Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   L6411_RS09160 Genome accession   NZ_AP025333
Coordinates   1904063..1905427 (-) Length   454 a.a.
NCBI ID   WP_024532694.1    Uniprot ID   -
Organism   Streptococcus ruminantium strain GUT-189     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1899063..1910427
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L6411_RS09135 (GUT189_17720) gltX 1899456..1900910 (-) 1455 WP_024532985.1 glutamate--tRNA ligase -
  L6411_RS09140 (GUT189_17730) - 1901066..1901755 (-) 690 WP_237374112.1 LiaF transmembrane domain-containing protein -
  L6411_RS09145 (GUT189_17740) - 1901761..1902207 (-) 447 WP_155964794.1 LytTR family DNA-binding domain-containing protein -
  L6411_RS09150 (GUT189_17750) - 1902614..1903333 (-) 720 WP_024532696.1 TIGR00266 family protein -
  L6411_RS09155 (GUT189_17760) - 1903444..1903938 (-) 495 WP_155962049.1 beta-class carbonic anhydrase -
  L6411_RS09160 (GUT189_17770) radA 1904063..1905427 (-) 1365 WP_024532694.1 DNA repair protein RadA Machinery gene
  L6411_RS09165 (GUT189_17780) - 1905945..1906388 (-) 444 WP_024532693.1 dUTP diphosphatase -
  L6411_RS09170 (GUT189_17790) - 1906826..1907680 (-) 855 WP_156010973.1 helix-turn-helix domain-containing protein -
  L6411_RS09175 (GUT189_17800) - 1908009..1908629 (+) 621 WP_237373217.1 hypothetical protein -
  L6411_RS09180 (GUT189_17810) - 1908999..1909292 (+) 294 WP_120172339.1 bacteriocin immunity protein -

Sequence

Protein


Download         Length: 454 a.a.        Molecular weight: 49734.26 Da        Isoelectric Point: 6.8567

>NTDB_id=91259 L6411_RS09160 WP_024532694.1 1904063..1905427(-) (radA) [Streptococcus ruminantium strain GUT-189]
MIAKKKTTFVCQSCEYHSPKYLGRCPNCGSWSSFVEEVAVTEVKNERVSLTGEKTRPMKLNEVSSIQVSRTKTSMEEFNR
VLGGGVVPGSLVLIGGDPGIGKSTLLLQVSTQLSTIGTVLYVSGEESAQQIKLRAERLGDIDSEFYLYAETNMQSIRKEI
EKIKPDFLIIDSIQTIMSPDISSVQGSVSQVREVTNELMQIAKTNNIATFIVGHMTKEGTLAGPRTLEHMVDTVLYFEGE
RQHTFRILRAVKNRFGSTNEIGIFEMQSQGLVEVLNPSEVFLEERLDGATGSAIVVTMEGTRPILAEVQALVTPTMFGNA
KRTTTGLDFNRASLIMAVLEKRAGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVALASSYKDKPTNPQECFIGEIGLTGEI
RRVNRIEQRINEAAKLGFTKVYAPKNSLTGIKVPKEIMAVGVTTIGEVLQKVFN

Nucleotide


Download         Length: 1365 bp        

>NTDB_id=91259 L6411_RS09160 WP_024532694.1 1904063..1905427(-) (radA) [Streptococcus ruminantium strain GUT-189]
ATCATCGCTAAGAAAAAAACAACCTTTGTCTGTCAGTCCTGCGAGTACCACTCGCCCAAATACCTAGGGCGCTGTCCCAA
CTGTGGTTCTTGGTCGTCCTTTGTAGAGGAAGTGGCAGTTACAGAAGTTAAGAATGAGCGAGTCAGCTTGACAGGCGAGA
AGACCCGACCAATGAAGCTAAATGAAGTTTCCTCTATCCAAGTGTCTCGTACAAAGACTAGTATGGAAGAGTTCAATCGC
GTTCTGGGAGGAGGTGTAGTGCCCGGGAGTTTGGTTCTTATTGGTGGAGATCCGGGTATAGGCAAGTCCACCTTGCTCTT
GCAGGTTTCAACTCAGCTATCAACTATTGGCACAGTTCTTTATGTATCAGGAGAAGAGTCTGCCCAACAAATCAAGCTAC
GAGCAGAGCGCTTAGGCGATATTGACAGCGAGTTTTATCTCTATGCAGAGACCAATATGCAGAGTATCCGCAAGGAGATT
GAAAAAATCAAGCCGGATTTTTTGATTATTGATTCTATTCAGACGATTATGAGTCCGGATATTTCTAGCGTGCAGGGATC
AGTCAGTCAGGTGCGTGAAGTAACCAATGAGCTCATGCAGATTGCCAAGACTAATAATATCGCAACCTTTATTGTCGGTC
ACATGACCAAGGAAGGAACTCTTGCAGGACCGCGTACTCTAGAACACATGGTGGATACGGTGCTTTATTTTGAGGGAGAG
CGCCAGCATACCTTCCGCATCTTGCGAGCGGTCAAAAATCGTTTTGGATCAACCAATGAGATCGGAATTTTTGAAATGCA
GTCTCAGGGTTTGGTAGAGGTTCTCAATCCTAGCGAAGTCTTTTTGGAAGAACGTTTGGATGGAGCGACAGGTTCTGCCA
TTGTTGTTACCATGGAGGGAACCAGACCTATCTTAGCAGAAGTACAAGCCTTGGTGACTCCGACCATGTTTGGTAATGCT
AAGCGGACGACAACAGGACTAGACTTCAATCGGGCTAGCTTAATTATGGCGGTTTTGGAAAAAAGGGCAGGTCTACTTTT
ACAAAATCAAGATGCTTACCTAAAATCAGCAGGTGGTGTCAAGTTAGATGAGCCGGCGATTGATCTTGCGGTAGCAGTTG
CCCTCGCTTCCAGCTACAAGGATAAGCCCACCAATCCTCAGGAGTGTTTTATCGGGGAAATCGGTCTGACAGGAGAAATA
CGCCGTGTCAATCGGATCGAACAGCGCATCAATGAAGCGGCTAAGTTGGGATTTACAAAAGTCTATGCTCCAAAAAATTC
CTTGACAGGTATCAAGGTCCCTAAAGAAATTATGGCTGTAGGAGTGACAACAATTGGTGAAGTTTTGCAAAAAGTATTTA
ATTAG

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(63-219)

Subunit ChlI of Mg-chelatase (ChlI)

(362-426)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae Rx1

90.265

99.559

0.899
  radA Streptococcus pneumoniae D39

90.265

99.559

0.899
  radA Streptococcus pneumoniae R6

90.265

99.559

0.899
  radA Streptococcus pneumoniae TIGR4

90.265

99.559

0.899
  radA Streptococcus mitis NCTC 12261

90.265

99.559

0.899
  radA Streptococcus mitis SK321

90.044

99.559

0.896
  radA Bacillus subtilis subsp. subtilis str. 168

62.472

99.78

0.623

Multiple sequence alignment