Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGC/cglC   Type   Machinery gene
Locus tag   EFK4_RS08450 Genome accession   NZ_AP025270
Coordinates   1735255..1735530 (-) Length   91 a.a.
NCBI ID   WP_002356991.1    Uniprot ID   A0A1B4XPV0
Organism   Enterococcus faecalis strain K-4     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1730255..1740530
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EFK4_RS08415 (EFK4_16440) - 1730810..1731283 (+) 474 WP_002357065.1 universal stress protein -
  EFK4_RS08420 (EFK4_16450) - 1731395..1732582 (-) 1188 WP_002357064.1 acetate/propionate family kinase -
  EFK4_RS08425 (EFK4_16460) - 1732607..1733614 (-) 1008 WP_002357063.1 class I SAM-dependent methyltransferase -
  EFK4_RS08430 (EFK4_16470) comGG 1733743..1734096 (-) 354 WP_002357061.1 competence type IV pilus minor pilin ComGG -
  EFK4_RS08435 (EFK4_16480) comGF 1734096..1734530 (-) 435 WP_002357060.1 competence type IV pilus minor pilin ComGF -
  EFK4_RS08440 (EFK4_16490) - 1734520..1734846 (-) 327 WP_002393943.1 type II secretion system protein -
  EFK4_RS08445 (EFK4_16500) comGD 1734812..1735258 (-) 447 WP_002379576.1 competence type IV pilus minor pilin ComGD -
  EFK4_RS08450 (EFK4_16510) comGC/cglC 1735255..1735530 (-) 276 WP_002356991.1 competence type IV pilus major pilin ComGC Machinery gene
  EFK4_RS08455 (EFK4_16520) comGB 1735530..1736576 (-) 1047 WP_002356990.1 competence type IV pilus assembly protein ComGB -
  EFK4_RS08460 (EFK4_16530) comGA 1736533..1737501 (-) 969 WP_002364362.1 competence type IV pilus ATPase ComGA -
  EFK4_RS08465 (EFK4_16540) - 1737742..1739070 (-) 1329 WP_002356988.1 APC family permease -
  EFK4_RS08470 (EFK4_16550) rlmN 1739360..1740433 (-) 1074 WP_010708818.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -

Sequence

Protein


Download         Length: 91 a.a.        Molecular weight: 10464.41 Da        Isoelectric Point: 9.3192

>NTDB_id=90884 EFK4_RS08450 WP_002356991.1 1735255..1735530(-) (comGC/cglC) [Enterococcus faecalis strain K-4]
MKKKQKYAGFTLLEMLIVLLIISVLILLFVPNLAKHKETVDKKGNEAIVKIVESQIELYTLEKNKTPSLNELVNEGYITK
EQLDKYTAEKQ

Nucleotide


Download         Length: 276 bp        

>NTDB_id=90884 EFK4_RS08450 WP_002356991.1 1735255..1735530(-) (comGC/cglC) [Enterococcus faecalis strain K-4]
ATGAAAAAGAAACAAAAATACGCAGGGTTTACATTATTAGAAATGTTGATTGTCTTATTGATTATTTCCGTATTGATTTT
ACTTTTTGTCCCTAACTTAGCGAAACATAAAGAAACAGTTGATAAAAAAGGCAATGAAGCAATCGTAAAAATTGTAGAAT
CACAAATCGAGCTCTACACACTAGAAAAAAATAAGACGCCTTCCTTAAATGAATTAGTCAACGAAGGCTACATTACTAAA
GAGCAGTTAGATAAATATACAGCAGAAAAGCAATGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(5-28)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1B4XPV0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGC/cglC Streptococcus pneumoniae Rx1

63.095

92.308

0.582
  comGC/cglC Streptococcus pneumoniae D39

63.095

92.308

0.582
  comGC/cglC Streptococcus pneumoniae R6

63.095

92.308

0.582
  comGC/cglC Streptococcus pneumoniae TIGR4

63.095

92.308

0.582
  comGC/cglC Streptococcus mitis NCTC 12261

61.905

92.308

0.571
  comGC/cglC Streptococcus mitis SK321

61.176

93.407

0.571
  comGC Lactococcus lactis subsp. cremoris KW2

58.14

94.505

0.549
  comYC Streptococcus gordonii str. Challis substr. CH1

56.322

95.604

0.538
  comYC Streptococcus suis isolate S10

52.326

94.505

0.495
  comYC Streptococcus mutans UA159

57.692

85.714

0.495
  comYC Streptococcus mutans UA140

57.692

85.714

0.495
  comGC Latilactobacillus sakei subsp. sakei 23K

46.154

100

0.462
  comGC Staphylococcus aureus MW2

46.835

86.813

0.407
  comGC Staphylococcus aureus N315

46.835

86.813

0.407
  comGC Bacillus subtilis subsp. subtilis str. 168

48.649

81.319

0.396

Multiple sequence alignment