Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   U0040_RS02620 Genome accession   NZ_CP140003
Coordinates   540402..541202 (+) Length   266 a.a.
NCBI ID   WP_010327532.1    Uniprot ID   -
Organism   Acinetobacter johnsonii strain HAMBI_0097     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 535402..546202
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U0040_RS02590 (U0040_02590) - 535613..536029 (+) 417 WP_004983505.1 hypothetical protein -
  U0040_RS02595 (U0040_02595) - 536170..537450 (+) 1281 WP_010327534.1 glycosyltransferase family 4 protein -
  U0040_RS02600 (U0040_02600) - 537643..538194 (+) 552 WP_004697666.1 phosphatase PAP2 family protein -
  U0040_RS02605 (U0040_02605) iscX 538285..538485 (+) 201 WP_004697668.1 Fe-S cluster assembly protein IscX -
  U0040_RS02610 (U0040_02610) ndk 538596..539027 (+) 432 WP_004697669.1 nucleoside-diphosphate kinase -
  U0040_RS02615 (U0040_02615) rlmN 539150..540385 (+) 1236 WP_004697671.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  U0040_RS02620 (U0040_02620) pilF 540402..541202 (+) 801 WP_010327532.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  U0040_RS02625 (U0040_02625) - 541193..541969 (+) 777 WP_010327531.1 helix-turn-helix domain-containing protein -
  U0040_RS02630 (U0040_02630) ispG 541988..543109 (+) 1122 WP_010327530.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  U0040_RS02635 (U0040_02635) hisS 543113..544408 (+) 1296 WP_078425672.1 histidine--tRNA ligase -
  U0040_RS02640 (U0040_02640) - 544436..545134 (+) 699 WP_114837678.1 YfgM family protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30049.02 Da        Isoelectric Point: 9.1263

>NTDB_id=912343 U0040_RS02620 WP_010327532.1 540402..541202(+) (pilF) [Acinetobacter johnsonii strain HAMBI_0097]
MKKPVSKLYLALTLGVSVWYLVGCQTVNTKGDPSKAVQVRTQLAAEYIKSNDYDAAKRTLDQALDIDSRDASANMMMGVL
LQREGSPQNVEKAERYFKHAIAAEPKNAQARNNYGTYLYQIGRYNDAIDQLQVAGSTLGYDQRYRALENLGRAYLQVGRV
AEAEAAFKQALQVNSGAYLAMIEMAEISYLRQQNAEATQYYEQFVRAVGEKNLDARALWIGIRIARASHDTMGSQVLVNQ
LRALYPDSQEYKRYLQLQYTTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=912343 U0040_RS02620 WP_010327532.1 540402..541202(+) (pilF) [Acinetobacter johnsonii strain HAMBI_0097]
TTGAAAAAACCCGTTTCTAAACTTTACCTTGCGCTGACACTGGGTGTGTCAGTGTGGTACTTGGTGGGTTGCCAAACCGT
GAATACGAAAGGTGACCCAAGTAAAGCAGTACAAGTTAGAACGCAATTGGCGGCTGAATATATTAAGTCGAATGATTACG
ATGCTGCGAAACGCACATTGGATCAGGCATTAGACATCGACTCTCGTGATGCATCTGCAAATATGATGATGGGCGTTTTG
TTACAGCGTGAAGGCAGTCCACAAAATGTGGAAAAGGCAGAGCGTTATTTTAAGCATGCAATTGCGGCTGAGCCAAAAAA
TGCACAGGCTCGTAACAACTACGGTACGTATTTATATCAAATTGGGCGTTATAATGACGCGATTGATCAACTTCAAGTTG
CAGGTTCAACTTTAGGGTATGATCAACGTTATCGAGCATTGGAGAATTTAGGTCGTGCGTATCTACAAGTAGGTCGAGTG
GCGGAAGCAGAAGCTGCTTTTAAACAAGCCCTACAGGTAAACTCAGGTGCCTATCTTGCCATGATCGAAATGGCGGAAAT
TTCATATTTACGTCAGCAAAATGCTGAAGCTACGCAGTACTATGAACAGTTTGTGCGAGCGGTTGGCGAGAAAAACTTAG
ATGCACGTGCGCTTTGGATTGGGATCCGAATTGCGCGAGCAAGTCATGACACGATGGGTTCGCAAGTACTGGTCAACCAG
CTACGTGCATTGTATCCAGACAGTCAAGAATACAAACGTTATTTGCAATTACAGTACACTACTGAGGCCGTATGGAAGTA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_19)

(89-147)

Tetratricopeptide repeat (TPR_16)

(149-206)

Tetratricopeptide repeat (TPR_8)

(39-68)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

68.284

100

0.688
  pilF Acinetobacter baylyi ADP1

64.286

100

0.643