Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ABXJ10_RS20335 Genome accession   NZ_CP159639
Coordinates   4234437..4235870 (+) Length   477 a.a.
NCBI ID   WP_000809051.1    Uniprot ID   A0A0H2VDV8
Organism   Escherichia coli strain Z226-7     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4229437..4240870
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABXJ10_RS20305 (ABXJ10_20305) yrbG 4229995..4230972 (+) 978 WP_022646167.1 calcium/sodium antiporter -
  ABXJ10_RS20310 (ABXJ10_20310) kdsD 4230986..4231972 (+) 987 WP_001295557.1 arabinose-5-phosphate isomerase KdsD -
  ABXJ10_RS20315 (ABXJ10_20315) kdsC 4231993..4232559 (+) 567 WP_000030016.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  ABXJ10_RS20320 (ABXJ10_20320) lptC 4232556..4233131 (+) 576 WP_000030537.1 LPS export ABC transporter periplasmic protein LptC -
  ABXJ10_RS20325 (ABXJ10_20325) lptA 4233100..4233657 (+) 558 WP_000669785.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  ABXJ10_RS20330 (ABXJ10_20330) lptB 4233664..4234389 (+) 726 WP_000224099.1 LPS export ABC transporter ATP-binding protein -
  ABXJ10_RS20335 (ABXJ10_20335) rpoN/rpoN1 4234437..4235870 (+) 1434 WP_000809051.1 RNA polymerase factor sigma-54 Machinery gene
  ABXJ10_RS20340 (ABXJ10_20340) hpf 4235893..4236180 (+) 288 WP_001176599.1 ribosome hibernation promoting factor -
  ABXJ10_RS20345 (ABXJ10_20345) ptsN 4236298..4236789 (+) 492 WP_000183676.1 PTS IIA-like nitrogen regulatory protein PtsN -
  ABXJ10_RS20350 (ABXJ10_20350) rapZ 4236835..4237689 (+) 855 WP_000243741.1 RNase adapter RapZ -
  ABXJ10_RS20355 (ABXJ10_20355) npr 4237686..4237958 (+) 273 WP_000216791.1 PTS phosphocarrier protein NPr -
  ABXJ10_RS20360 (ABXJ10_20360) yrbL 4238171..4238803 (+) 633 WP_000620399.1 PhoP regulatory network protein YrbL -
  ABXJ10_RS20365 (ABXJ10_20365) mtgA 4238800..4239528 (-) 729 WP_000047098.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  ABXJ10_RS20370 (ABXJ10_20370) elbB 4239525..4240178 (-) 654 WP_001302019.1 isoprenoid biosynthesis glyoxalase ElbB -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 53993.79 Da        Isoelectric Point: 4.3639

>NTDB_id=913685 ABXJ10_RS20335 WP_000809051.1 4234437..4235870(+) (rpoN/rpoN1) [Escherichia coli strain Z226-7]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESNPLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPE
ELPLDASWDTIYTAGTPSGTSGDYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARLIISDHLDLLANHDFRTLMRVTR
LKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDVLVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIR
SNLQDAKWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=913685 ABXJ10_RS20335 WP_000809051.1 4234437..4235870(+) (rpoN/rpoN1) [Escherichia coli strain Z226-7]
ATGAAGCAAGGTTTGCAACTCAGGCTTAGCCAACAACTGGCGATGACGCCACAGCTCCAACAGGCAATTCGTCTGTTGCA
GTTGTCGACGCTGGAACTTCAGCAGGAGCTACAGCAGGCGCTGGAGAGTAATCCGCTGCTTGAGCAAATCGACACTCATG
AAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACACTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAA
GAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGA
CGAGCTGCCGGTCTATCAGGGCGAAACGACGCAGACCTTGCAGGATTACCTGATGTGGCAGGTCGAGTTGACACCGTTTT
CCGACACTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGACACTGGTTATCTGACTGTCCCGCTGGAAGAT
ATTCTCGAAAGTATGGGCGATGAAGAGATTGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCC
GGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGCCTGCTGATCCAACTCTCCCAATTCGATAAAACCACGCCGTGGCTGG
AAGAGGCCAGACTGATTATTAGCGATCATCTCGATCTGTTAGCCAATCACGACTTCCGCACTTTAATGCGCGTCACGCGT
CTGAAAGAAGATGTGCTGAAAGAAGCCGTCAATCTGATCCAGTCGCTCGATCCGCGCCCCGGGCAGTCGATCCAGACTGG
CGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAACTCAACAGTGACAGCA
TTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGC
AGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGTCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTAT
CGTTGAACAGCAGCAAGCCTTCTTTGAGCAAGGTGAAGAATATATGAAACCGATGGTACTGGCCGATATCGCCCAGGCTG
TCGAAATGCATGAATCGACGATATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAG
TATTTCTTTTCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCGCTGGTGAAGAAATT
AATCGCGGCGGAAAACCCAGCGAAACCGTTGAGCGACAGCAAGTTAACCTCTTTGCTGTCGGAACAAGGTATCATGGTGG
CACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAGCTCGTTTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2VDV8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

44.223

100

0.465