NTDB

Overview

Name	pilT	Type	Machinery gene
Locus tag	SR933_RS15280	Genome accession	NZ_CP139472
Coordinates	3269214..3270248 (-)	Length	344 a.a.
NCBI ID	WP_013333867.1	Uniprot ID	E1VAP8
Organism	Halomonas elongata DSM 2581 strain ATCC 33173
Function	type IV pilus retraction (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3264214..3275248

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SR933_RS15270 (SR933_15270)	tyrS	3266764..3267963 (-)	1200	WP_013333865.1	tyrosine--tRNA ligase	-
SR933_RS15275 (SR933_15275)	-	3268075..3269205 (+)	1131	WP_013333866.1	anhydro-N-acetylmuramic acid kinase	-
SR933_RS15280 (SR933_15280)	pilT	3269214..3270248 (-)	1035	WP_013333867.1	type IV pilus twitching motility protein PilT	Machinery gene
SR933_RS15285 (SR933_15285)	-	3270305..3271009 (+)	705	WP_013333868.1	YggS family pyridoxal phosphate-dependent enzyme	-
SR933_RS15290 (SR933_15290)	proC	3271070..3271894 (+)	825	WP_013333869.1	pyrroline-5-carboxylate reductase	-
SR933_RS15295 (SR933_15295)	-	3271921..3272514 (+)	594	WP_013333870.1	YggT family protein	-
SR933_RS15300 (SR933_15300)	-	3272557..3273714 (+)	1158	WP_013333871.1	homoserine O-acetyltransferase	-
SR933_RS15305 (SR933_15305)	metW	3273714..3274340 (+)	627	WP_041602709.1	methionine biosynthesis protein MetW	-
SR933_RS15310 (SR933_15310)	sfsA	3274347..3275078 (-)	732	WP_013333873.1	DNA/RNA nuclease SfsA	-

Sequence

Protein

Download Length: 344 a.a. Molecular weight: 36829.38 Da Isoelectric Point: 6.6657

                                >NTDB_id=909924 SR933_RS15280 WP_013333867.1 3269214..3270248(-) (pilT) [Halomonas elongata DSM 2581 strain ATCC 33173]

MNITELLAASAKRGASDLHLSAGMAPMMRVDGELEALDGPPVDDPTLRRELRRLLGESHWATFESELEVDVALELPGVAR

FRGNVFQHGRGIGAVFRTIPGDVPGLAALGMADTLGALAELRRGLVLVTGPTGSGKSTTLAALVNAINETRREHILTIED

PVEFVHHGKRCLVSQREVSRDTRSFAAALRSALREDPDVIMVGEMRDLETMRLALTAAETGHLVLGTLHTLSAAKTIDRI

IDAFPGPEQGMVRAMLSESLQAVVSQVLLKRCGGGRVAAHEVMVATPAVRNLIREDKVAQLHSVLQTGAAQGMQTLDTAL

ARLVAEGVVAPDEAQSRARAPLRR

Nucleotide

Download Length: 1035 bp

                                >NTDB_id=909924 SR933_RS15280 WP_013333867.1 3269214..3270248(-) (pilT) [Halomonas elongata DSM 2581 strain ATCC 33173]

ATGAATATTACCGAACTGCTGGCAGCCTCGGCAAAGCGGGGCGCCTCCGATCTGCATCTGTCGGCAGGCATGGCACCGAT

GATGCGCGTCGACGGCGAGCTGGAAGCGCTCGATGGGCCGCCCGTCGATGACCCGACCTTGCGCAGAGAACTTCGAAGAC

TGCTCGGTGAGTCGCATTGGGCAACCTTCGAGTCCGAGTTGGAAGTCGACGTTGCCCTGGAGCTTCCCGGCGTGGCGCGT

TTCCGGGGCAACGTCTTCCAGCATGGCCGGGGCATCGGCGCGGTGTTTCGCACCATCCCCGGCGATGTGCCTGGACTGGC

TGCGCTCGGCATGGCGGATACCCTGGGGGCTCTGGCCGAACTGCGACGCGGACTGGTGCTGGTGACGGGGCCCACTGGTT

CGGGCAAGAGCACGACCCTGGCGGCGCTGGTCAATGCCATCAACGAGACGCGCCGCGAGCACATCCTGACCATCGAGGAT

CCGGTGGAGTTCGTGCATCACGGCAAGCGGTGCCTGGTCAGCCAGCGCGAGGTGTCGCGGGATACCCGGAGCTTTGCGGC

GGCCTTGCGCAGCGCGCTGCGCGAGGATCCCGATGTCATCATGGTCGGTGAAATGCGCGACCTGGAGACCATGCGGCTGG

CGCTGACTGCCGCCGAGACGGGGCACCTGGTACTGGGAACCCTGCATACGCTCTCGGCGGCCAAGACCATCGATCGCATC

ATCGATGCCTTCCCCGGCCCCGAACAGGGCATGGTGCGGGCGATGCTGTCGGAATCCCTGCAGGCGGTCGTCTCGCAGGT

GCTGTTGAAGCGCTGTGGGGGCGGGCGCGTGGCGGCTCATGAAGTCATGGTCGCCACGCCGGCGGTGCGTAACCTGATTC

GCGAGGACAAGGTGGCGCAACTCCATTCGGTGCTGCAGACCGGCGCCGCCCAGGGCATGCAGACACTGGACACGGCGCTG

GCGCGGCTGGTGGCCGAGGGTGTGGTGGCGCCTGACGAGGCGCAGTCGCGGGCGCGCGCTCCCCTGAGGCGGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(13-268)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
AlphaFold DB	E1VAP8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
pilT	Acinetobacter baumannii D1279779	62.17	99.128	0.616
pilT	Acinetobacter baumannii strain A118	62.17	99.128	0.616
pilT	Pseudomonas stutzeri DSM 10701	62.17	99.128	0.616
pilT	Pseudomonas aeruginosa PAK	61.877	99.128	0.613
pilT	Acinetobacter nosocomialis M2	61.877	99.128	0.613
pilT	Acinetobacter baylyi ADP1	60.704	99.128	0.602
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	60.35	99.709	0.602
pilT	Vibrio cholerae strain A1552	60.35	99.709	0.602
pilT	Neisseria meningitidis 8013	59.118	98.837	0.584
pilT	Neisseria gonorrhoeae MS11	58.824	98.837	0.581
pilT	Legionella pneumophila strain Lp02	58.284	98.256	0.573
pilT	Legionella pneumophila strain ERS1305867	58.284	98.256	0.573
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	48.538	99.419	0.483
pilU	Pseudomonas stutzeri DSM 10701	39.701	97.384	0.387
pilU	Vibrio cholerae strain A1552	38.806	97.384	0.378
pilB	Acinetobacter baylyi ADP1	32.061	100	0.366
pilU	Acinetobacter baylyi ADP1	36.152	99.709	0.36

Detailed information NTG0909924