Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   NCY62_RS15660 Genome accession   NZ_CP139356
Coordinates   3261332..3262132 (-) Length   266 a.a.
NCBI ID   WP_212808517.1    Uniprot ID   -
Organism   Acinetobacter pittii strain A45P     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3256332..3267132
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NCY62_RS15640 (NCY62_15695) - 3257397..3258095 (-) 699 WP_057077318.1 YfgM family protein -
  NCY62_RS15645 (NCY62_15700) hisS 3258124..3259416 (-) 1293 WP_057077317.1 histidine--tRNA ligase -
  NCY62_RS15650 (NCY62_15705) ispG 3259413..3260528 (-) 1116 WP_002115078.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  NCY62_RS15655 (NCY62_15710) - 3260550..3261341 (-) 792 WP_002114935.1 helix-turn-helix domain-containing protein -
  NCY62_RS15660 (NCY62_15715) pilF 3261332..3262132 (-) 801 WP_212808517.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  NCY62_RS15665 (NCY62_15720) rlmN 3262147..3263382 (-) 1236 WP_002115097.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  NCY62_RS15670 (NCY62_15725) ndk 3263506..3263937 (-) 432 WP_250733677.1 nucleoside-diphosphate kinase -
  NCY62_RS15675 (NCY62_15730) iscX 3264051..3264251 (-) 201 WP_004698764.1 Fe-S cluster assembly protein IscX -
  NCY62_RS15680 (NCY62_15735) - 3264351..3264902 (-) 552 WP_057077315.1 phosphatase PAP2 family protein -
  NCY62_RS15685 (NCY62_15740) - 3264927..3266216 (-) 1290 WP_081038253.1 glycosyltransferase family 4 protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30141.40 Da        Isoelectric Point: 9.0417

>NTDB_id=909084 NCY62_RS15660 WP_212808517.1 3261332..3262132(-) (pilF) [Acinetobacter pittii strain A45P]
MSIPKLKTVLCMGIAVAFLVSGCQTTHTQKKDPEKAVKVRTQLAAEHIRSGDLDSAKRALDQALNVDSRDATANMMMGIL
LQQEGSKPNLEKAEHYFKRAISSEPDNAQAHNNYGTYLYQMERYNDAVEQFRIAGATLGYDQRYQALENLGRIYLKLGDV
ANAEKTFKQALLANRDSYISMLELAEIFYLQQQIPAATQMYEQYVRTVGQKNQGARALWIGVRVARANADKMGMQVLVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=909084 NCY62_RS15660 WP_212808517.1 3261332..3262132(-) (pilF) [Acinetobacter pittii strain A45P]
TTGAGTATTCCAAAGTTAAAAACGGTCTTATGTATGGGGATTGCAGTTGCATTTCTGGTAAGTGGTTGCCAGACAACGCA
TACGCAAAAAAAAGATCCAGAGAAAGCGGTAAAGGTGCGTACACAACTTGCTGCTGAACATATCCGTTCGGGTGACTTGG
ACTCAGCAAAGCGAGCTCTTGATCAAGCTTTAAATGTAGATAGCCGCGATGCTACTGCAAATATGATGATGGGTATTTTG
CTACAGCAAGAAGGTAGCAAACCCAATTTAGAAAAAGCAGAACATTATTTTAAACGAGCAATTTCATCTGAACCGGATAA
TGCTCAAGCACATAATAATTATGGTACTTATTTATATCAAATGGAGCGTTATAATGATGCGGTTGAGCAATTTCGTATTG
CTGGCGCAACATTAGGTTATGACCAACGCTATCAGGCATTAGAAAATCTGGGGCGTATTTATTTAAAATTGGGTGATGTT
GCGAATGCTGAAAAAACATTCAAACAAGCACTCCTTGCTAACCGCGACTCTTATATTTCAATGTTAGAGTTAGCGGAAAT
TTTTTATTTGCAGCAGCAGATTCCGGCTGCTACACAAATGTATGAGCAATATGTTCGTACAGTGGGGCAGAAAAATCAGG
GTGCAAGAGCACTTTGGATTGGTGTAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAATGCAAGTGTTAGTAAACCAG
TTACGTGCTTTGTTCCCAGAAAGTCCAGAATATCAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_16)

(89-134)

Tetratricopeptide repeat (TPR_16)

(149-206)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

93.609

100

0.936
  pilF Acinetobacter baylyi ADP1

76.154

97.744

0.744