Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   L6432_RS04345 Genome accession   NZ_AP025339
Coordinates   844464..844892 (+) Length   142 a.a.
NCBI ID   WP_020451590.1    Uniprot ID   A0A6N2GRD1
Organism   Bacillus paralicheniformis strain J25TS1     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 839464..849892
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L6432_RS04315 - 839720..840328 (+) 609 WP_023857599.1 flavin reductase family protein -
  L6432_RS04320 wrbA 840530..841144 (+) 615 WP_026579653.1 NAD(P)H:quinone oxidoreductase -
  L6432_RS04325 - 841455..841595 (+) 141 WP_023857597.1 hypothetical protein -
  L6432_RS04330 - 841588..842421 (+) 834 WP_020451587.1 prohibitin family protein -
  L6432_RS04335 - 842510..843271 (+) 762 WP_026579652.1 hypothetical protein -
  L6432_RS04340 - 843331..844170 (+) 840 WP_026579651.1 thymidylate synthase -
  L6432_RS04345 nucA/comI 844464..844892 (+) 429 WP_020451590.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  L6432_RS04350 lexA 845091..845714 (-) 624 WP_020451591.1 transcriptional repressor LexA -
  L6432_RS04355 yneA 845866..846177 (+) 312 WP_020451592.1 cell division suppressor protein YneA -
  L6432_RS04360 - 846196..846843 (+) 648 WP_020451593.1 YneB family resolvase-like protein -
  L6432_RS04365 - 846909..847142 (+) 234 WP_020451594.1 DUF896 domain-containing protein -
  L6432_RS04370 tkt 847306..849309 (+) 2004 WP_023857593.1 transketolase -

Sequence

Protein


Download         Length: 142 a.a.        Molecular weight: 15420.36 Da        Isoelectric Point: 7.8950

>NTDB_id=91309 L6432_RS04345 WP_020451590.1 844464..844892(+) (nucA/comI) [Bacillus paralicheniformis strain J25TS1]
MIKKWAVHLLFSALVLLGLSGGAAYSPQHAEGAARYDDVLYFPASRYPETGAHISDAIKAGHSDVCTIERSGADKRRQES
LKGIPTKPGFDRDEWPMAMCEEGGKGASVRYVSSSDNRGAGSWVGNRLSDYADGTRILFIVR

Nucleotide


Download         Length: 429 bp        

>NTDB_id=91309 L6432_RS04345 WP_020451590.1 844464..844892(+) (nucA/comI) [Bacillus paralicheniformis strain J25TS1]
ATGATCAAAAAATGGGCGGTTCATCTGCTGTTTTCCGCATTGGTGCTGCTTGGGCTTTCGGGAGGGGCTGCATATTCTCC
TCAGCATGCCGAAGGCGCTGCAAGGTATGATGACGTATTGTATTTTCCGGCATCGCGCTATCCTGAAACAGGTGCTCATA
TAAGCGATGCGATCAAAGCGGGCCATTCAGATGTCTGCACAATTGAAAGATCTGGAGCGGATAAGCGCCGTCAGGAATCA
TTAAAGGGGATTCCGACTAAGCCGGGCTTTGACCGTGACGAATGGCCGATGGCCATGTGTGAAGAAGGGGGAAAAGGAGC
GTCGGTCAGATATGTCAGCTCATCGGATAACCGCGGAGCCGGCTCCTGGGTCGGGAACAGGCTGAGCGATTACGCTGACG
GGACGAGAATTTTGTTTATCGTTCGATAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(45-137)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6N2GRD1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

59.524

88.732

0.528

Multiple sequence alignment