Detailed information    

insolico Bioinformatically predicted

Overview

Name   endA   Type   Machinery gene
Locus tag   U0447_RS03960 Genome accession   NZ_CP139861
Coordinates   753401..754225 (-) Length   274 a.a.
NCBI ID   WP_050232570.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain 16H2017-2     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 748401..759225
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U0447_RS03955 - 751779..753110 (-) 1332 WP_000390039.1 hemolysin family protein -
  U0447_RS03960 endA 753401..754225 (-) 825 WP_050232570.1 DNA-entry nuclease EndA Machinery gene
  U0447_RS03965 - 754264..754452 (-) 189 WP_001813390.1 DNA-directed RNA polymerase subunit beta -
  U0447_RS03970 murA 754445..755728 (-) 1284 WP_000358035.1 UDP-N-acetylglucosamine 1-carboxyvinyltransferase -
  U0447_RS03975 - 755752..755982 (-) 231 WP_001809307.1 DUF1146 family protein -
  U0447_RS03980 sepM 756040..757077 (-) 1038 WP_000727277.1 SepM family pheromone-processing serine protease Regulator
  U0447_RS03985 coaD 757061..757549 (-) 489 WP_001280736.1 pantetheine-phosphate adenylyltransferase -
  U0447_RS03990 rsmD 757539..758078 (-) 540 WP_044788620.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  U0447_RS03995 asnA 758143..759135 (-) 993 WP_044791888.1 aspartate--ammonia ligase -

Sequence

Protein


Download         Length: 274 a.a.        Molecular weight: 29962.68 Da        Isoelectric Point: 10.1700

>NTDB_id=911404 U0447_RS03960 WP_050232570.1 753401..754225(-) (endA) [Streptococcus pneumoniae strain 16H2017-2]
MNKKTRQTLIGLLVLLLLSTGSYYIKQMQSAPNSPKTKLNQKKQASEAPSQALAESVLTDAVKSQIKGSLEWNGSGAFIV
NGNKTNLDAKVSSKPYADNKTKTVGKETVPTVANALLSKATRQYKNRKETGNGSTSWTPPGWHQVKNLKGSYTHAVDRGH
LLGYALIGGLDGFDASTSNPKNIAVQTAWANQAQAEYSTGQNYYESKVRKALDQNKRVRYRVTLYYASNEDLVPSASQIE
AKSSDGELEFNVLVPNVQKGLQLDYRTGEVTVTQ

Nucleotide


Download         Length: 825 bp        

>NTDB_id=911404 U0447_RS03960 WP_050232570.1 753401..754225(-) (endA) [Streptococcus pneumoniae strain 16H2017-2]
ATGAACAAAAAAACAAGACAGACACTAATCGGACTGCTAGTGTTATTGCTTTTGTCTACAGGGAGCTATTATATCAAGCA
GATGCAGTCGGCACCTAATAGTCCTAAAACCAAGCTTAATCAGAAAAAACAAGCGTCTGAAGCTCCTAGTCAAGCATTGG
CAGAGAGTGTCTTAACAGACGCAGTCAAGAGTCAAATAAAGGGGAGTCTGGAGTGGAATGGCTCAGGTGCTTTTATCGTC
AATGGTAATAAAACAAATCTAGATGCCAAGGTTTCAAGTAAGCCCTACGCTGACAATAAAACAAAGACAGTGGGCAAGGA
AACTGTTCCAACCGTAGCTAATGCCCTCTTGTCTAAGGCCACTCGTCAGTACAAGAATCGTAAAGAAACTGGGAATGGTT
CAACTTCTTGGACTCCTCCAGGTTGGCATCAGGTCAAGAATCTAAAGGGCTCTTATACCCATGCAGTCGATAGAGGTCAT
TTGTTAGGCTATGCCTTAATCGGTGGTTTGGATGGTTTTGATGCCTCAACAAGCAATCCTAAAAACATTGCTGTTCAGAC
AGCCTGGGCAAATCAGGCACAAGCCGAGTATTCGACTGGTCAAAACTACTATGAAAGCAAGGTGCGTAAAGCCTTGGACC
AAAACAAGCGTGTCCGTTACCGTGTAACCCTTTACTACGCTTCAAACGAGGATTTAGTTCCCTCAGCTTCACAGATTGAA
GCCAAGTCTTCGGATGGAGAATTGGAATTCAATGTTCTAGTTCCCAATGTTCAAAAGGGACTTCAACTGGATTACCGAAC
TGGAGAAGTAACTGTAACTCAGTAA

Domains

Predicted by InterproScan.

DNA/RNA non-specific endonuclease (Endonuclease_NS)

(75-247)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  endA Streptococcus pneumoniae Rx1

98.905

100

0.989
  endA Streptococcus pneumoniae D39

98.905

100

0.989
  endA Streptococcus pneumoniae R6

98.905

100

0.989
  endA Streptococcus pneumoniae TIGR4

98.905

100

0.989