Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   SR914_RS07960 Genome accession   NZ_CP140157
Coordinates   1748407..1750008 (+) Length   533 a.a.
NCBI ID   WP_003060568.1    Uniprot ID   B7X452
Organism   Comamonas testosteroni strain DSMZ 14576     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1743407..1755008
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SR914_RS07940 (SR914_07940) - 1745185..1745889 (+) 705 WP_003060555.1 SPOR domain-containing protein -
  SR914_RS07945 (SR914_07945) - 1746045..1746695 (+) 651 WP_003060558.1 thiol:disulfide interchange protein DsbA/DsbL -
  SR914_RS07950 (SR914_07950) lptA 1746826..1747482 (+) 657 WP_029158548.1 lipopolysaccharide transport periplasmic protein LptA -
  SR914_RS07955 (SR914_07955) lptB 1747479..1748261 (+) 783 WP_003060565.1 LPS export ABC transporter ATP-binding protein -
  SR914_RS07960 (SR914_07960) rpoN/rpoN1 1748407..1750008 (+) 1602 WP_003060568.1 RNA polymerase factor sigma-54 Machinery gene
  SR914_RS07965 (SR914_07965) - 1750012..1751601 (-) 1590 WP_003060572.1 S41 family peptidase -
  SR914_RS07970 (SR914_07970) - 1751867..1752790 (-) 924 WP_019043169.1 aspartyl/asparaginyl beta-hydroxylase domain-containing protein -
  SR914_RS07975 (SR914_07975) - 1752924..1754156 (-) 1233 WP_003060577.1 DUF711 family protein -
  SR914_RS07980 (SR914_07980) - 1754221..1754559 (-) 339 WP_003060580.1 tRNA-binding protein -

Sequence

Protein


Download         Length: 533 a.a.        Molecular weight: 59043.34 Da        Isoelectric Point: 4.6654

>NTDB_id=913140 SR914_RS07960 WP_003060568.1 1748407..1750008(+) (rpoN/rpoN1) [Comamonas testosteroni strain DSMZ 14576]
MKPGLSLRVSQHLSLTPQLQQSIQLLQLSTLELAQEVEQMLVDNPFLERAGDEAETAGDAPLDVASSASEYSTSDSIHAE
KEASQAQLATETVAEAPSPETPETLNWEADGMDGQDGEWGGESSSEWDTPSASSSTRNRDGDDETDAIDLAREHQSLTDF
LHRQALSLHLGAADTAALRFLIESLSDDGYLEDSLQELAQSLAGDDLEEREELEQHFAIALKLLQSLEPVGVGARDLAEC
LQLQLRDLIRTAEDEGTTEAHMNRLLLAQAICAQPMDLLARRDLRKLTSLCGAREEQLREAMALIARLEPRPGRRFVDVE
RHIVVPDVIVRPVRAGSGRAEFSVQLNPEVMPRLRVHDIYAGVLRRHKNSDGHAALQQQLQEARWFIKNIQQRFDTILRV
SEAIVERQKNFFVHGELAMRPLVLRDIADTLGLHESTISRVTTAKYMATPQGTFELKYFFGSGLGTETGGSASSTAVRAL
IKQFVDAEDARKPLSDAKLADMLKEQGIECARRTVAKYREQLKIPTTTLRKAL

Nucleotide


Download         Length: 1602 bp        

>NTDB_id=913140 SR914_RS07960 WP_003060568.1 1748407..1750008(+) (rpoN/rpoN1) [Comamonas testosteroni strain DSMZ 14576]
ATGAAGCCAGGTCTCTCGCTACGCGTTTCGCAGCATTTATCACTGACGCCGCAGCTGCAGCAATCCATTCAGCTGCTGCA
GCTCTCCACTCTGGAGCTGGCGCAGGAAGTCGAGCAGATGCTGGTCGACAACCCTTTTCTGGAGCGTGCGGGCGATGAGG
CCGAAACTGCGGGAGATGCTCCTTTAGATGTAGCTAGTAGCGCCAGTGAATACAGTACTTCAGATAGTATTCATGCTGAA
AAAGAAGCGTCACAAGCGCAATTAGCTACTGAAACAGTAGCGGAAGCACCGAGCCCCGAAACCCCGGAAACCCTGAACTG
GGAGGCCGACGGCATGGACGGCCAGGACGGCGAATGGGGCGGCGAATCCAGCAGCGAGTGGGATACGCCCTCGGCCAGCA
GCTCCACGCGCAACCGCGATGGCGATGACGAAACCGATGCCATCGATCTGGCGCGCGAGCACCAGAGCCTGACCGACTTT
CTCCATCGTCAGGCGCTGAGCCTGCATCTGGGCGCGGCAGACACGGCGGCGCTGCGCTTTCTCATCGAGTCGCTCAGCGA
CGACGGCTATCTCGAGGATTCCCTGCAGGAGCTGGCGCAGAGCCTGGCCGGCGACGATCTGGAAGAGCGCGAGGAGCTGG
AGCAGCACTTTGCCATTGCGCTGAAGCTGCTGCAAAGCCTGGAGCCGGTGGGCGTGGGCGCGCGCGATCTGGCCGAGTGC
CTGCAGCTGCAGCTGCGCGACCTGATCCGCACGGCCGAGGACGAGGGCACGACCGAGGCGCATATGAACCGGCTGCTGCT
GGCGCAGGCCATCTGCGCGCAGCCCATGGACTTGCTGGCGCGGCGCGATCTGCGCAAGCTCACCAGCCTGTGCGGCGCGC
GCGAGGAACAGCTGCGCGAGGCCATGGCCCTGATCGCCAGGCTGGAGCCGCGTCCGGGGCGCCGCTTTGTCGACGTGGAG
CGCCATATCGTGGTGCCCGATGTCATCGTGCGCCCGGTGCGGGCCGGCTCGGGCAGGGCAGAATTTTCCGTGCAGCTCAA
CCCCGAGGTCATGCCGCGTCTGCGCGTGCACGACATCTATGCCGGAGTGCTGCGTCGCCACAAGAACAGCGACGGTCATG
CGGCGCTGCAGCAGCAGCTGCAGGAGGCGCGCTGGTTCATCAAGAACATCCAGCAGCGCTTCGACACCATCTTGCGTGTT
TCCGAAGCCATCGTGGAGCGGCAGAAGAACTTCTTTGTGCATGGCGAGCTGGCCATGCGCCCGCTCGTGCTACGCGATAT
CGCCGACACGCTGGGCCTGCACGAGTCCACCATCAGCCGCGTGACCACGGCCAAGTACATGGCCACGCCCCAAGGTACTT
TCGAGCTCAAGTATTTCTTCGGTTCGGGCCTGGGCACGGAAACCGGCGGCAGTGCCTCCAGCACGGCCGTACGTGCGCTG
ATCAAACAGTTTGTCGATGCCGAAGATGCCAGAAAGCCGCTGTCGGACGCCAAGCTGGCCGACATGCTCAAGGAGCAGGG
CATTGAGTGTGCGCGCCGCACCGTCGCCAAATACCGCGAGCAGCTCAAGATACCGACGACCACGCTGCGCAAGGCGCTCT
AA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(375-531)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(150-360)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7X452

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

49.54

100

0.505