Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoS   Type   Regulator
Locus tag   ABU963_RS12225 Genome accession   NZ_CP159137
Coordinates   2171396..2172388 (-) Length   330 a.a.
NCBI ID   WP_000081550.1    Uniprot ID   A0A370V765
Organism   Escherichia coli strain 158274-3     
Function   regulation of chitinases (predicted from homology)   
Competence regulation

Genomic Context

Location: 2166396..2177388
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABU963_RS12200 (ABU963_12200) ygbN 2167051..2168415 (+) 1365 WP_000104439.1 GntP family transporter -
  ABU963_RS12205 (ABU963_12205) - 2168454..2168690 (-) 237 WP_000562984.1 non-oxidative hydroxyarylic acid decarboxylases subunit D -
  ABU963_RS12210 (ABU963_12210) - 2168701..2170128 (-) 1428 WP_000863195.1 non-oxidative hydroxyarylic acid decarboxylases subunit C -
  ABU963_RS12215 (ABU963_12215) - 2170128..2170721 (-) 594 WP_000767707.1 non-oxidative hydroxyarylic acid decarboxylases subunit B -
  ABU963_RS12220 (ABU963_12220) - 2170868..2171275 (+) 408 WP_001208070.1 MarR family transcriptional regulator -
  ABU963_RS12225 (ABU963_12225) rpoS 2171396..2172388 (-) 993 WP_000081550.1 RNA polymerase sigma factor RpoS Regulator
  ABU963_RS12230 (ABU963_12230) nlpD 2172451..2173545 (-) 1095 WP_224692580.1 murein hydrolase activator NlpD -
  ABU963_RS12235 (ABU963_12235) pcm 2173730..2174356 (-) 627 WP_000254708.1 protein-L-isoaspartate O-methyltransferase -
  ABU963_RS12240 (ABU963_12240) surE 2174350..2175111 (-) 762 WP_001472109.1 5'/3'-nucleotidase SurE -
  ABU963_RS12245 (ABU963_12245) truD 2175092..2176141 (-) 1050 WP_000568943.1 tRNA pseudouridine(13) synthase TruD -
  ABU963_RS12250 (ABU963_12250) ispF 2176138..2176617 (-) 480 WP_001219242.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  ABU963_RS12255 (ABU963_12255) ispD 2176617..2177327 (-) 711 WP_000246138.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -

Sequence

Protein


Download         Length: 330 a.a.        Molecular weight: 37972.84 Da        Isoelectric Point: 4.5597

>NTDB_id=910745 ABU963_RS12225 WP_000081550.1 2171396..2172388(-) (rpoS) [Escherichia coli strain 158274-3]
MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEELLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFA
RRALRGDVASRRRMIESNLRLVVKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNERITSVDTPLGGDSEKALLDILAD
EKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLARRFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQ
GLNIEALFRE

Nucleotide


Download         Length: 993 bp        

>NTDB_id=910745 ABU963_RS12225 WP_000081550.1 2171396..2172388(-) (rpoS) [Escherichia coli strain 158274-3]
ATGAGTCAGAATACGCTGAAAGTTCATGATTTAAATGAAGATGCGGAATTTGATGAGAACGGAGTTGAGGTTTTTGACGA
AAAGGCCTTAGTAGAAGAGGAACCCAGTGATAACGATTTGGCCGAAGAGGAACTGTTATCGCAGGGAGCCACACAGCGTG
TGTTGGACGCGACTCAGCTTTACCTTGGTGAGATTGGTTATTCACCACTGTTAACGGCCGAAGAAGAAGTTTATTTTGCG
CGTCGCGCACTGCGTGGAGATGTCGCCTCTCGCCGCCGGATGATCGAGAGTAACTTGCGTCTGGTGGTAAAAATTGCCCG
CCGTTATGGCAATCGTGGTCTGGCGTTGCTGGACCTTATCGAAGAGGGCAACCTGGGGCTGATCCGCGCGGTAGAGAAGT
TTGACCCGGAACGTGGTTTCCGCTTCTCAACATACGCAACCTGGTGGATTCGCCAGACGATTGAACGGGCGATTATGAAC
CAAACCCGTACTATTCGTTTGCCGATTCACATCGTAAAGGAGCTGAACGTTTACCTGCGAACCGCACGTGAGTTGTCCCA
TAAGCTGGACCATGAACCAAGTGCGGAAGAGATCGCAGAGCAACTGGATAAGCCAGTTGATGACGTCAGCCGTATGCTTC
GTCTTAACGAGCGCATTACCTCGGTAGACACCCCGCTGGGTGGTGATTCCGAAAAAGCGTTGCTGGACATCCTGGCCGAT
GAAAAAGAGAACGGTCCGGAAGATACCACGCAAGATGACGATATGAAGCAGAGCATCGTCAAATGGCTGTTCGAGCTGAA
CGCCAAACAGCGTGAAGTGCTGGCACGTCGATTCGGTTTGCTGGGGTACGAAGCGGCAACACTGGAAGATGTAGGTCGTG
AAATTGGCCTCACCCGTGAACGTGTTCGCCAGATTCAGGTTGAAGGCCTGCGCCGTTTGCGCGAAATCCTGCAAACGCAG
GGGCTGAATATCGAAGCGCTGTTCCGCGAGTAA

Domains

Predicted by InterProScan.

Sigma-70 region 3 (Sigma70_r3)

(174-248)

Sigma-70 region 2 (Sigma70_r2)

(94-163)

Sigma-70 factor, region 1.2 (Sigma70_r1_2)

(56-88)

Sigma-70, region 4 (Sigma70_r4)

(262-315)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A370V765

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoS Vibrio cholerae O1 biovar El Tor strain E7946

73.065

97.879

0.715