Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   U0448_RS05190 Genome accession   NZ_CP139860
Coordinates   1018421..1019719 (-) Length   432 a.a.
NCBI ID   WP_000867609.1    Uniprot ID   A0A0T8HBK0
Organism   Streptococcus pneumoniae strain 21011     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1013421..1024719
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U0448_RS05170 rplI 1014576..1015028 (-) 453 WP_000864220.1 50S ribosomal protein L9 -
  U0448_RS05175 - 1015025..1016998 (-) 1974 WP_001864876.1 DHH family phosphoesterase -
  U0448_RS05180 hpf 1017134..1017682 (-) 549 WP_000599104.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  U0448_RS05185 comFC/cflB 1017762..1018424 (-) 663 WP_000649971.1 ComF family protein Machinery gene
  U0448_RS05190 comFA/cflA 1018421..1019719 (-) 1299 WP_000867609.1 DEAD/DEAH box helicase Machinery gene
  U0448_RS05195 - 1019775..1020410 (+) 636 WP_000395739.1 YigZ family protein -
  U0448_RS05200 cysK 1020695..1021615 (+) 921 WP_000029866.1 cysteine synthase A -
  U0448_RS05205 - 1021854..1022204 (-) 351 Protein_984 IS66 family transposase -
  U0448_RS05210 - 1022204..1022497 (+) 294 Protein_985 transposase -
  U0448_RS05215 tnpB 1022520..1022870 (-) 351 WP_000586658.1 IS66 family insertion sequence element accessory protein TnpB -
  U0448_RS05220 tsf 1023117..1024157 (-) 1041 WP_000808063.1 translation elongation factor Ts -

Sequence

Protein


Download         Length: 432 a.a.        Molecular weight: 49505.44 Da        Isoelectric Point: 8.5992

>NTDB_id=911346 U0448_RS05190 WP_000867609.1 1018421..1019719(-) (comFA/cflA) [Streptococcus pneumoniae strain 21011]
MKVNLDYLGRLFTENELTEEERQLAEKLPAMRKEKGKLFCQRCNSTILEEWYLPIGAYYCRECLLMKRVRSDQTLYYFPQ
EDFPKQDVLKWRGQLTPFQEKVSEGLLQAVDKQKPTLVHAVTGAGKTEMIYQVVAKVINAGGAVCLASPRIDVCLELYKR
LQQDFSCGIALLHGESEPYFRTPLVVATTHQLLKFYQAFDLLIVDEVDAFPYVDNPTLYHAVKNSVKENGLRIFLTATST
NELDKKVRLGELKRLSLPRRFHGNPLIIPKPIWLSDFNRYLDKNRLSPKLKSYIEKQRKTAYPLLIFASEIKKGEQLAEI
LQEQFPNEKIGFVSSVTEDRLEQVQAFRDGELTILISTTILERGVTFPCVDVFVVEANHRLFTKSSLIQIGGRVGRSMDR
PTGDLLFFHDGLNASIKKAIKEIQMMNKEAGL

Nucleotide


Download         Length: 1299 bp        

>NTDB_id=911346 U0448_RS05190 WP_000867609.1 1018421..1019719(-) (comFA/cflA) [Streptococcus pneumoniae strain 21011]
ATGAAAGTAAATTTAGATTATCTCGGTCGTTTATTTACTGAGAATGAATTAACAGAAGAAGAACGTCAGTTGGCGGAGAA
ACTTCCAGCAATGAGAAAGGAGAAGGGGAAACTTTTCTGTCAACGTTGTAATAGTACTATTCTAGAAGAATGGTATTTGC
CCATCGGTGCTTACTATTGTCGAGAGTGCTTGCTGATGAAGCGAGTCAGAAGTGATCAAACTTTATACTATTTTCCGCAG
GAGGATTTTCCGAAGCAAGATGTTCTCAAATGGCGCGGCCAATTAACTCCTTTTCAAGAGAAGGTGTCAGAGGGACTGCT
TCAAGCAGTAGACAAGCAAAAGCCAACCTTAGTTCATGCGGTAACAGGAGCTGGAAAGACAGAAATGATTTATCAAGTAG
TGGCTAAAGTGATCAATGCGGGTGGTGCAGTGTGTTTGGCTAGTCCTCGCATAGATGTTTGTTTGGAGCTGTACAAGCGC
CTGCAACAGGATTTTTCTTGCGGGATAGCTTTGCTACATGGAGAATCGGAACCTTATTTTCGAACACCACTAGTTGTTGC
AACAACCCATCAGTTATTGAAGTTTTATCAAGCTTTTGATTTGCTGATAGTGGATGAAGTAGATGCTTTTCCTTATGTTG
ATAATCCCACGCTTTACCACGCTGTCAAGAATAGTGTAAAGGAGAATGGATTGAGAATCTTTTTAACAGCGACTTCGACC
AATGAGTTAGATAAAAAGGTCCGTTTAGGAGAACTAAAAAGACTGAGTTTACCGAGACGGTTTCATGGAAATCCGTTGAT
TATTCCAAAACCAATTTGGTTATCGGATTTTAATCGCTACTTAGACAAGAATCGTTTGTCACCAAAGTTAAAGTCCTATA
TTGAGAAGCAGAGAAAGACAGCTTATCCGTTACTCATTTTTGCTTCAGAAATTAAGAAAGGGGAGCAGTTAGCAGAAATC
TTACAGGAGCAATTTCCAAATGAGAAAATTGGCTTTGTATCTTCTGTAACAGAGGATCGATTAGAGCAAGTACAAGCTTT
TCGAGATGGAGAACTGACAATACTTATCAGTACGACAATCTTGGAGCGTGGAGTTACCTTCCCTTGTGTGGATGTTTTCG
TAGTAGAGGCCAATCATCGTTTGTTTACCAAGTCTAGTTTGATTCAGATTGGTGGACGAGTTGGACGAAGCATGGATAGA
CCGACAGGAGATTTGCTTTTCTTCCATGATGGGTTAAATGCTTCAATCAAGAAGGCGATTAAGGAAATTCAGATGATGAA
TAAGGAGGCTGGTCTATGA

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(293-396)

Type III restriction enzyme, res subunit (ResIII)

(94-238)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0T8HBK0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus pneumoniae Rx1

99.537

100

0.995
  comFA/cflA Streptococcus pneumoniae D39

99.537

100

0.995
  comFA/cflA Streptococcus pneumoniae R6

99.537

100

0.995
  comFA/cflA Streptococcus pneumoniae TIGR4

99.306

100

0.993
  comFA/cflA Streptococcus mitis NCTC 12261

93.519

100

0.935
  comFA/cflA Streptococcus mitis SK321

92.361

100

0.924
  comFA Lactococcus lactis subsp. cremoris KW2

52.206

94.444

0.493
  comFA Latilactobacillus sakei subsp. sakei 23K

38.979

99.769

0.389