Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   U0024_RS00525 Genome accession   NZ_CP139994
Coordinates   115727..117112 (-) Length   461 a.a.
NCBI ID   WP_302092755.1    Uniprot ID   -
Organism   Comamonas testosteroni strain HAMBI_0403     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 110727..122112
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U0024_RS00495 (U0024_00495) - 110757..111521 (+) 765 WP_304349836.1 glycosyltransferase family 25 protein -
  U0024_RS00500 (U0024_00500) - 111511..112644 (-) 1134 WP_304349837.1 glycosyltransferase -
  U0024_RS00505 (U0024_00505) - 112709..113674 (+) 966 WP_304349838.1 glycosyltransferase family 32 protein -
  U0024_RS00510 (U0024_00510) - 113720..113929 (-) 210 WP_003059801.1 zinc-finger domain-containing protein -
  U0024_RS00515 (U0024_00515) - 114071..115009 (-) 939 WP_302092752.1 branched-chain amino acid transaminase -
  U0024_RS00520 (U0024_00520) - 115091..115546 (-) 456 WP_302092753.1 glycerate kinase -
  U0024_RS00525 (U0024_00525) radA 115727..117112 (-) 1386 WP_302092755.1 DNA repair protein RadA Machinery gene
  U0024_RS00535 (U0024_00535) - 117676..118983 (-) 1308 WP_302092756.1 Glu/Leu/Phe/Val family dehydrogenase -
  U0024_RS00540 (U0024_00540) - 119193..120116 (+) 924 WP_302092757.1 fumarylacetoacetate hydrolase family protein -
  U0024_RS00545 (U0024_00545) - 120324..120833 (+) 510 WP_304349839.1 nuclear transport factor 2 family protein -
  U0024_RS00550 (U0024_00550) - 120861..121976 (-) 1116 WP_302092759.1 PPK2 family polyphosphate kinase -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 48043.37 Da        Isoelectric Point: 7.6670

>NTDB_id=912297 U0024_RS00525 WP_302092755.1 115727..117112(-) (radA) [Comamonas testosteroni strain HAMBI_0403]
MAKEKTTFTCNACGGTSPRWLGKCPGCGAWNTLVETVADSASGGKNRLSQPQGYAGLANAQPVTPLSAIEAQDVARTPSG
IEELDRVLGGGVVEGGVVLIGGDPGIGKSTLLLQAMDALHRIGLPTLYVTGEESGAQVALRSRRLGLDNSQVNVLAEIQL
EKILATVEATQPAVVVIDSIQTVYSDQLSSAPGSVAQVRECAAHLTRAAKTTGITIILVGHVTKDGALAGPRVLEHMVDT
VLYFEGDTHSNFRLVRAIKNRFGAVNEIGVFAMTEKGLKGVTNPSAIFLSQHSEPVPGSCVLVTLEGTRPMLVEIQALVD
QGGPAARRLSVGLDRDRLAMLLAVLNRHAGVACADQDVFVNAVGGVRINEPAADLAVMLAITSSLRGKSLPKGFIAFGEV
GLAGEVRPAPRGQERLKEAAKLGFTIAVVPKANAPKKPIAGLAIHAVERVDEAINIVRGIG

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=912297 U0024_RS00525 WP_302092755.1 115727..117112(-) (radA) [Comamonas testosteroni strain HAMBI_0403]
ATGGCCAAAGAGAAAACCACTTTCACCTGCAACGCCTGTGGCGGCACCAGCCCGCGCTGGCTGGGCAAATGCCCGGGCTG
CGGCGCCTGGAACACACTGGTCGAGACCGTGGCCGACTCCGCCTCGGGCGGCAAGAACCGCCTGAGCCAGCCTCAGGGCT
ATGCAGGTCTGGCCAATGCCCAGCCCGTCACCCCGCTGTCGGCCATCGAGGCCCAGGATGTGGCGCGCACGCCCAGCGGC
ATCGAGGAGCTGGACCGCGTGCTGGGCGGCGGCGTGGTGGAAGGCGGCGTGGTGTTGATCGGGGGAGATCCCGGAATAGG
TAAGTCCACACTCCTTTTGCAGGCCATGGATGCATTGCATCGTATCGGCCTGCCAACGCTCTATGTAACGGGCGAGGAAA
GCGGTGCCCAGGTGGCGCTGCGCTCGCGCCGGCTGGGGCTGGACAACAGCCAGGTCAATGTGCTGGCCGAAATCCAGCTG
GAGAAAATCCTCGCCACCGTGGAGGCCACCCAGCCCGCCGTGGTGGTGATTGACTCGATCCAGACGGTGTATTCGGACCA
GCTCTCCTCAGCTCCGGGATCCGTGGCCCAGGTGCGCGAATGCGCGGCCCATCTGACGCGCGCGGCCAAGACCACGGGCA
TCACCATCATTTTGGTGGGCCATGTGACCAAGGACGGCGCGCTGGCCGGCCCGCGCGTGCTCGAGCACATGGTGGACACG
GTGCTCTACTTCGAAGGCGACACGCACAGCAACTTCCGCCTAGTGCGCGCCATCAAGAACCGCTTCGGTGCCGTCAACGA
GATCGGCGTCTTCGCGATGACGGAAAAAGGCCTCAAGGGCGTGACCAACCCCAGCGCCATCTTTCTGAGTCAGCACAGCG
AGCCCGTGCCCGGCAGCTGCGTGCTGGTGACGCTGGAGGGCACGCGCCCCATGCTGGTGGAAATCCAGGCCCTGGTGGAC
CAGGGCGGCCCGGCCGCACGCCGCCTCTCGGTCGGCCTGGACCGCGACCGGCTGGCCATGCTGCTGGCCGTGCTCAACCG
CCATGCGGGCGTGGCCTGCGCCGACCAGGATGTCTTCGTCAATGCCGTGGGCGGCGTGCGTATCAATGAGCCTGCGGCTG
ACCTTGCCGTGATGTTGGCAATCACTTCCAGTCTGCGCGGCAAGTCGCTGCCCAAAGGCTTTATTGCGTTTGGCGAGGTT
GGTCTGGCCGGAGAGGTGCGCCCAGCACCTCGCGGCCAGGAGCGGCTCAAGGAGGCGGCAAAGTTGGGATTCACTATCGC
CGTGGTCCCCAAGGCCAATGCACCCAAGAAACCCATCGCCGGCCTGGCCATCCATGCCGTGGAGCGCGTGGATGAGGCCA
TCAACATCGTGCGCGGCATTGGTTGA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(68-226)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

49.02

99.566

0.488
  radA Streptococcus mitis NCTC 12261

47.598

99.349

0.473
  radA Streptococcus pneumoniae D39

49.425

94.36

0.466
  radA Streptococcus pneumoniae TIGR4

49.425

94.36

0.466
  radA Streptococcus mitis SK321

49.425

94.36

0.466
  radA Streptococcus pneumoniae R6

49.425

94.36

0.466
  radA Streptococcus pneumoniae Rx1

49.425

94.36

0.466