Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   SNE23_RS00530 Genome accession   NZ_CP139483
Coordinates   97536..98912 (+) Length   458 a.a.
NCBI ID   WP_157409255.1    Uniprot ID   -
Organism   Bacillus sp. RA(2023)     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 92536..103912
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SNE23_RS00510 (SNE23_00510) ctsR 92729..93190 (+) 462 WP_048526354.1 transcriptional regulator CtsR -
  SNE23_RS00515 (SNE23_00515) - 93364..93912 (+) 549 WP_048526355.1 UvrB/UvrC motif-containing protein -
  SNE23_RS00520 (SNE23_00520) - 93917..94981 (+) 1065 WP_048526356.1 protein arginine kinase -
  SNE23_RS00525 (SNE23_00525) clpC 95004..97439 (+) 2436 WP_000971179.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  SNE23_RS00530 (SNE23_00530) radA 97536..98912 (+) 1377 WP_157409255.1 DNA repair protein RadA Machinery gene
  SNE23_RS00535 (SNE23_00535) disA 98916..99989 (+) 1074 WP_048526357.1 DNA integrity scanning diadenylate cyclase DisA -
  SNE23_RS00540 (SNE23_00540) - 100150..101259 (+) 1110 WP_048526358.1 PIN/TRAM domain-containing protein -
  SNE23_RS00545 (SNE23_00545) ispD 101276..101956 (+) 681 WP_071757382.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  SNE23_RS00550 (SNE23_00550) ispF 102072..102548 (+) 477 WP_048526360.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 49812.42 Da        Isoelectric Point: 7.1421

>NTDB_id=909977 SNE23_RS00530 WP_157409255.1 97536..98912(+) (radA) [Bacillus sp. RA(2023)]
MAKKKTKFTCQECGYQSPKYMGKCPGCGQWNTLVEEMEPVVSSRRLNYANAIQSEVTKPRRLTEVETKSEARIETKFQEF
NRVLGGGIVDGSLVLIGGDPGIGKSTLLLQISSQLADSSYDVLYISGEESAKQIKLRADRLHVNGSNLFVVSETDLQRIA
AHIEEVNPVFVVIDSIQTIHLPEVTSAPGSVAQVRECTAELMKLAKTKGIPIFIVGHVTKEGAIAGPRMLEHMVDAVLYF
EGDRHHTYRILRAVKNRFGSTNEMGIFEMKELGLAEVLNPSEIFLEERPVGVAGSTVVASMEGTRPVLVEIQALISPTSF
GNPRRMATGIDHNRVSLIMAVLEKRTGLLLQNQDAYLKVAGGLKLDEPAIDLAVALSIASSFRDKSTAPADAVIGEVGLT
GEIRRVSRIEQRVQEAAKLGFQRAIIPRKNLGGWTIPDGIEVVGVSNLGEALRLTLGG

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=909977 SNE23_RS00530 WP_157409255.1 97536..98912(+) (radA) [Bacillus sp. RA(2023)]
ATGGCTAAAAAGAAAACAAAATTCACATGTCAAGAGTGTGGGTATCAGTCACCAAAATATATGGGGAAATGCCCTGGGTG
TGGTCAATGGAATACGCTTGTTGAAGAGATGGAACCAGTTGTATCATCAAGGCGCCTTAATTATGCCAATGCGATTCAAT
CAGAAGTAACAAAACCAAGACGTCTAACAGAAGTAGAAACAAAGTCTGAGGCGCGTATTGAAACGAAATTTCAAGAATTT
AACCGCGTACTTGGTGGCGGGATTGTAGATGGGTCCTTAGTACTTATTGGTGGTGACCCTGGGATTGGAAAATCAACGTT
ACTATTACAAATATCATCGCAATTAGCAGATTCTTCATATGATGTACTATATATATCGGGTGAAGAATCAGCAAAACAGA
TTAAACTGCGTGCGGACCGTTTACATGTAAATGGTAGCAATCTATTTGTTGTATCAGAGACGGATTTGCAGCGGATTGCA
GCACACATTGAAGAAGTGAATCCAGTCTTTGTTGTTATTGATTCTATCCAAACGATACATTTACCTGAGGTAACGTCAGC
ACCAGGAAGTGTGGCGCAAGTACGTGAATGTACAGCGGAATTAATGAAACTTGCAAAAACGAAAGGGATCCCTATTTTTA
TCGTTGGACATGTGACAAAAGAGGGGGCAATTGCAGGGCCTCGTATGTTAGAACATATGGTCGATGCAGTTCTTTATTTT
GAAGGAGACCGCCATCATACATATCGTATTTTGCGAGCGGTGAAGAATCGTTTTGGTTCCACGAATGAAATGGGTATTTT
TGAAATGAAAGAACTTGGCCTTGCGGAAGTATTAAATCCTTCTGAAATTTTCCTTGAGGAAAGACCAGTTGGGGTTGCAG
GGTCAACGGTGGTTGCCTCAATGGAAGGAACACGACCAGTTTTAGTAGAAATACAAGCATTAATCTCCCCTACTAGTTTT
GGAAACCCTCGAAGAATGGCAACGGGAATTGACCATAACCGTGTATCGCTTATTATGGCAGTGTTAGAAAAAAGAACAGG
TTTATTATTGCAAAACCAAGACGCATATTTAAAAGTAGCAGGTGGTCTGAAGTTAGATGAACCAGCAATTGATTTAGCTG
TGGCTTTAAGTATAGCTTCAAGTTTTAGAGATAAATCTACGGCACCAGCCGATGCAGTAATAGGAGAAGTAGGATTAACT
GGAGAAATAAGAAGAGTGTCAAGAATTGAACAACGTGTACAAGAAGCAGCTAAATTAGGATTTCAACGCGCTATTATTCC
TAGAAAAAATTTAGGGGGATGGACAATTCCTGATGGGATTGAAGTAGTAGGTGTATCCAATTTAGGAGAAGCGCTTCGTT
TGACATTAGGAGGCTAG

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(60-222)

Subunit ChlI of Mg-chelatase (ChlI)

(363-430)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

74.946

100

0.751
  radA Streptococcus pneumoniae Rx1

63.797

98.908

0.631
  radA Streptococcus pneumoniae D39

63.797

98.908

0.631
  radA Streptococcus pneumoniae R6

63.797

98.908

0.631
  radA Streptococcus pneumoniae TIGR4

63.797

98.908

0.631
  radA Streptococcus mitis SK321

63.797

98.908

0.631
  radA Streptococcus mitis NCTC 12261

63.576

98.908

0.629