Detailed information
Bioinformatically predicted
Overview
| Name | nucA/comI | Type | Machinery gene |
| Locus tag | R6I59_RS13940 | Genome accession | NZ_CP139744 |
| Coordinates | 2651977..2652387 (-) | Length | 136 a.a. |
| NCBI ID | WP_009967785.1 | Uniprot ID | A0A6I4D881 |
| Organism | Bacillus subtilis isolate FELIX_MS255 | ||
| Function | cleavage of dsDNA into ssDNA (predicted from homology) DNA processing |
||
Related MGE
Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.
Gene-MGE association summary
| MGE type | MGE coordinates | Gene coordinates | Relative position | Distance (bp) |
|---|---|---|---|---|
| Prophage | 2647343..2698833 | 2651977..2652387 | within | 0 |
Gene organization within MGE regions
Location: 2647343..2698833
| Locus tag | Gene name | Coordinates (strand) | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| R6I59_RS13915 (R6I59_13915) | yqeF | 2647510..2648241 (-) | 732 | WP_003229964.1 | SGNH/GDSL hydrolase family protein | - |
| R6I59_RS13920 (R6I59_13920) | cwlH | 2648493..2649245 (-) | 753 | WP_003229963.1 | N-acetylmuramoyl-L-alanine amidase CwlH | - |
| R6I59_RS13925 (R6I59_13925) | yqeD | 2649432..2650058 (+) | 627 | WP_003229962.1 | TVP38/TMEM64 family protein | - |
| R6I59_RS13930 (R6I59_13930) | gnd | 2650077..2650970 (-) | 894 | WP_003229961.1 | phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) | - |
| R6I59_RS13935 (R6I59_13935) | yqeB | 2651222..2651944 (+) | 723 | WP_010886572.1 | hypothetical protein | - |
| R6I59_RS13940 (R6I59_13940) | nucA/comI | 2651977..2652387 (-) | 411 | WP_009967785.1 | sporulation-specific Dnase NucB | Machinery gene |
| R6I59_RS13945 (R6I59_13945) | - | 2652583..2652933 (+) | 351 | Protein_2700 | sigma-70 family RNA polymerase sigma factor | - |
| R6I59_RS13950 (R6I59_13950) | spoIVCA | 2652961..2654421 (-) | 1461 | WP_223257626.1 | site-specific DNA recombinase SpoIVCA | - |
| R6I59_RS13955 (R6I59_13955) | - | 2654379..2654557 (-) | 179 | Protein_2702 | hypothetical protein | - |
| R6I59_RS13960 (R6I59_13960) | arsC | 2654912..2655331 (-) | 420 | WP_004398596.1 | thioredoxin-dependent arsenate reductase | - |
| R6I59_RS13965 (R6I59_13965) | acr3 | 2655343..2656383 (-) | 1041 | WP_004398718.1 | arsenite efflux transporter Acr3 | - |
| R6I59_RS13970 (R6I59_13970) | arsK | 2656406..2656846 (-) | 441 | WP_003229954.1 | ArsI/CadI family heavy metal resistance metalloenzyme | - |
| R6I59_RS13975 (R6I59_13975) | arsR | 2656907..2657224 (-) | 318 | WP_004399122.1 | arsenical resistance operon transcriptional regulator ArsR | - |
| R6I59_RS13980 (R6I59_13980) | yqcI | 2657596..2658360 (-) | 765 | WP_004398670.1 | YqcI/YcgG family protein | - |
| R6I59_RS13985 (R6I59_13985) | rapE | 2658803..2659930 (+) | 1128 | WP_004398842.1 | response regulator aspartate phosphatase RapE | - |
| R6I59_RS13990 (R6I59_13990) | phrE | 2659920..2660054 (+) | 135 | WP_004398770.1 | phosphatase RapE inhibitor PhrE | - |
| R6I59_RS13995 (R6I59_13995) | - | 2660164..2660322 (+) | 159 | WP_003245945.1 | hypothetical protein | - |
| R6I59_RS14000 (R6I59_14000) | yqcG | 2660692..2662287 (+) | 1596 | WP_004399034.1 | LXG family T7SS effector endonuclease toxin YqcG | - |
| R6I59_RS14005 (R6I59_14005) | yqcF | 2662302..2662880 (+) | 579 | WP_009967790.1 | type VII secretion system immunity protein YqcF | - |
| R6I59_RS14010 (R6I59_14010) | - | 2662998..2663144 (+) | 147 | WP_009967791.1 | hypothetical protein | - |
| R6I59_RS14015 (R6I59_14015) | - | 2663141..2663503 (-) | 363 | WP_003229947.1 | hypothetical protein | - |
| R6I59_RS14020 (R6I59_14020) | - | 2663519..2663998 (-) | 480 | WP_004399085.1 | hypothetical protein | - |
| R6I59_RS14025 (R6I59_14025) | cwlA | 2664163..2664981 (-) | 819 | WP_003229946.1 | N-acetylmuramoyl-L-alanine amidase CwlA | - |
| R6I59_RS14030 (R6I59_14030) | skhD | 2665026..2665448 (-) | 423 | WP_003246208.1 | phage holin family protein | - |
| R6I59_RS14035 (R6I59_14035) | xepA | 2665493..2666386 (-) | 894 | WP_003246010.1 | phage-like element PBSX protein XepA | - |
| R6I59_RS14040 (R6I59_14040) | yqcE | 2666474..2666638 (-) | 165 | WP_003229944.1 | XkdX family protein | - |
| R6I59_RS14045 (R6I59_14045) | yqcD | 2666635..2666970 (-) | 336 | WP_009967793.1 | XkdW family protein | - |
| R6I59_RS14050 (R6I59_14050) | yqcC | 2666980..2668080 (-) | 1101 | WP_003229943.1 | pyocin knob domain-containing protein | - |
| R6I59_RS14055 (R6I59_14055) | - | 2668083..2668355 (-) | 273 | WP_003229942.1 | hypothetical protein | - |
| R6I59_RS14060 (R6I59_14060) | yqcA | 2668352..2668930 (-) | 579 | WP_003229941.1 | YmfQ family protein | - |
| R6I59_RS14065 (R6I59_14065) | yqbT | 2668914..2669960 (-) | 1047 | WP_003229940.1 | baseplate J/gp47 family protein | - |
| R6I59_RS14070 (R6I59_14070) | yqbS | 2669953..2670378 (-) | 426 | WP_004398572.1 | DUF2634 domain-containing protein | - |
| R6I59_RS14075 (R6I59_14075) | yqbR | 2670391..2670654 (-) | 264 | WP_003229938.1 | DUF2577 family protein | - |
| R6I59_RS14080 (R6I59_14080) | yqbQ | 2670651..2671631 (-) | 981 | WP_004398524.1 | hypothetical protein | - |
| R6I59_RS14085 (R6I59_14085) | yqbP | 2671644..2672303 (-) | 660 | WP_004398548.1 | LysM peptidoglycan-binding domain-containing protein | - |
| R6I59_RS14090 (R6I59_14090) | yqbO | 2672296..2677053 (-) | 4758 | WP_003246092.1 | phage tail tape measure protein | - |
| R6I59_RS14095 (R6I59_14095) | - | 2677056..2677193 (-) | 138 | WP_003229934.1 | hypothetical protein | - |
| R6I59_RS14100 (R6I59_14100) | - | 2677235..2677684 (-) | 450 | WP_003229933.1 | phage tail assembly chaperone | - |
| R6I59_RS14105 (R6I59_14105) | txpA | 2677830..2678009 (+) | 180 | WP_004398662.1 | type I toxin-antitoxin system toxin TxpA | - |
| R6I59_RS14110 (R6I59_14110) | bsrH | 2678389..2678478 (+) | 90 | WP_075058862.1 | type I toxin-antitoxin system toxin BsrH | - |
| R6I59_RS14115 (R6I59_14115) | yqbM | 2678732..2679175 (-) | 444 | WP_003229930.1 | phage tail tube protein | - |
| R6I59_RS14120 (R6I59_14120) | yqbK | 2679178..2680578 (-) | 1401 | WP_003229929.1 | phage tail sheath family protein | - |
| R6I59_RS14125 (R6I59_14125) | - | 2680579..2680770 (-) | 192 | WP_010886574.1 | hypothetical protein | - |
| R6I59_RS14130 (R6I59_14130) | yqbJ | 2680767..2681204 (-) | 438 | WP_003229927.1 | phage tail terminator family protein | - |
| R6I59_RS14135 (R6I59_14135) | yqbI | 2681217..2681720 (-) | 504 | WP_003246050.1 | HK97 gp10 family phage protein | - |
| R6I59_RS14140 (R6I59_14140) | yqbH | 2681717..2682079 (-) | 363 | WP_003229925.1 | YqbH/XkdH family protein | - |
| R6I59_RS14145 (R6I59_14145) | gkpG | 2682076..2682471 (-) | 396 | WP_004398566.1 | DUF3199 family protein | - |
| R6I59_RS14150 (R6I59_14150) | yqbF | 2682475..2682786 (-) | 312 | WP_003229923.1 | YqbF domain-containing protein | - |
| R6I59_RS14155 (R6I59_14155) | skdG | 2682797..2683732 (-) | 936 | WP_003229922.1 | phage major capsid protein | - |
| R6I59_RS14160 (R6I59_14160) | yqbD | 2683751..2684719 (-) | 969 | WP_003229921.1 | XkdF-like putative serine protease domain-containing protein | - |
| R6I59_RS14165 (R6I59_14165) | - | 2684752..2685405 (-) | 654 | WP_003229920.1 | hypothetical protein | - |
| R6I59_RS14170 (R6I59_14170) | yqbB | 2685446..2686363 (-) | 918 | WP_004398748.1 | phage head morphogenesis protein | - |
| R6I59_RS14175 (R6I59_14175) | yqbA | 2686360..2687892 (-) | 1533 | WP_004398894.1 | phage portal protein | - |
| R6I59_RS14180 (R6I59_14180) | stmB | 2687896..2689191 (-) | 1296 | WP_003229917.1 | PBSX family phage terminase large subunit | - |
| R6I59_RS14185 (R6I59_14185) | terS | 2689184..2689903 (-) | 720 | WP_003229916.1 | phage terminase small subunit | - |
| R6I59_RS14190 (R6I59_14190) | - | 2689971..2690435 (-) | 465 | WP_004398685.1 | hypothetical protein | - |
| R6I59_RS14195 (R6I59_14195) | yqaQ | 2690579..2691034 (-) | 456 | WP_004398775.1 | hypothetical protein | - |
| R6I59_RS14200 (R6I59_14200) | - | 2691232..2692161 (+) | 930 | WP_003229913.1 | hypothetical protein | - |
| R6I59_RS14205 (R6I59_14205) | yqaO | 2692235..2692441 (-) | 207 | WP_003229912.1 | XtrA/YqaO family protein | - |
| R6I59_RS14210 (R6I59_14210) | yqaN | 2692523..2692951 (-) | 429 | WP_009967809.1 | RusA family crossover junction endodeoxyribonuclease | - |
| R6I59_RS14215 (R6I59_14215) | - | 2693047..2693196 (-) | 150 | WP_003229910.1 | hypothetical protein | - |
| R6I59_RS14220 (R6I59_14220) | sknM | 2693187..2694128 (-) | 942 | WP_075058863.1 | ATP-binding protein | - |
| R6I59_RS14225 (R6I59_14225) | yqaL | 2694010..2694687 (-) | 678 | WP_010886575.1 | DnaD domain-containing protein | - |
| R6I59_RS14230 (R6I59_14230) | recT | 2694763..2695617 (-) | 855 | WP_003229907.1 | recombinase RecT | - |
| R6I59_RS14235 (R6I59_14235) | yqaJ | 2695620..2696579 (-) | 960 | WP_004398673.1 | YqaJ viral recombinase family protein | - |
| R6I59_RS14240 (R6I59_14240) | yqaI | 2696685..2696879 (-) | 195 | WP_003229905.1 | YqaI family protein | - |
| R6I59_RS14245 (R6I59_14245) | - | 2696839..2697012 (-) | 174 | WP_119123069.1 | hypothetical protein | - |
| R6I59_RS14250 (R6I59_14250) | sknH | 2697009..2697266 (-) | 258 | WP_003245994.1 | YqaH family protein | - |
| R6I59_RS14255 (R6I59_14255) | yqaG | 2697263..2697832 (-) | 570 | WP_004398626.1 | helix-turn-helix domain-containing protein | - |
| R6I59_RS14260 (R6I59_14260) | - | 2697906..2698046 (-) | 141 | WP_003229902.1 | hypothetical protein | - |
| R6I59_RS14265 (R6I59_14265) | yqaF | 2698076..2698306 (-) | 231 | WP_004398958.1 | helix-turn-helix transcriptional regulator | - |
| R6I59_RS14270 (R6I59_14270) | sknR | 2698483..2698833 (+) | 351 | WP_004398704.1 | transcriptional regulator SknR | - |
Sequence
Protein
Download Length: 136 a.a. Molecular weight: 14967.97 Da Isoelectric Point: 5.1853
>NTDB_id=910825 R6I59_RS13940 WP_009967785.1 2651977..2652387(-) (nucA/comI) [Bacillus subtilis isolate FELIX_MS255]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ
Nucleotide
Download Length: 411 bp
>NTDB_id=910825 R6I59_RS13940 WP_009967785.1 2651977..2652387(-) (nucA/comI) [Bacillus subtilis isolate FELIX_MS255]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGGGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGTGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGTACCAGAGTGCTGTTTA
TTGTGCAGTAG
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGGGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGTGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGTACCAGAGTGCTGTTTA
TTGTGCAGTAG
Similar proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| nucA/comI | Bacillus subtilis subsp. subtilis str. 168 |
62.609 |
84.559 |
0.529 |