Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   SNE23_RS21485 Genome accession   NZ_CP139483
Coordinates   4136914..4137957 (-) Length   347 a.a.
NCBI ID   WP_048531150.1    Uniprot ID   A0A8T9Z920
Organism   Bacillus sp. RA(2023)     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4131914..4142957
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SNE23_RS21440 (SNE23_21440) - 4132341..4132541 (-) 201 WP_000106081.1 YqzE family protein -
  SNE23_RS21445 (SNE23_21445) aroK 4132580..4133077 (-) 498 WP_048531135.1 shikimate kinase AroK -
  SNE23_RS21450 (SNE23_21450) - 4133196..4133846 (-) 651 WP_048531136.1 2OG-Fe(II) oxygenase -
  SNE23_RS21455 (SNE23_21455) comGG 4134024..4134395 (-) 372 WP_071757287.1 competence type IV pilus minor pilin ComGG -
  SNE23_RS21460 (SNE23_21460) comGF 4134392..4134862 (-) 471 WP_157409059.1 competence type IV pilus minor pilin ComGF -
  SNE23_RS21465 (SNE23_21465) comGE 4134832..4135134 (-) 303 WP_071757286.1 competence type IV pilus minor pilin ComGE -
  SNE23_RS21470 (SNE23_21470) comGD 4135127..4135582 (-) 456 WP_048531144.1 comG operon protein ComGD -
  SNE23_RS21475 (SNE23_21475) comGC 4135579..4135878 (-) 300 WP_157409060.1 competence type IV pilus major pilin ComGC -
  SNE23_RS21480 (SNE23_21480) comGB 4135890..4136921 (-) 1032 WP_053082876.1 competence type IV pilus assembly protein ComGB -
  SNE23_RS21485 (SNE23_21485) comGA 4136914..4137957 (-) 1044 WP_048531150.1 competence protein ComGA Machinery gene
  SNE23_RS21490 (SNE23_21490) - 4138163..4138858 (+) 696 WP_071757285.1 helix-turn-helix transcriptional regulator -
  SNE23_RS21495 (SNE23_21495) - 4138984..4139226 (+) 243 WP_000440719.1 DUF2626 domain-containing protein -
  SNE23_RS21500 (SNE23_21500) - 4139334..4140728 (+) 1395 WP_001094339.1 L-cystine transporter -
  SNE23_RS21505 (SNE23_21505) - 4140945..4141160 (-) 216 WP_071757284.1 DUF3912 family protein -
  SNE23_RS21510 (SNE23_21510) - 4141414..4141725 (+) 312 WP_001093241.1 hypothetical protein -
  SNE23_RS21515 (SNE23_21515) - 4141766..4142254 (-) 489 WP_071757283.1 hypothetical protein -
  SNE23_RS21520 (SNE23_21520) - 4142334..4142708 (-) 375 WP_071757282.1 nucleoside 2-deoxyribosyltransferase -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39378.84 Da        Isoelectric Point: 8.8395

>NTDB_id=910005 SNE23_RS21485 WP_048531150.1 4136914..4137957(-) (comGA) [Bacillus sp. RA(2023)]
MNGIESFANMILKEACRVQASDLHIVPRKKDVAVQLRIGKDLMTKQYIEKEFGEKLVSHFKFLASMDIGERRKPQNGSLY
LQMDGQEVYLRLSTLPTVYQESLVIRLHLQASIQPLSHLSLFPSTAKKLLSFLHYSHGLLVFTGPTGSGKTTTMYALLEV
IRKKKTRRIVTLEDPVEKRNDDVLQIQINEKAGITYEAGLKAILRHDPDIILVGEIRDEETAKIAIRASLTGHLVMTTLH
TNDAKGAILRFMDFGITRQEIEQSLLAIAAQRLVELKCPFCKRKCSTLCKSMRQVRQASIYELLYGYELKQAIKEANGEC
VTYEHETLESSLRKGYALGFLEEDVYV

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=910005 SNE23_RS21485 WP_048531150.1 4136914..4137957(-) (comGA) [Bacillus sp. RA(2023)]
ATGAATGGAATTGAAAGCTTTGCGAATATGATTTTGAAAGAGGCGTGTAGGGTACAAGCTTCGGATTTACACATCGTGCC
CCGGAAGAAGGATGTTGCGGTTCAACTACGTATAGGAAAAGATTTAATGACGAAACAATACATTGAAAAGGAATTTGGAG
AAAAACTTGTTTCACACTTTAAATTTTTAGCATCCATGGATATAGGGGAGAGGCGGAAGCCACAAAATGGTTCATTGTAT
TTACAAATGGATGGACAAGAAGTGTATTTGCGCCTTTCCACGCTTCCAACCGTATATCAAGAAAGTCTCGTTATTCGTCT
TCATTTACAAGCATCTATTCAGCCGTTATCTCATCTTTCGTTATTTCCAAGTACAGCGAAGAAACTTCTTTCCTTTTTAC
ATTATTCGCATGGGTTACTCGTCTTTACGGGACCGACTGGTTCAGGAAAGACAACAACAATGTATGCATTATTAGAGGTA
ATTAGAAAAAAGAAAACACGTCGCATCGTTACACTGGAAGATCCAGTTGAAAAAAGAAATGACGATGTATTACAAATTCA
AATAAATGAAAAAGCAGGTATCACATATGAGGCGGGACTGAAGGCTATTTTACGCCATGATCCAGATATTATTTTAGTCG
GTGAAATTCGTGATGAAGAAACAGCAAAAATTGCTATAAGAGCAAGTTTGACTGGACATTTAGTAATGACGACATTGCAT
ACGAATGATGCGAAAGGAGCGATACTTCGGTTTATGGACTTTGGCATAACAAGGCAAGAGATTGAACAATCTTTATTGGC
TATAGCTGCACAGCGACTTGTCGAATTGAAGTGTCCGTTTTGCAAAAGAAAGTGCTCAACTTTATGTAAATCAATGAGGC
AGGTAAGACAAGCGAGTATTTATGAGTTGTTATATGGATATGAGTTAAAACAAGCGATTAAAGAAGCAAACGGGGAATGT
GTCACATACGAGCATGAGACATTAGAATCTTCGCTACGAAAAGGATACGCTTTAGGATTTTTAGAAGAGGATGTGTATGT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

57.925

100

0.579
  pilB Vibrio campbellii strain DS40M4

35.977

100

0.366