Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   UM396_RS00535 Genome accession   NZ_CP139723
Coordinates   104226..105599 (+) Length   457 a.a.
NCBI ID   WP_322310698.1    Uniprot ID   -
Organism   Geobacillus subterraneus strain OXR-76     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 99226..110599
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  UM396_RS00515 (UM396_00515) ctsR 99600..100061 (+) 462 WP_063167019.1 transcriptional regulator CtsR -
  UM396_RS00520 (UM396_00520) - 100076..100624 (+) 549 WP_063167018.1 UvrB/UvrC motif-containing protein -
  UM396_RS00525 (UM396_00525) - 100629..101720 (+) 1092 WP_063167017.1 protein arginine kinase -
  UM396_RS00530 (UM396_00530) clpC 101717..104152 (+) 2436 WP_081210601.1 ATP-dependent protease ATP-binding subunit ClpC -
  UM396_RS00535 (UM396_00535) radA 104226..105599 (+) 1374 WP_322310698.1 DNA repair protein RadA Machinery gene
  UM396_RS00540 (UM396_00540) - 105807..106901 (+) 1095 WP_063167014.1 PIN/TRAM domain-containing protein -
  UM396_RS00545 (UM396_00545) ispD 106923..107609 (+) 687 WP_063167013.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  UM396_RS00550 (UM396_00550) ispF 107624..108106 (+) 483 WP_063167012.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  UM396_RS00555 (UM396_00555) gltX 108334..109803 (+) 1470 WP_168368003.1 glutamate--tRNA ligase -

Sequence

Protein


Download         Length: 457 a.a.        Molecular weight: 49291.77 Da        Isoelectric Point: 7.4605

>NTDB_id=910705 UM396_RS00535 WP_322310698.1 104226..105599(+) (radA) [Geobacillus subterraneus strain OXR-76]
MVKKKTKFVCQECGYESAKWLGRCPGCQTWNSFVEEIERAKPAARGAFLHSEPSGPAKPVPITAVTAAQEPRIETDSAEL
NRVLGGGIVKGSLVLIGGDPGIGKSTLLLQTSARLAAAGHTVLYISGEESVKQVKLRAGRLHAESNQLYVLAEADLEYIV
TAIETIQPACVIVDSIQTVYRTDITSAPGSVAQVRECTAELMKIAKTKGIAIFIVGHVTKEGAIAGPRLLEHMVDTVLYF
EGERHHTYRILRAVKNRFGSTNEIGIFEMRDIGLREVENPSEVFLEERSRGAAGSTVVAAMEGTRPVLVEIQALVSPTSF
GNPRRMATGLDHSRVSLLMAVLEKRVGLLLQNQDAYLKVAGGVKLDEPAIDLAVAVSIASSFRDRPTNPADVIIGEVGLT
GEVRRVSRIEQRVQEAVKLGFSRVIVPNNNLTGWQPPAGVNVIGVSHVAEALEHTML

Nucleotide


Download         Length: 1374 bp        

>NTDB_id=910705 UM396_RS00535 WP_322310698.1 104226..105599(+) (radA) [Geobacillus subterraneus strain OXR-76]
ATGGTGAAAAAGAAAACGAAATTTGTTTGCCAAGAATGTGGGTATGAATCAGCCAAATGGCTCGGCCGCTGTCCGGGCTG
CCAAACGTGGAATTCGTTCGTTGAAGAAATTGAGCGAGCGAAACCGGCTGCGCGTGGGGCGTTTCTTCATTCTGAGCCAT
CGGGGCCGGCCAAACCGGTTCCGATTACAGCCGTTACGGCGGCGCAAGAGCCGCGCATTGAAACGGACAGTGCGGAGCTC
AATCGCGTGCTCGGCGGGGGAATCGTCAAAGGCTCGCTTGTCTTAATTGGCGGCGATCCCGGCATCGGCAAGTCGACGTT
ACTGCTGCAAACATCAGCGCGGCTCGCCGCGGCCGGACATACGGTGTTGTATATATCCGGTGAGGAATCGGTGAAGCAAG
TGAAGCTGCGCGCCGGGCGCCTCCATGCCGAGTCCAATCAACTGTATGTGTTAGCGGAGGCGGATTTGGAATATATTGTA
ACAGCGATTGAAACGATTCAGCCTGCCTGTGTGATTGTCGATTCCATTCAGACGGTGTACCGGACGGATATTACGTCGGC
GCCGGGCAGCGTCGCCCAAGTGCGCGAGTGCACCGCTGAATTGATGAAAATCGCCAAGACGAAAGGCATCGCGATTTTCA
TTGTCGGCCATGTGACGAAAGAAGGGGCGATCGCCGGGCCGCGTCTGCTCGAGCATATGGTCGATACGGTTCTTTATTTT
GAAGGGGAACGGCATCATACGTACCGCATTTTGCGGGCGGTGAAAAACCGCTTCGGTTCGACGAATGAAATCGGCATTTT
TGAAATGCGTGACATCGGGCTGCGAGAGGTGGAAAACCCGTCGGAAGTGTTTTTGGAAGAGCGGTCGCGCGGGGCGGCTG
GCTCGACGGTTGTCGCGGCGATGGAAGGGACGCGCCCGGTGCTCGTTGAAATTCAGGCGCTCGTTTCGCCGACGAGTTTC
GGCAATCCAAGGCGGATGGCGACCGGCCTCGACCATAGCCGCGTATCGCTGCTCATGGCCGTGCTTGAAAAGCGGGTCGG
TCTGCTGTTGCAAAACCAGGACGCCTATTTGAAGGTGGCTGGCGGGGTAAAGCTTGACGAACCGGCCATCGATTTGGCGG
TCGCTGTCAGCATCGCCTCGAGTTTCCGCGATCGGCCAACCAACCCGGCCGATGTCATTATCGGTGAGGTCGGCTTGACC
GGGGAGGTGCGCCGTGTTTCCCGCATTGAACAACGCGTGCAAGAGGCTGTCAAATTAGGGTTTTCCCGCGTCATTGTGCC
GAACAACAATTTGACTGGCTGGCAGCCGCCTGCAGGCGTCAACGTGATCGGTGTTTCGCATGTGGCCGAGGCGCTCGAGC
ATACGATGTTATAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(60-222)

Subunit ChlI of Mg-chelatase (ChlI)

(365-430)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

74.179

100

0.742
  radA Streptococcus mitis NCTC 12261

64.459

99.125

0.639
  radA Streptococcus mitis SK321

64.238

99.125

0.637
  radA Streptococcus pneumoniae Rx1

64.018

99.125

0.635
  radA Streptococcus pneumoniae D39

64.018

99.125

0.635
  radA Streptococcus pneumoniae R6

64.018

99.125

0.635
  radA Streptococcus pneumoniae TIGR4

64.018

99.125

0.635