Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ABU973_RS10270 Genome accession   NZ_CP159170
Coordinates   1887806..1889239 (+) Length   477 a.a.
NCBI ID   WP_000809051.1    Uniprot ID   A0A0H2VDV8
Organism   Escherichia coli strain 10035776-3     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1882806..1894239
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABU973_RS10240 (ABU973_10240) yrbG 1883364..1884341 (+) 978 WP_000922881.1 calcium/sodium antiporter -
  ABU973_RS10245 (ABU973_10245) kdsD 1884355..1885341 (+) 987 WP_001295557.1 arabinose-5-phosphate isomerase KdsD -
  ABU973_RS10250 (ABU973_10250) kdsC 1885362..1885928 (+) 567 WP_000030006.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  ABU973_RS10255 (ABU973_10255) lptC 1885925..1886500 (+) 576 WP_000030537.1 LPS export ABC transporter periplasmic protein LptC -
  ABU973_RS10260 (ABU973_10260) lptA 1886469..1887026 (+) 558 WP_000669785.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  ABU973_RS10265 (ABU973_10265) lptB 1887033..1887758 (+) 726 WP_000224099.1 LPS export ABC transporter ATP-binding protein -
  ABU973_RS10270 (ABU973_10270) rpoN/rpoN1 1887806..1889239 (+) 1434 WP_000809051.1 RNA polymerase factor sigma-54 Machinery gene
  ABU973_RS10275 (ABU973_10275) hpf 1889262..1889549 (+) 288 WP_001176599.1 ribosome hibernation promoting factor -
  ABU973_RS10280 (ABU973_10280) ptsN 1889667..1890158 (+) 492 WP_000183676.1 PTS IIA-like nitrogen regulatory protein PtsN -
  ABU973_RS10285 (ABU973_10285) rapZ 1890204..1891058 (+) 855 WP_000243741.1 RNase adapter RapZ -
  ABU973_RS10290 (ABU973_10290) npr 1891055..1891327 (+) 273 WP_000216791.1 PTS phosphocarrier protein NPr -
  ABU973_RS10295 (ABU973_10295) yrbL 1891541..1892173 (+) 633 WP_001544960.1 PhoP regulatory network protein YrbL -
  ABU973_RS10300 (ABU973_10300) mtgA 1892170..1892898 (-) 729 WP_001544962.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  ABU973_RS10305 (ABU973_10305) elbB 1892895..1893548 (-) 654 WP_001300411.1 isoprenoid biosynthesis glyoxalase ElbB -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 53993.79 Da        Isoelectric Point: 4.3639

>NTDB_id=911074 ABU973_RS10270 WP_000809051.1 1887806..1889239(+) (rpoN/rpoN1) [Escherichia coli strain 10035776-3]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESNPLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPE
ELPLDASWDTIYTAGTPSGTSGDYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARLIISDHLDLLANHDFRTLMRVTR
LKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDVLVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIR
SNLQDAKWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=911074 ABU973_RS10270 WP_000809051.1 1887806..1889239(+) (rpoN/rpoN1) [Escherichia coli strain 10035776-3]
ATGAAGCAAGGTTTGCAACTCAGGCTTAGCCAACAACTGGCGATGACGCCACAGCTCCAACAGGCAATTCGTCTGTTGCA
GTTGTCGACGCTGGAACTTCAGCAGGAGCTACAGCAGGCGCTGGAGAGTAATCCGCTGCTTGAGCAAATCGACACTCATG
AAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACACTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAA
GAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCAGGTACACCATCCGGCACCAGCGGTGACTACATTGACGA
CGAGCTGCCGGTCTATCAGGGCGAAACGACGCAGACCTTGCAGGATTACCTGATGTGGCAGGTCGAGCTGACACCGTTTT
CCGATACTGACCGCGCCATTGCTACCTCTATCGTCGATGCCGTTGATGACACCGGTTATCTGACTGTCCCGCTGGAAGAT
ATTCTCGAAAGTATGGGCGATGAAGAGATCGACATCGACGAGGTTGAAGCTGTCCTTAAGCGGATCCAACGGTTTGATCC
GGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGCCTGCTGATCCAACTCTCCCAATTCGATAAAACTACGCCGTGGCTGG
AAGAGGCCAGACTGATCATTAGCGATCATCTCGATCTGTTAGCCAATCACGACTTCCGCACTTTAATGCGCGTCACGCGT
CTGAAAGAAGATGTGCTGAAAGAAGCCGTCAATCTGATCCAGTCGCTCGATCCGCGCCCCGGGCAGTCGATCCAGACTGG
CGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAACTCAACAGTGACAGCA
TTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGATAGCCAGTTTATCCGC
AGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGTCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTAT
CGTTGAACAGCAGCAAGCCTTCTTTGAGCAAGGTGAAGAATATATGAAACCGATGGTACTGGCCGATATCGCCCAGGCTG
TCGAAATGCATGAATCGACGATATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAG
TATTTCTTTTCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCGCTGGTGAAGAAATT
AATCGCGGCGGAAAACCCAGCGAAACCGTTGAGCGACAGCAAGTTAACCTCTTTGCTGTCGGAACAAGGTATCATGGTGG
CACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAGCTCGTTTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2VDV8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

44.223

100

0.465