGCGGCACGCTCCATTAAAATTGATTTGCCGCCGTTTACCCTGATTGGTGCA
ACCACGCGCGCAGGTTCGCTGACATCACCGTTGCGCGACCGTTTTGGGATTGTGCAACGTCTGGAGTTTTATCAGGTGCC
GGATCTGCAATATATCGTCAGTCGCAGCGCACGCTTTATGGGGCTTGAGATGAGTGATGACGGGGCGCTGGAAGTTGCTC
GTCGCGCTCGCGGTACGCCGCGCATTGCCAACCGTCTGCTGCGTCGAGTGCGTGATTTCGCCGAAGTGAAGCACGATGGC
ACCATCTCGGCAGATATCGCTGCTCAGGCGTTGGATATGTTGAATGTCGATGCTGAAGGTTTCGATTATATGGACCGCAA
GTTATTGCTGGCGGTAATCGATAAGTTCTTTGGTGGGCCGGTAGGTCTGGATAACCTTGCGGCAGCCATTGGCGAAGAAC
GTGAAACCATTGAGGATGTGCTGGAACCTTATTTGATTCAGCAAGGCTTTTTGCAGCGTACACCGCGTGGGCGTATGGCG
ACGACGCGGGCGTGGAATCACTTTGGCATAACGCCGCCAGAAATGCCGTAA

Domains

Predicted by InterProScan.

Holliday junction DNA helicase RuvB P-loop domain (RuvB_N)

(23-181)

RuvB AAA lid domain (AAA_lid_4)

(184-257)

RuvB C-terminal winged helix domain (WHD_RuvB)

(259-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q32HA1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ruvB Bacillus subtilis subsp. subtilis str. 168

60.486

97.917

0.592
  ruvB Streptococcus pneumoniae TIGR4

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae R6

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae D39

58.934

94.94

0.56
  ruvB Synechocystis sp. PCC 6803

53.251

96.131

0.512
  ruvB Helicobacter pylori 26695

52.038

94.94

0.494

Multiple sequence alignment