Detailed information NTG0908697

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: SOI75_RS09205
• Genome accession: NZ_CP139282
• Coordinates: 1888635..1889753 (+)
• Length: 372 a.a.
• NCBI ID: WP_005803129.1
• Uniprot ID: A0A009MM04
• Organism: Acinetobacter pittii strain AE13
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1883635..1894753

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SOI75_RS09195 (SOI75_09195)	yggS	1886759..1887451 (-)	693	WP_005803127.1	YggS family pyridoxal phosphate-dependent enzyme	-
SOI75_RS09200 (SOI75_09200)	pilT	1887578..1888615 (+)	1038	WP_002121749.1	type IV pilus twitching motility protein PilT	Machinery gene
SOI75_RS09205 (SOI75_09205)	pilU	1888635..1889753 (+)	1119	WP_005803129.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
SOI75_RS09210 (SOI75_09210)	fur	1889823..1890260 (-)	438	WP_002121758.1	ferric iron uptake transcriptional regulator	-
SOI75_RS09215 (SOI75_09215)	omlA	1890372..1890770 (+)	399	WP_002121740.1	outer membrane protein assembly factor BamE	-
SOI75_RS09220 (SOI75_09220)	-	1890835..1891149 (-)	315	WP_017481335.1	RnfH family protein	-
SOI75_RS09225 (SOI75_09225)	-	1891149..1892216 (-)	1068	WP_031944146.1	membrane protein	-
SOI75_RS09230 (SOI75_09230)	-	1892370..1892825 (-)	456	WP_002121698.1	bacteriohemerythrin	-
SOI75_RS09235 (SOI75_09235)	-	1893077..1893925 (-)	849	WP_005803134.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41903.09 Da
• Isoelectric Point: 6.6403

>NTDB_id=908697 SOI75_RS09205 WP_005803129.1 1888635..1889753(+) (pilU) [Acinetobacter pittii strain AE13]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEIIGQLLHSIMSEKQRKEFAETRECNFAIMNRDKSA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISYRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKSGQITYKDALKHADSPNDLRLTIKLAEEGPDQIAGTNQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=908697 SOI75_RS09205 WP_005803129.1 1888635..1889753(+) (pilU) [Acinetobacter pittii strain AE13]
ATGGATTTTAATGACCTGCTCAACCTGATGGTTGAAAAAAAATCATCCGACCTCTTTATTACAGATGGCGTTGCACCATC
GATGAAGATTAACGGGCAAATTGTTCCCATTTCAAAAAATAGTCTTTCAGGCGAAATTATCGGGCAACTATTACACTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGAGACTCGTGAATGTAACTTTGCGATTATGAACCGCGATAAAAGTGCG
CGCTTTCGTGTAAGTGCTTTTCAGCAACGCGACATGCCAGGCATGGTACTACGTCGAATTGAAACCAAAATTCCTTCAAT
TGACGACTTACAATTACCGCCAGTGCTTAAAGATTTATCGATGACCAAACGTGGCATCATTATTTTTGTAGGGGCAACAG
GTACAGGTAAATCGACATCATTGGCTTCAATGATTAGCTATCGTAACCATAACTCTAAAGGTCATATCATTACCATTGAA
GACCCTATCGAGTTTATTCATGAACACGCTGGCTGCATCATTACCCAACGTGAAGTCGGGATCGATACAGACTCATTTGA
AATTGCTTTAAAGAATACGTTGCGACAAGCACCCGATGTAATCTTGATTGGTGAGATTCGCTCTCGTGAAGTTATGGACT
ACGCGATTGGCTTCGCCGAAACAGGTCACCTTGTATTAGCCACAATGCATGCGAACAATGCCAACCAAGCACTTGACCGT
ATTATTCACTTCTTTGAAAGTGACCGTCATAGCCAGCTCTACATGGATTTATCACTTAACTTAAAGGCCATGATTGCACA
GCAACTTATTCCGACTCCAGATGGTAATTCGCGCCGTGCAGCGATTGAGATTCTCATTAACTCACCATTAATTTCAGACT
ATATTCGTAAAGGCGAAATTCATGAAATTAAAGATTTAATGAAACGCTCACGTGAACTCGGTATGCAAACTTTTGACCAA
GCACTGTTTGATCTTTATAAGTCAGGCCAAATTACCTACAAAGATGCATTAAAACATGCCGACTCACCAAACGATTTGCG
TTTAACAATTAAGCTTGCCGAAGAAGGTCCAGATCAAATTGCAGGCACAAATCAGCATTTAACCTTCGACCGACAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (32-270)

3D structure

• AlphaFold DB: A0A009MM04

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.397	100	0.766
pilU	Pseudomonas stutzeri DSM 10701	68.464	99.731	0.683
pilU	Vibrio cholerae strain A1552	54.324	99.462	0.54
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	40.934	97.849	0.401
pilT	Legionella pneumophila strain Lp02	39.941	90.86	0.363
pilT	Legionella pneumophila strain ERS1305867	39.941	90.86	0.363