Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   L6432_RS07540 Genome accession   NZ_AP025339
Coordinates   1524998..1526032 (-) Length   344 a.a.
NCBI ID   WP_035338372.1    Uniprot ID   -
Organism   Bacillus paralicheniformis strain J25TS1     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1519998..1531032
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L6432_RS07490 tasA 1520068..1520862 (-) 795 WP_020452167.1 biofilm matrix protein TasA -
  L6432_RS07495 sipW 1520935..1521519 (-) 585 WP_035338370.1 signal peptidase I SipW -
  L6432_RS07500 tapA 1521516..1522244 (-) 729 WP_020452169.1 amyloid fiber anchoring/assembly protein TapA -
  L6432_RS07505 - 1522522..1522842 (+) 321 WP_023855188.1 YqzG/YhdC family protein -
  L6432_RS07510 - 1522872..1523054 (-) 183 WP_020452171.1 YqzE family protein -
  L6432_RS07515 comGG 1523143..1523508 (-) 366 WP_025811163.1 competence type IV pilus minor pilin ComGG -
  L6432_RS07520 comGF 1523520..1524002 (-) 483 WP_230588654.1 competence type IV pilus minor pilin ComGF -
  L6432_RS07525 comGE 1523917..1524264 (-) 348 WP_025811161.1 competence type IV pilus minor pilin ComGE -
  L6432_RS07530 comGD 1524248..1524685 (-) 438 WP_020452175.1 competence type IV pilus minor pilin ComGD -
  L6432_RS07535 comGC 1524691..1524984 (-) 294 WP_020452176.1 competence type IV pilus major pilin ComGC Machinery gene
  L6432_RS07540 comGB 1524998..1526032 (-) 1035 WP_035338372.1 competence type IV pilus assembly protein ComGB Machinery gene
  L6432_RS07545 comGA 1526022..1527086 (-) 1065 WP_025811157.1 competence type IV pilus ATPase ComGA Machinery gene
  L6432_RS07550 - 1527244..1528083 (-) 840 WP_035338373.1 STAS domain-containing protein -
  L6432_RS07560 - 1528325..1528702 (-) 378 WP_003183482.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  L6432_RS07565 - 1528913..1529158 (+) 246 WP_020452180.1 DUF2626 domain-containing protein -
  L6432_RS07570 - 1529194..1529832 (-) 639 WP_020452181.1 MBL fold metallo-hydrolase -
  L6432_RS07575 - 1529988..1530161 (+) 174 WP_003183486.1 DUF2759 domain-containing protein -
  L6432_RS07580 - 1530222..1530536 (-) 315 WP_020452182.1 MTH1187 family thiamine-binding protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 39807.12 Da        Isoelectric Point: 9.6575

>NTDB_id=91316 L6432_RS07540 WP_035338372.1 1524998..1526032(-) (comGB) [Bacillus paralicheniformis strain J25TS1]
MKIKMKWPVREQADILKKLGDMMKSGYTILDALSMLKLQLNERRRADLTFAMEKLTEGRPVFQVLEMISFHKDAVTIVYF
AEQHGNLPYAFRQSGELLHRKIAQTEKLKKAARYPLFLVLTVCVIISIMKSAIIPQFSAIYESMNVETSFATTLIFSVFD
HFYLFIAGMMLIAAALSLYYLCSFRHKPPEDKMTFLIRIPLLGQTFKLFNSYFLSLQLSNLLQAGLSVYDSLKAFESQPF
LSFHKNEAKRLIERLKQGESLEQMLAGHPFYENDLAKAVAHGQLNGHLYRELYSYSQFLIERLERKAEKWTGLIQPLIYG
LTAAMILILYLSMLLPMYQMMNQL

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=91316 L6432_RS07540 WP_035338372.1 1524998..1526032(-) (comGB) [Bacillus paralicheniformis strain J25TS1]
ATGAAGATTAAAATGAAGTGGCCCGTCCGGGAACAGGCGGACATTCTGAAAAAACTCGGAGACATGATGAAGAGCGGATA
TACGATTTTGGATGCTTTAAGTATGCTGAAACTGCAATTGAATGAAAGGCGAAGAGCAGATCTGACATTTGCGATGGAGA
AGCTGACAGAAGGGCGACCTGTGTTTCAAGTGCTTGAAATGATTTCATTTCATAAGGATGCTGTTACCATTGTTTACTTT
GCCGAACAGCACGGGAATTTGCCGTATGCCTTTCGGCAGAGCGGAGAGCTGCTTCACCGCAAGATCGCTCAAACCGAAAA
ACTAAAGAAAGCCGCCAGATATCCCTTGTTTTTAGTTTTAACGGTCTGCGTCATTATCTCTATTATGAAATCGGCAATCA
TCCCGCAATTTTCCGCGATCTATGAATCAATGAACGTTGAAACGTCTTTTGCAACGACCCTCATCTTTTCGGTTTTTGAT
CATTTTTATCTTTTTATCGCAGGAATGATGTTGATTGCCGCCGCTTTATCCCTCTATTATCTGTGTTCATTTCGCCATAA
ACCTCCAGAAGACAAAATGACCTTTCTCATTAGAATCCCCTTGCTGGGCCAAACCTTCAAACTATTCAACAGTTATTTTT
TATCGCTCCAATTGAGCAATCTTTTGCAAGCTGGTCTGTCTGTATATGACAGTTTAAAAGCATTTGAAAGCCAGCCGTTT
TTATCATTTCATAAAAATGAAGCGAAGCGCCTGATCGAGCGGCTGAAACAAGGGGAGAGTCTGGAGCAGATGCTGGCCGG
GCATCCGTTTTATGAAAATGATCTGGCGAAGGCTGTTGCTCACGGCCAATTAAACGGACACTTATACAGAGAGCTGTATT
CATACAGTCAATTTTTGATTGAACGGCTTGAACGGAAAGCAGAAAAATGGACAGGTTTGATCCAGCCATTGATTTACGGG
CTAACAGCGGCGATGATATTAATTCTTTATTTGTCTATGCTTTTGCCAATGTATCAAATGATGAACCAGTTATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(216-336)

Type II secretion system (T2SS), protein F (T2SSF)

(16-135)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Bacillus subtilis subsp. subtilis str. 168

57.276

93.895

0.538

Multiple sequence alignment