Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   SOI77_RS16720 Genome accession   NZ_CP139275
Coordinates   3521995..3522795 (-) Length   266 a.a.
NCBI ID   WP_213030436.1    Uniprot ID   -
Organism   Acinetobacter pittii strain HCG138     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3516995..3527795
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SOI77_RS16700 (SOI77_16700) - 3518060..3518758 (-) 699 WP_014208343.1 YfgM family protein -
  SOI77_RS16705 (SOI77_16705) hisS 3518787..3520079 (-) 1293 WP_017398815.1 histidine--tRNA ligase -
  SOI77_RS16710 (SOI77_16710) ispG 3520076..3521191 (-) 1116 WP_002115078.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  SOI77_RS16715 (SOI77_16715) - 3521213..3522004 (-) 792 WP_002114935.1 helix-turn-helix domain-containing protein -
  SOI77_RS16720 (SOI77_16720) pilF 3521995..3522795 (-) 801 WP_213030436.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  SOI77_RS16725 (SOI77_16725) rlmN 3522810..3524045 (-) 1236 WP_002115097.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  SOI77_RS16730 (SOI77_16730) ndk 3524169..3524600 (-) 432 WP_000963851.1 nucleoside-diphosphate kinase -
  SOI77_RS16735 (SOI77_16735) iscX 3524714..3524914 (-) 201 WP_004698764.1 Fe-S cluster assembly protein IscX -
  SOI77_RS16740 (SOI77_16740) - 3525014..3525565 (-) 552 WP_289518692.1 phosphatase PAP2 family protein -
  SOI77_RS16745 (SOI77_16745) - 3525590..3526879 (-) 1290 WP_270906812.1 glycosyltransferase family 4 protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30100.35 Da        Isoelectric Point: 9.0417

>NTDB_id=908626 SOI77_RS16720 WP_213030436.1 3521995..3522795(-) (pilF) [Acinetobacter pittii strain HCG138]
MSIPKLKTVLCMGIAVAFLASGCQTTHTQKKDPEKAVKVRTQLAAEHIRSGDLDSAKRALDQALSVDSRDATANMMMGIL
LQQEGSKPNLEKAEHYFKRAISSEPDNAQAHNNYGTYLYQMERYNEAVEQFRIAGATLGYDQRYQALENLGRIYLKLGDV
ANAEKTFKQALLANRDSYISMLELAEIFYLQQQIPAATQMYEQYVRTVGQKNQGARALWIGVRVARANADKMGMQVLVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=908626 SOI77_RS16720 WP_213030436.1 3521995..3522795(-) (pilF) [Acinetobacter pittii strain HCG138]
TTGAGTATTCCAAAGTTAAAAACGGTCTTATGTATGGGGATTGCAGTTGCATTTCTGGCAAGTGGTTGCCAGACAACGCA
TACGCAAAAAAAAGACCCAGAGAAAGCGGTAAAGGTGCGTACACAACTTGCTGCTGAACATATCCGTTCGGGTGACTTGG
ACTCAGCAAAGCGAGCTCTTGATCAAGCTTTAAGTGTAGATAGCCGCGATGCTACTGCAAATATGATGATGGGTATTTTG
CTACAGCAAGAAGGTAGCAAACCCAATTTAGAAAAAGCAGAACATTATTTCAAACGAGCAATTTCATCTGAACCGGATAA
TGCTCAAGCACATAATAATTATGGTACTTATTTATATCAAATGGAGCGTTATAATGAGGCTGTTGAGCAATTTCGTATTG
CTGGCGCAACATTAGGTTATGACCAACGCTATCAGGCATTAGAAAATCTGGGGCGTATTTATTTAAAATTGGGTGATGTT
GCGAATGCTGAAAAAACATTCAAACAAGCACTCCTTGCTAACCGCGACTCTTATATTTCAATGTTAGAGTTAGCGGAAAT
TTTTTATTTGCAGCAGCAGATTCCGGCTGCTACACAAATGTATGAGCAATATGTTCGTACAGTGGGGCAGAAAAATCAGG
GTGCAAGAGCACTTTGGATTGGTGTAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAATGCAAGTGTTAGTAAACCAG
TTACGTGCCTTGTTCCCGGAAAGTCCAGAATATCAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_16)

(149-206)

Tetratricopeptide repeat (TPR_16)

(89-134)

Tetratricopeptide repeat (TPR_8)

(39-68)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

93.985

100

0.94
  pilF Acinetobacter baylyi ADP1

75.769

97.744

0.741