Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   U0040_RS01500 Genome accession   NZ_CP140003
Coordinates   328500..329237 (+) Length   245 a.a.
NCBI ID   WP_004978082.1    Uniprot ID   N9BLX7
Organism   Acinetobacter johnsonii strain HAMBI_0097     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 323500..334237
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U0040_RS01485 (U0040_01485) ponA 324095..326623 (-) 2529 WP_114836329.1 penicillin-binding protein PBP1a -
  U0040_RS01490 (U0040_01490) - 326789..327847 (+) 1059 Protein_287 pilus assembly protein PilM -
  U0040_RS01495 (U0040_01495) comN 327847..328503 (+) 657 WP_004978083.1 PilN domain-containing protein Machinery gene
  U0040_RS01500 (U0040_01500) pilO 328500..329237 (+) 738 WP_004978082.1 type 4a pilus biogenesis protein PilO Machinery gene
  U0040_RS01505 (U0040_01505) pilP 329237..329764 (+) 528 WP_004978081.1 pilus assembly protein PilP Machinery gene
  U0040_RS01510 (U0040_01510) pilQ 329784..331862 (+) 2079 WP_114836328.1 type IV pilus secretin PilQ Machinery gene
  U0040_RS01515 (U0040_01515) aroK 331900..332433 (+) 534 WP_005401350.1 shikimate kinase AroK -
  U0040_RS01520 (U0040_01520) aroB 332464..333549 (+) 1086 WP_005401349.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 245 a.a.        Molecular weight: 27735.28 Da        Isoelectric Point: 5.1834

>NTDB_id=912334 U0040_RS01500 WP_004978082.1 328500..329237(+) (pilO) [Acinetobacter johnsonii strain HAMBI_0097]
MSRDEFEELTSENHAVTKKKMTVDKFFQQFNTLDPNNYGSWPFSVKLTCWIFIILVIGALGYFVVIKPKLDAISNAHAQE
QNLLNEFREKDSKLRNLQQYQVQLQEMEANFNQQLEQLPKETEIPGLVEDINVSGVNSGLKFKNIRLEPEIRQEFFIEQP
ISIEATGDYHSFGSFVSGIAALPRIVTLHDFDISASSNNDKKTDIPQISYSVKAKTYRYVGATATTDVDVKDTKNDKKAD
AGGAK

Nucleotide


Download         Length: 738 bp        

>NTDB_id=912334 U0040_RS01500 WP_004978082.1 328500..329237(+) (pilO) [Acinetobacter johnsonii strain HAMBI_0097]
ATGAGTCGCGATGAATTTGAAGAGTTAACATCAGAAAACCATGCTGTAACCAAGAAGAAAATGACGGTTGATAAGTTTTT
TCAACAGTTCAATACTTTAGATCCAAACAATTACGGCAGTTGGCCCTTTTCAGTCAAATTGACTTGTTGGATTTTTATCA
TTTTAGTAATTGGGGCATTGGGCTATTTTGTCGTGATTAAGCCGAAGCTTGATGCGATTTCAAATGCACATGCACAAGAA
CAAAATTTACTGAATGAGTTTCGTGAAAAAGATTCAAAATTACGTAATTTGCAGCAATATCAAGTGCAATTGCAAGAAAT
GGAAGCCAATTTTAATCAGCAGTTAGAACAGCTGCCGAAAGAAACTGAAATTCCTGGCTTGGTTGAAGATATCAACGTGA
GCGGCGTAAATTCTGGATTAAAGTTTAAAAATATTCGTTTAGAACCAGAAATTCGCCAAGAGTTCTTTATCGAACAGCCG
ATTTCCATTGAAGCGACGGGTGATTATCATTCTTTTGGTTCATTTGTCAGTGGAATTGCAGCTTTACCGCGTATTGTTAC
CTTGCATGACTTCGATATCTCAGCATCAAGTAACAATGATAAAAAGACGGATATTCCGCAAATTTCATATAGTGTAAAAG
CGAAAACCTACCGTTATGTCGGGGCAACGGCTACAACGGATGTTGATGTAAAAGATACAAAAAATGATAAGAAAGCCGAT
GCGGGAGGTGCCAAGTAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(74-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB N9BLX7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Acinetobacter baumannii D1279779

74.89

92.653

0.694
  comO Acinetobacter baylyi ADP1

72.398

90.204

0.653