Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   SR852_RS11145 Genome accession   NZ_CP140161
Coordinates   2194572..2195201 (+) Length   209 a.a.
NCBI ID   WP_003183693.1    Uniprot ID   Q8VQ77
Organism   Bacillus licheniformis strain ATCC 14580     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2189572..2200201
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SR852_RS11110 (SR852_11110) aroE 2190214..2191050 (+) 837 WP_003183706.1 shikimate dehydrogenase -
  SR852_RS11115 (SR852_11115) yhbY 2191044..2191334 (+) 291 WP_003183704.1 ribosome assembly RNA-binding protein YhbY -
  SR852_RS11120 (SR852_11120) - 2191353..2191922 (+) 570 WP_003183702.1 nicotinate-nucleotide adenylyltransferase -
  SR852_RS11125 (SR852_11125) yqeK 2191912..2192475 (+) 564 WP_003183701.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  SR852_RS11130 (SR852_11130) rsfS 2192489..2192845 (+) 357 WP_003183699.1 ribosome silencing factor -
  SR852_RS11135 (SR852_11135) - 2192842..2193597 (+) 756 WP_011198132.1 class I SAM-dependent DNA methyltransferase -
  SR852_RS11140 (SR852_11140) comER 2193667..2194488 (-) 822 WP_011198131.1 late competence protein ComER -
  SR852_RS11145 (SR852_11145) comEA 2194572..2195201 (+) 630 WP_003183693.1 helix-hairpin-helix domain-containing protein Machinery gene
  SR852_RS11150 (SR852_11150) - 2195270..2195839 (+) 570 WP_003183690.1 ComE operon protein 2 -
  SR852_RS11155 (SR852_11155) - 2195843..2198230 (+) 2388 WP_009327865.1 DNA internalization-related competence protein ComEC/Rec2 -
  SR852_RS11160 (SR852_11160) - 2198255..2198386 (-) 132 WP_003183686.1 YqzM family protein -
  SR852_RS11165 (SR852_11165) holA 2198616..2199659 (+) 1044 WP_009327866.1 DNA polymerase III subunit delta -
  SR852_RS11170 (SR852_11170) rpsT 2199721..2199987 (-) 267 WP_003183682.1 30S ribosomal protein S20 -

Sequence

Protein


Download         Length: 209 a.a.        Molecular weight: 22468.42 Da        Isoelectric Point: 5.1890

>NTDB_id=913227 SR852_RS11145 WP_003183693.1 2194572..2195201(+) (comEA) [Bacillus licheniformis strain ATCC 14580]
MTDWLKQYKWHAAGGVALVLIISAAFMLLSGKRETSSGLSIPEEASAQTYDKKEEVKREKSAGKEAVIIDLKGAVKNPGV
YQMKEGDRVHDVLKKAGGTEKKADQKQINLAAVLQDGMVVYIPFEGEEAADSFSKAGSRADGASVDIVNINTASSEELQA
IPGIGPSKAEAVIEYREENGPFHTVEDITNVSGIGEKSFERIKSAITVK

Nucleotide


Download         Length: 630 bp        

>NTDB_id=913227 SR852_RS11145 WP_003183693.1 2194572..2195201(+) (comEA) [Bacillus licheniformis strain ATCC 14580]
TTGACAGATTGGCTAAAACAATATAAATGGCATGCTGCGGGAGGCGTTGCACTCGTCTTGATCATCAGCGCTGCCTTCAT
GCTGTTAAGCGGAAAGCGTGAAACTTCATCCGGCCTCTCCATTCCCGAGGAGGCTTCTGCACAGACGTATGACAAGAAGG
AGGAGGTGAAACGGGAAAAAAGCGCGGGAAAAGAGGCGGTTATCATCGATTTGAAAGGCGCTGTGAAAAATCCGGGCGTC
TATCAAATGAAAGAGGGAGACAGGGTCCACGATGTATTGAAAAAAGCAGGCGGCACCGAGAAAAAAGCGGATCAAAAGCA
AATTAACCTTGCAGCCGTTTTGCAGGACGGTATGGTGGTTTACATTCCATTTGAGGGGGAAGAGGCTGCTGATTCTTTCT
CGAAAGCGGGTTCAAGGGCAGATGGCGCTTCCGTCGATATCGTCAATATCAATACGGCTTCCTCTGAGGAGCTTCAGGCG
ATTCCCGGCATCGGCCCTTCAAAAGCGGAAGCGGTTATCGAATACCGCGAGGAGAACGGACCGTTTCACACGGTAGAAGA
CATAACAAACGTTTCGGGAATTGGAGAAAAGTCTTTTGAAAGAATAAAATCTGCAATCACGGTAAAGTAA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(147-207)

SLBB domain (SLBB)

(69-123)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8VQ77

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

51.402

100

0.526