Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   SNE23_RS20290 Genome accession   NZ_CP139483
Coordinates   3934831..3935265 (-) Length   144 a.a.
NCBI ID   WP_000811502.1    Uniprot ID   A0A5C5ACH0
Organism   Bacillus sp. RA(2023)     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3929831..3940265
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SNE23_RS20270 (SNE23_20270) - 3930272..3930829 (+) 558 WP_071757371.1 PadR family transcriptional regulator -
  SNE23_RS20275 (SNE23_20275) - 3930978..3931409 (-) 432 WP_157409029.1 NUDIX hydrolase -
  SNE23_RS20280 (SNE23_20280) metE 3932080..3934368 (+) 2289 WP_071757369.1 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -
  SNE23_RS20285 (SNE23_20285) comJ 3934406..3934795 (-) 390 WP_048530770.1 competence protein ComJ -
  SNE23_RS20290 (SNE23_20290) nucA/comI 3934831..3935265 (-) 435 WP_000811502.1 DNA-entry nuclease Machinery gene
  SNE23_RS20295 (SNE23_20295) - 3935502..3936251 (+) 750 WP_157409030.1 DUF3967 domain-containing protein -
  SNE23_RS20300 (SNE23_20300) kinB 3936305..3937579 (-) 1275 WP_048530774.1 sporulation sensor histidine kinase KinB -
  SNE23_RS20305 (SNE23_20305) - 3937888..3938115 (+) 228 WP_321529480.1 hypothetical protein -
  SNE23_RS20310 (SNE23_20310) - 3938239..3939402 (+) 1164 WP_048530776.1 aminotransferase A -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16010.38 Da        Isoelectric Point: 8.4331

>NTDB_id=910002 SNE23_RS20290 WP_000811502.1 3934831..3935265(-) (nucA/comI) [Bacillus sp. RA(2023)]
MKQLKGIIISIIAILSIVVAVYEVLVPEETSIKKTNAYDQVLEFPKERYPETGKHITDAIKEGHSEVCTIDRGGAADRRK
LSLAPYPSKKGYDRDEWPMAMCKEGGKGAHIEYISPADNRGAGSWVGNKLDKYPDGTRVKFDVK

Nucleotide


Download         Length: 435 bp        

>NTDB_id=910002 SNE23_RS20290 WP_000811502.1 3934831..3935265(-) (nucA/comI) [Bacillus sp. RA(2023)]
ATGAAGCAATTAAAAGGTATTATCATTTCAATTATTGCAATTCTTTCTATTGTAGTAGCGGTGTATGAAGTACTTGTTCC
AGAGGAAACAAGTATCAAAAAAACAAATGCGTATGATCAAGTTCTAGAATTTCCGAAAGAGCGATATCCAGAGACAGGAA
AACATATTACGGATGCGATAAAAGAAGGGCATTCAGAAGTTTGTACAATCGACCGTGGTGGCGCTGCGGATAGAAGAAAA
TTATCGTTAGCTCCATATCCATCAAAAAAAGGATATGATCGTGATGAATGGCCCATGGCGATGTGTAAAGAAGGCGGAAA
AGGAGCACATATTGAATATATAAGTCCAGCAGATAACCGCGGAGCAGGCTCTTGGGTAGGAAATAAGTTAGATAAATACC
CAGATGGTACGCGTGTGAAGTTTGACGTAAAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(48-139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5C5ACH0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

71.171

77.083

0.549